Immobilization of 3-hydroxybenzoate 6-hydroxylase onto functionalized electrospun polycaprolactone ultrafine fibers: A novel heterogeneous catalyst

• Main Authors: Tiyaporn Srisook, Thammasit Vongsetskul, Jeerus Sucharitakul, Pimchai Chaiyen, Pramuan Tangboriboonrat
• Other Authors: Mahidol University
• Format: Article
• Published: 2018
• Subjects: Biochemistry, Genetics and Molecular Biology; Chemical Engineering; Chemistry; Environmental Science; Materials Science
• Online Access: https://repository.li.mahidol.ac.th/handle/123456789/33395

3-Hydroxybenzoate 6-hydroxylase (3HB6H), an enzyme that catalyzes regiospecific para-hydroxylation of aromatic compounds, was successfully immobilized onto electrospun polycaprolactone (PCL) ultrafine fibers (424 ± 99 nm in diameter). The fibers were fabricated from 13% w/w PCL (MW ∼8 × 104g/mol) di...