Viscoelastic property and cell adhesion process of cultured fibroblasts on different self-assembled monolayers monitored by acoustic wave biosensor

© Springer International Publishing Switzerland 2014. A quartz crystal microbalance (QCM) technique was used to reveal the effect of alkanethiol self-assembled monolayers (SAMs) on the viscoelastic property and cell adhesion process of cultured fibroblasts (L929), by measuring the change in frequenc...

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Main Authors: Y. Viturawong, S. Chongthammakun, N. Niamsiri, T. Srikhirin, T. Osotchan
其他作者: Mahidol University
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出版: 2018
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spelling th-mahidol.335792018-11-09T09:16:04Z Viscoelastic property and cell adhesion process of cultured fibroblasts on different self-assembled monolayers monitored by acoustic wave biosensor Y. Viturawong S. Chongthammakun N. Niamsiri T. Srikhirin T. Osotchan Mahidol University Chemical Engineering Engineering © Springer International Publishing Switzerland 2014. A quartz crystal microbalance (QCM) technique was used to reveal the effect of alkanethiol self-assembled monolayers (SAMs) on the viscoelastic property and cell adhesion process of cultured fibroblasts (L929), by measuring the change in frequency (∆f) and resistance (∆R). Four types of SAMs having different functional groups including hydroxyl (OH), carboxylic acid (COOH), amine (NH<inf>2</inf>) and methyl (CH<inf>3</inf>) were used in this study. The QCM measurements during cell adhesion showed that floating cells were rapidly adsorbed on all functionalized surfaces as a soft cell monolayer which their stiffness was increased by cell spreading process. Initially, the ∆f and ∆R were both positive shifts for COOH, OH and NH<inf>2</inf> surfaces indicated fluid-like behavior of adherent cells. A multi-step decreasing of both ∆f and ∆R were observed in case of COOH and OH surfaces represented solid-like behavior of cell spreading. The CH3 surface was used as a control because no cell adhesion occurs. QCM responses and morphological changes during cell adhesion process were resulted from viscoelastic nature of mammalian cells. The changes of cell morphology and viscoelastic property during cell adhesion process strongly depend on the surface functionality which can be observed by QCM technique. 2018-11-09T02:03:30Z 2018-11-09T02:03:30Z 2014-01-01 Conference Paper IFMBE Proceedings. Vol.43, (2014), 319-322 10.1007/978-3-319-02913-9_81 16800737 2-s2.0-84928235148 https://repository.li.mahidol.ac.th/handle/123456789/33579 Mahidol University SCOPUS https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84928235148&origin=inward
institution Mahidol University
building Mahidol University Library
continent Asia
country Thailand
Thailand
content_provider Mahidol University Library
collection Mahidol University Institutional Repository
topic Chemical Engineering
Engineering
spellingShingle Chemical Engineering
Engineering
Y. Viturawong
S. Chongthammakun
N. Niamsiri
T. Srikhirin
T. Osotchan
Viscoelastic property and cell adhesion process of cultured fibroblasts on different self-assembled monolayers monitored by acoustic wave biosensor
description © Springer International Publishing Switzerland 2014. A quartz crystal microbalance (QCM) technique was used to reveal the effect of alkanethiol self-assembled monolayers (SAMs) on the viscoelastic property and cell adhesion process of cultured fibroblasts (L929), by measuring the change in frequency (∆f) and resistance (∆R). Four types of SAMs having different functional groups including hydroxyl (OH), carboxylic acid (COOH), amine (NH<inf>2</inf>) and methyl (CH<inf>3</inf>) were used in this study. The QCM measurements during cell adhesion showed that floating cells were rapidly adsorbed on all functionalized surfaces as a soft cell monolayer which their stiffness was increased by cell spreading process. Initially, the ∆f and ∆R were both positive shifts for COOH, OH and NH<inf>2</inf> surfaces indicated fluid-like behavior of adherent cells. A multi-step decreasing of both ∆f and ∆R were observed in case of COOH and OH surfaces represented solid-like behavior of cell spreading. The CH3 surface was used as a control because no cell adhesion occurs. QCM responses and morphological changes during cell adhesion process were resulted from viscoelastic nature of mammalian cells. The changes of cell morphology and viscoelastic property during cell adhesion process strongly depend on the surface functionality which can be observed by QCM technique.
author2 Mahidol University
author_facet Mahidol University
Y. Viturawong
S. Chongthammakun
N. Niamsiri
T. Srikhirin
T. Osotchan
format Conference or Workshop Item
author Y. Viturawong
S. Chongthammakun
N. Niamsiri
T. Srikhirin
T. Osotchan
author_sort Y. Viturawong
title Viscoelastic property and cell adhesion process of cultured fibroblasts on different self-assembled monolayers monitored by acoustic wave biosensor
title_short Viscoelastic property and cell adhesion process of cultured fibroblasts on different self-assembled monolayers monitored by acoustic wave biosensor
title_full Viscoelastic property and cell adhesion process of cultured fibroblasts on different self-assembled monolayers monitored by acoustic wave biosensor
title_fullStr Viscoelastic property and cell adhesion process of cultured fibroblasts on different self-assembled monolayers monitored by acoustic wave biosensor
title_full_unstemmed Viscoelastic property and cell adhesion process of cultured fibroblasts on different self-assembled monolayers monitored by acoustic wave biosensor
title_sort viscoelastic property and cell adhesion process of cultured fibroblasts on different self-assembled monolayers monitored by acoustic wave biosensor
publishDate 2018
url https://repository.li.mahidol.ac.th/handle/123456789/33579
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