Label-free detection of meca-mediated methicillin-resistant staphylococcus aureus (MRSA) using surface plasmon resonance spectroscopy
© Springer International Publishing Switzerland 2014. The DNA hybridization of methicillin-resistant staphylococcus aureus (MRSA) was investigated by using surface plasmon resonance (SPR). 3 dimensional (3D) surface, carboxymethyl dextran hydrogel, were used as the sensor surface. The surface was fu...
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th-mahidol.335962018-11-09T09:17:40Z Label-free detection of meca-mediated methicillin-resistant staphylococcus aureus (MRSA) using surface plasmon resonance spectroscopy K. Nawattanapaiboon W. Kiatpathomchai P. Santanirund A. Wongsakulyanon B. Sutapun T. Srikhirin Mahidol University Thailand National Center for Genetic Engineering and Biotechnology Suranaree University of Technology Chemical Engineering Engineering © Springer International Publishing Switzerland 2014. The DNA hybridization of methicillin-resistant staphylococcus aureus (MRSA) was investigated by using surface plasmon resonance (SPR). 3 dimensional (3D) surface, carboxymethyl dextran hydrogel, were used as the sensor surface. The surface was functionalized with streptavidin where the biotinylated DNA probe can be specifically bound. Loop-mediated isothermal amplification (LAMP) products of MRSA were hybridized with the biotinylated probe and the biding was followed by SPR. The real time monitoring of hybridization kinetics of unlabeled mecA Lamp amplicons product was successfully carried out. The optimal condition of the probe density was 0.1 μM for MRSA at 0.06 nM. Non-specific absorption was not observed. 2018-11-09T02:04:05Z 2018-11-09T02:04:05Z 2014-01-01 Conference Paper IFMBE Proceedings. Vol.43, (2014), 845-848 10.1007/978-3-319-02913-9_218 16800737 2-s2.0-84922325291 https://repository.li.mahidol.ac.th/handle/123456789/33596 Mahidol University SCOPUS https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84922325291&origin=inward |
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Chemical Engineering Engineering K. Nawattanapaiboon W. Kiatpathomchai P. Santanirund A. Wongsakulyanon B. Sutapun T. Srikhirin Label-free detection of meca-mediated methicillin-resistant staphylococcus aureus (MRSA) using surface plasmon resonance spectroscopy |
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© Springer International Publishing Switzerland 2014. The DNA hybridization of methicillin-resistant staphylococcus aureus (MRSA) was investigated by using surface plasmon resonance (SPR). 3 dimensional (3D) surface, carboxymethyl dextran hydrogel, were used as the sensor surface. The surface was functionalized with streptavidin where the biotinylated DNA probe can be specifically bound. Loop-mediated isothermal amplification (LAMP) products of MRSA were hybridized with the biotinylated probe and the biding was followed by SPR. The real time monitoring of hybridization kinetics of unlabeled mecA Lamp amplicons product was successfully carried out. The optimal condition of the probe density was 0.1 μM for MRSA at 0.06 nM. Non-specific absorption was not observed. |
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Mahidol University |
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Mahidol University K. Nawattanapaiboon W. Kiatpathomchai P. Santanirund A. Wongsakulyanon B. Sutapun T. Srikhirin |
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Conference or Workshop Item |
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K. Nawattanapaiboon W. Kiatpathomchai P. Santanirund A. Wongsakulyanon B. Sutapun T. Srikhirin |
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K. Nawattanapaiboon |
title |
Label-free detection of meca-mediated methicillin-resistant staphylococcus aureus (MRSA) using surface plasmon resonance spectroscopy |
title_short |
Label-free detection of meca-mediated methicillin-resistant staphylococcus aureus (MRSA) using surface plasmon resonance spectroscopy |
title_full |
Label-free detection of meca-mediated methicillin-resistant staphylococcus aureus (MRSA) using surface plasmon resonance spectroscopy |
title_fullStr |
Label-free detection of meca-mediated methicillin-resistant staphylococcus aureus (MRSA) using surface plasmon resonance spectroscopy |
title_full_unstemmed |
Label-free detection of meca-mediated methicillin-resistant staphylococcus aureus (MRSA) using surface plasmon resonance spectroscopy |
title_sort |
label-free detection of meca-mediated methicillin-resistant staphylococcus aureus (mrsa) using surface plasmon resonance spectroscopy |
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2018 |
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https://repository.li.mahidol.ac.th/handle/123456789/33596 |
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1763489172135346176 |