Nested PCR detection of malaria directly using blood filter paper samples from epidemiological surveys

• Main Authors: Peipei Li, Zhenjun Zhao, Ying Wang, Hua Xing, Daniel M. Parker, Zhaoqing Yang, Elizabeth Baum, Wenli Li, Jetsumon Sattabongkot, Jeeraphat Sirichaisinthop, Shuying Li, Guiyun Yan, Liwang Cui, Qi Fan
• Other Authors: Dalian Institute of Biotechnology
• Format: Article
• Published: 2018
• Subjects: Immunology and Microbiology; Medicine
• Online Access: https://repository.li.mahidol.ac.th/handle/123456789/33970
• Institution: Mahidol University

Background: Nested PCR is considered a sensitive and specific method for detecting malaria parasites and is especially useful in epidemiological surveys. However, the preparation of DNA templates for PCR is often time-consuming and costly. Methods. A simplified PCR method was developed to directly u...