Production and characterization of a monoclonal antibody against recombinant cathepsin L1 of Fasciola gigantica

• Main Authors: Panat Anuracpreeda, Thippawan Srirakam, Sudarat Pandonlan, Narin Changklungmoa, Charoonroj Chotwiwatthanakun, Yotsawan Tinikul, Jaruwan Poljaroen, Krai Meemon, Prasert Sobhon
• Other Authors: Mahidol University
• Format: Article
• Published: 2018
• Subjects: Immunology and Microbiology; Medicine
• Online Access: https://repository.li.mahidol.ac.th/handle/123456789/34009

Monoclonal antibodies (MoAbs) against a recombinant cathepsin L1 of Fasciola gigantica (rFgCatL1) were produced in vitro by fusion of BALB/c mice spleen cells immunized with rFgCatL1 and mouse myeloma cells. Reactivity and specificity of these MoAbs were evaluated by indirect ELISA and immunoblottin...