Evaluation of nested PCR technique for detection of Pythium insidiosum in pathological specimens from patients with suspected fungal keratitis

• Main Authors: Panida Kosrirukvongs, Angkana Chaiprasert, Mongkol Uiprasertkul, Metinee Chongcharoen, Ramrada Banyong, Theerapong Krajaejun, Wanchai Wanachiwanawin
• Other Authors: Mahidol University
• Format: Article
• Published: 2018
• Subjects: Medicine
• Online Access: https://repository.li.mahidol.ac.th/handle/123456789/34394

Diagnosis of Pythium keratitis is problematic due to the difficulty in obtaining a culture report resulting in unnecessarily prolonged usage of antimicrobial medication due to misdiagnosis. This study evaluated and compared nested PCR technique with culture and immunoperoxidase staining assays of Py...