Suppression of α-glucosidase gene by RNA interference can increase resistance of Culex quinquefasciatus to Bacillus sphaericus binary toxin
© 2015 Taylor & Francis. Bacillus sphaericus binary toxin displays a specific toxicity towards Culex spp. larvae. Resistance of Culex quinquefasciatus to B. sphaericus is associated with the reduction or absence of 66-kDa α-glucosidase, a binary toxin receptor, in larval midgut microvilli. In...
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th-mahidol.350542018-11-23T16:28:16Z Suppression of α-glucosidase gene by RNA interference can increase resistance of Culex quinquefasciatus to Bacillus sphaericus binary toxin Warunya Woradulayapinij Mongkon Audtho Chanpen Wiwat Mahidol University Thailand National Center for Genetic Engineering and Biotechnology Agricultural and Biological Sciences © 2015 Taylor & Francis. Bacillus sphaericus binary toxin displays a specific toxicity towards Culex spp. larvae. Resistance of Culex quinquefasciatus to B. sphaericus is associated with the reduction or absence of 66-kDa α-glucosidase, a binary toxin receptor, in larval midgut microvilli. In this study, long double-stranded RNA (dsRNA) targeting the α-glucosidase gene was used to suppress the expression of α-glucosidase in Cx. quinquefasciatus larvae and the consequences of the suppression was investigated. Three sets of dsRNAs, dsGRi1, dsGRi2, and dsGRi3, which are homologous to distinct regions of α-glucosidase gene, were delivered to mosquito larva by feeding with dsRNA–chitosan particles. Reverse transcriptase-polymerase chain reaction results demonstrated that the expression of α-glucosidase mRNA was reduced by 43% in larvae fed with dsGRi3–chitosan, compared to those fed with dsgfp–chitosan particles. Western blot analysis revealed a 7–17% reduction of α-glucosidase protein expression in larvae fed with dsGRi–chitosan. Mortality rates of larvae that received dsRNAs–chitosan particles were only 45–62% compared to those that received dsgfp–chitosan particles. These results indicate that α-glucosidase is essential for the toxicity of B. sphaericus binary toxin to Cx. quinquefasciatus. 2018-11-23T09:28:16Z 2018-11-23T09:28:16Z 2015-12-02 Article Biocontrol Science and Technology. Vol.25, No.12 (2015), 1453-1466 10.1080/09583157.2015.1066310 13600478 09583157 2-s2.0-84944059456 https://repository.li.mahidol.ac.th/handle/123456789/35054 Mahidol University SCOPUS https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84944059456&origin=inward |
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Agricultural and Biological Sciences Warunya Woradulayapinij Mongkon Audtho Chanpen Wiwat Suppression of α-glucosidase gene by RNA interference can increase resistance of Culex quinquefasciatus to Bacillus sphaericus binary toxin |
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© 2015 Taylor & Francis. Bacillus sphaericus binary toxin displays a specific toxicity towards Culex spp. larvae. Resistance of Culex quinquefasciatus to B. sphaericus is associated with the reduction or absence of 66-kDa α-glucosidase, a binary toxin receptor, in larval midgut microvilli. In this study, long double-stranded RNA (dsRNA) targeting the α-glucosidase gene was used to suppress the expression of α-glucosidase in Cx. quinquefasciatus larvae and the consequences of the suppression was investigated. Three sets of dsRNAs, dsGRi1, dsGRi2, and dsGRi3, which are homologous to distinct regions of α-glucosidase gene, were delivered to mosquito larva by feeding with dsRNA–chitosan particles. Reverse transcriptase-polymerase chain reaction results demonstrated that the expression of α-glucosidase mRNA was reduced by 43% in larvae fed with dsGRi3–chitosan, compared to those fed with dsgfp–chitosan particles. Western blot analysis revealed a 7–17% reduction of α-glucosidase protein expression in larvae fed with dsGRi–chitosan. Mortality rates of larvae that received dsRNAs–chitosan particles were only 45–62% compared to those that received dsgfp–chitosan particles. These results indicate that α-glucosidase is essential for the toxicity of B. sphaericus binary toxin to Cx. quinquefasciatus. |
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Mahidol University Warunya Woradulayapinij Mongkon Audtho Chanpen Wiwat |
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Warunya Woradulayapinij Mongkon Audtho Chanpen Wiwat |
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Warunya Woradulayapinij |
title |
Suppression of α-glucosidase gene by RNA interference can increase resistance of Culex quinquefasciatus to Bacillus sphaericus binary toxin |
title_short |
Suppression of α-glucosidase gene by RNA interference can increase resistance of Culex quinquefasciatus to Bacillus sphaericus binary toxin |
title_full |
Suppression of α-glucosidase gene by RNA interference can increase resistance of Culex quinquefasciatus to Bacillus sphaericus binary toxin |
title_fullStr |
Suppression of α-glucosidase gene by RNA interference can increase resistance of Culex quinquefasciatus to Bacillus sphaericus binary toxin |
title_full_unstemmed |
Suppression of α-glucosidase gene by RNA interference can increase resistance of Culex quinquefasciatus to Bacillus sphaericus binary toxin |
title_sort |
suppression of α-glucosidase gene by rna interference can increase resistance of culex quinquefasciatus to bacillus sphaericus binary toxin |
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2018 |
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https://repository.li.mahidol.ac.th/handle/123456789/35054 |
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1763492487129726976 |