Characterization of plasmodium vivax early transcribed membrane protein 11.2 and exported protein 1
© 2015 Cheng et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. In Plasmodium, the membrane of intracellular...
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th-mahidol.351712018-11-23T16:43:32Z Characterization of plasmodium vivax early transcribed membrane protein 11.2 and exported protein 1 Yang Cheng Feng Lu Seong Kyun Lee Deok Hoon Kong Kwon Soo Ha Bo Wang Jetsumon Sattabongkot Takafumi Tsuboi Eun Taek Han Kangwon National University National Institute of Allergy and Infectious Diseases Jiangsu Institute of Parasitic Diseases Anhui Medical University Mahidol University Ehime University Agricultural and Biological Sciences Biochemistry, Genetics and Molecular Biology © 2015 Cheng et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. In Plasmodium, the membrane of intracellular parasites is initially formed during invasion as an invagination of the red blood cell surface, which forms a barrier between the parasite and infected red blood cells in asexual blood stage parasites. The membrane proteins of intracellular parasites of Plasmodium species have been identified such as early-transcribed membrane proteins (ETRAMPs) and exported proteins (EXPs). However, there is little or no information regarding the intracellular parasite membrane in Plasmodium vivax. In the present study, recombinant PvETRAMP11.2 (PVX-003565) and PvEXP1 (PVX-091700) were expressed and evaluated antigenicity tests using sera from P. vivax-infected patients. A large proportion of infected individuals presented with IgG antibody responses against PvETRAMP11.2 (76.8%) and PvEXP1 (69.6%). Both of the recombinant proteins elicited high antibody titers capable of recognizing parasites of vivax malaria patients. PvETRAMP11.2 partially co-localized with PvEXP1 on the intracellular membranes of immature schizont. Moreover, they were also detected at the apical organelles of newly formed merozoites of mature schizont. We first proposed that these proteins might be synthesized in the preceding schizont stage, localized on the parasite membranes and apical organelles of infected erythrocytes, and induced high IgG antibody responses in patients. 2018-11-23T09:31:19Z 2018-11-23T09:31:19Z 2015-05-01 Article PLoS ONE. Vol.10, No.5 (2015) 10.1371/journal.pone.0127500 19326203 2-s2.0-84960145752 https://repository.li.mahidol.ac.th/handle/123456789/35171 Mahidol University SCOPUS https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84960145752&origin=inward |
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Agricultural and Biological Sciences Biochemistry, Genetics and Molecular Biology Yang Cheng Feng Lu Seong Kyun Lee Deok Hoon Kong Kwon Soo Ha Bo Wang Jetsumon Sattabongkot Takafumi Tsuboi Eun Taek Han Characterization of plasmodium vivax early transcribed membrane protein 11.2 and exported protein 1 |
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© 2015 Cheng et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. In Plasmodium, the membrane of intracellular parasites is initially formed during invasion as an invagination of the red blood cell surface, which forms a barrier between the parasite and infected red blood cells in asexual blood stage parasites. The membrane proteins of intracellular parasites of Plasmodium species have been identified such as early-transcribed membrane proteins (ETRAMPs) and exported proteins (EXPs). However, there is little or no information regarding the intracellular parasite membrane in Plasmodium vivax. In the present study, recombinant PvETRAMP11.2 (PVX-003565) and PvEXP1 (PVX-091700) were expressed and evaluated antigenicity tests using sera from P. vivax-infected patients. A large proportion of infected individuals presented with IgG antibody responses against PvETRAMP11.2 (76.8%) and PvEXP1 (69.6%). Both of the recombinant proteins elicited high antibody titers capable of recognizing parasites of vivax malaria patients. PvETRAMP11.2 partially co-localized with PvEXP1 on the intracellular membranes of immature schizont. Moreover, they were also detected at the apical organelles of newly formed merozoites of mature schizont. We first proposed that these proteins might be synthesized in the preceding schizont stage, localized on the parasite membranes and apical organelles of infected erythrocytes, and induced high IgG antibody responses in patients. |
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Kangwon National University |
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Kangwon National University Yang Cheng Feng Lu Seong Kyun Lee Deok Hoon Kong Kwon Soo Ha Bo Wang Jetsumon Sattabongkot Takafumi Tsuboi Eun Taek Han |
format |
Article |
author |
Yang Cheng Feng Lu Seong Kyun Lee Deok Hoon Kong Kwon Soo Ha Bo Wang Jetsumon Sattabongkot Takafumi Tsuboi Eun Taek Han |
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Yang Cheng |
title |
Characterization of plasmodium vivax early transcribed membrane protein 11.2 and exported protein 1 |
title_short |
Characterization of plasmodium vivax early transcribed membrane protein 11.2 and exported protein 1 |
title_full |
Characterization of plasmodium vivax early transcribed membrane protein 11.2 and exported protein 1 |
title_fullStr |
Characterization of plasmodium vivax early transcribed membrane protein 11.2 and exported protein 1 |
title_full_unstemmed |
Characterization of plasmodium vivax early transcribed membrane protein 11.2 and exported protein 1 |
title_sort |
characterization of plasmodium vivax early transcribed membrane protein 11.2 and exported protein 1 |
publishDate |
2018 |
url |
https://repository.li.mahidol.ac.th/handle/123456789/35171 |
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1763497183397543936 |