Suppression of shrimp melanization during white spot syndrome virus infection
© 2015 by The American Society for Biochemistry and Molecular Biology, Inc. The melanization cascade, activated by the prophenoloxidase (proPO) system, plays a key role in the production of cytotoxic intermediates, as well as melanin products for microbial sequestration in invertebrates. Here, we sh...
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th-mahidol.354882018-11-23T16:45:18Z Suppression of shrimp melanization during white spot syndrome virus infection Jantiwan Sutthangkul Piti Amparyup Walaiporn Charoensapsri Saengchan Senapin Kornsunee Phiwsaiya Anchalee Tassanakajon Chulalongkorn University Thailand National Center for Genetic Engineering and Biotechnology Mahidol University Biochemistry, Genetics and Molecular Biology © 2015 by The American Society for Biochemistry and Molecular Biology, Inc. The melanization cascade, activated by the prophenoloxidase (proPO) system, plays a key role in the production of cytotoxic intermediates, as well as melanin products for microbial sequestration in invertebrates. Here, we show that the proPO system is an important component of the Penaeus monodon shrimp immune defense toward a major viral pathogen, white spot syndrome virus (WSSV). Gene silencing of PmproPO(s) resulted in increased cumulative shrimp mortality after WSSV infection, whereas incubation ofWSSVwith an in vitro melanization reaction prior to injection into shrimp significantly increased the shrimp survival rate. The hemolymph phenoloxidase (PO) activity of WSSV-infected shrimp was extremely reduced at days 2 and 3 post-injection compared with uninfected shrimp but was fully restored after the addition of exogenous trypsin, suggesting that WSSV probably inhibits the activity of some proteinases in the proPO cascade. Using yeast two-hybrid screening and co-immunoprecipitation assays, the viral protein WSSV453 was found to interact with the proPO-activating enzyme 2 (PmPPAE2) of P. monodon. Gene silencing of WSSV453 showed a significant increase of PO activity in WSSV-infected shrimp, whereas co-silencing of WSSV453 and PmPPAE2 did not, suggesting that silencing of WSSV453 partially restored the PO activity via PmPPAE2 in WSSV-infected shrimp. Moreover, the activation of PO activity in shrimp plasma by PmPPAE2 was significantly decreased by preincubation with recombinant WSSV453. These results suggest that the inhibition of the shrimp proPO system byWSSVpartly occurs via thePmPPAE2-inhibiting activity of WSSV453. 2018-11-23T09:45:18Z 2018-11-23T09:45:18Z 2015-03-06 Article Journal of Biological Chemistry. Vol.290, No.10 (2015), 6470-6481 10.1074/jbc.M114.605568 1083351X 00219258 2-s2.0-84924891305 https://repository.li.mahidol.ac.th/handle/123456789/35488 Mahidol University SCOPUS https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84924891305&origin=inward |
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Biochemistry, Genetics and Molecular Biology Jantiwan Sutthangkul Piti Amparyup Walaiporn Charoensapsri Saengchan Senapin Kornsunee Phiwsaiya Anchalee Tassanakajon Suppression of shrimp melanization during white spot syndrome virus infection |
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© 2015 by The American Society for Biochemistry and Molecular Biology, Inc. The melanization cascade, activated by the prophenoloxidase (proPO) system, plays a key role in the production of cytotoxic intermediates, as well as melanin products for microbial sequestration in invertebrates. Here, we show that the proPO system is an important component of the Penaeus monodon shrimp immune defense toward a major viral pathogen, white spot syndrome virus (WSSV). Gene silencing of PmproPO(s) resulted in increased cumulative shrimp mortality after WSSV infection, whereas incubation ofWSSVwith an in vitro melanization reaction prior to injection into shrimp significantly increased the shrimp survival rate. The hemolymph phenoloxidase (PO) activity of WSSV-infected shrimp was extremely reduced at days 2 and 3 post-injection compared with uninfected shrimp but was fully restored after the addition of exogenous trypsin, suggesting that WSSV probably inhibits the activity of some proteinases in the proPO cascade. Using yeast two-hybrid screening and co-immunoprecipitation assays, the viral protein WSSV453 was found to interact with the proPO-activating enzyme 2 (PmPPAE2) of P. monodon. Gene silencing of WSSV453 showed a significant increase of PO activity in WSSV-infected shrimp, whereas co-silencing of WSSV453 and PmPPAE2 did not, suggesting that silencing of WSSV453 partially restored the PO activity via PmPPAE2 in WSSV-infected shrimp. Moreover, the activation of PO activity in shrimp plasma by PmPPAE2 was significantly decreased by preincubation with recombinant WSSV453. These results suggest that the inhibition of the shrimp proPO system byWSSVpartly occurs via thePmPPAE2-inhibiting activity of WSSV453. |
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Chulalongkorn University |
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Chulalongkorn University Jantiwan Sutthangkul Piti Amparyup Walaiporn Charoensapsri Saengchan Senapin Kornsunee Phiwsaiya Anchalee Tassanakajon |
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Article |
author |
Jantiwan Sutthangkul Piti Amparyup Walaiporn Charoensapsri Saengchan Senapin Kornsunee Phiwsaiya Anchalee Tassanakajon |
author_sort |
Jantiwan Sutthangkul |
title |
Suppression of shrimp melanization during white spot syndrome virus infection |
title_short |
Suppression of shrimp melanization during white spot syndrome virus infection |
title_full |
Suppression of shrimp melanization during white spot syndrome virus infection |
title_fullStr |
Suppression of shrimp melanization during white spot syndrome virus infection |
title_full_unstemmed |
Suppression of shrimp melanization during white spot syndrome virus infection |
title_sort |
suppression of shrimp melanization during white spot syndrome virus infection |
publishDate |
2018 |
url |
https://repository.li.mahidol.ac.th/handle/123456789/35488 |
_version_ |
1763491147164942336 |