Plasmodium vivax: Restricted tropism and rapid remodeling of CD71-positive reticulocytes
© 2015 by The American Society of Hematology. Plasmodium vivax merozoites only invade reticulocytes, a minor though heterogeneous population of red blood cell precursors that can be graded by levels of transferrin receptor (CD71) expression. The development of a protocol that allows sorting reticulo...
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th-mahidol.354992018-11-23T17:49:51Z Plasmodium vivax: Restricted tropism and rapid remodeling of CD71-positive reticulocytes Benoit Malleret Ang Li Rou Zhang Kevin S.W. Tan Rossarin Suwanarusk Carla Claser Jee Sun Cho Esther Geok Liang Koh Cindy S. Chu Sasithon Pukrittayakamee Mah Lee Ng Florent Ginhoux Lai Guan Ng Chwee Teck Lim François Nosten Georges Snounou Laurent Rénia Bruce Russell Yong Loo Lin School of Medicine Agency for Science, Technology and Research, Singapore Mechanobiology Institute, Singapore National University of Singapore Mahidol University Nuffield Department of Clinical Medicine Sorbonne Universite CNRS Centre National de la Recherche Scientifique Biochemistry, Genetics and Molecular Biology Immunology and Microbiology Medicine © 2015 by The American Society of Hematology. Plasmodium vivax merozoites only invade reticulocytes, a minor though heterogeneous population of red blood cell precursors that can be graded by levels of transferrin receptor (CD71) expression. The development of a protocol that allows sorting reticulocytes into defined developmental stages and a robust ex vivo P vivax invasion assay has made it possible for the first time to investigate the fine-scale invasion preference of P vivax merozoites. Surprisingly, it was the immature reticulocytes (CD71+) that are generally restricted to the bone marrow that were preferentially invaded, whereas older reticulocytes (CD71-), principally found in the peripheral blood, were rarely invaded. Invasion assays based on the CD71+reticulocyte fraction revealed substantial postinvasion modification. Thus, 3 to 6 hours after invasion, the initially biomechanically rigid CD71+reticulocytes convert into a highly deformable CD71-infected red blood cell devoid of host reticular matter, a process that normally spans 24 hours for uninfected reticulocytes. Concurrent with these changes, clathrin pits disappear by 3 hours postinvasion, replaced by distinctive caveolae nanostructures. These 2 hitherto unsuspected features of P vivax invasion, a narrow preference for immature reticulocytes and a rapid remodeling of the host cell, provide important insights pertinent to the pathobiology of the P vivax infection. 2018-11-23T09:45:43Z 2018-11-23T09:45:43Z 2015-02-19 Article Blood. Vol.125, No.8 (2015), 1314-1324 10.1182/blood-2014-08-596015 15280020 00064971 2-s2.0-84923351850 https://repository.li.mahidol.ac.th/handle/123456789/35499 Mahidol University SCOPUS https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84923351850&origin=inward |
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Biochemistry, Genetics and Molecular Biology Immunology and Microbiology Medicine Benoit Malleret Ang Li Rou Zhang Kevin S.W. Tan Rossarin Suwanarusk Carla Claser Jee Sun Cho Esther Geok Liang Koh Cindy S. Chu Sasithon Pukrittayakamee Mah Lee Ng Florent Ginhoux Lai Guan Ng Chwee Teck Lim François Nosten Georges Snounou Laurent Rénia Bruce Russell Plasmodium vivax: Restricted tropism and rapid remodeling of CD71-positive reticulocytes |
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© 2015 by The American Society of Hematology. Plasmodium vivax merozoites only invade reticulocytes, a minor though heterogeneous population of red blood cell precursors that can be graded by levels of transferrin receptor (CD71) expression. The development of a protocol that allows sorting reticulocytes into defined developmental stages and a robust ex vivo P vivax invasion assay has made it possible for the first time to investigate the fine-scale invasion preference of P vivax merozoites. Surprisingly, it was the immature reticulocytes (CD71+) that are generally restricted to the bone marrow that were preferentially invaded, whereas older reticulocytes (CD71-), principally found in the peripheral blood, were rarely invaded. Invasion assays based on the CD71+reticulocyte fraction revealed substantial postinvasion modification. Thus, 3 to 6 hours after invasion, the initially biomechanically rigid CD71+reticulocytes convert into a highly deformable CD71-infected red blood cell devoid of host reticular matter, a process that normally spans 24 hours for uninfected reticulocytes. Concurrent with these changes, clathrin pits disappear by 3 hours postinvasion, replaced by distinctive caveolae nanostructures. These 2 hitherto unsuspected features of P vivax invasion, a narrow preference for immature reticulocytes and a rapid remodeling of the host cell, provide important insights pertinent to the pathobiology of the P vivax infection. |
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Yong Loo Lin School of Medicine |
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Yong Loo Lin School of Medicine Benoit Malleret Ang Li Rou Zhang Kevin S.W. Tan Rossarin Suwanarusk Carla Claser Jee Sun Cho Esther Geok Liang Koh Cindy S. Chu Sasithon Pukrittayakamee Mah Lee Ng Florent Ginhoux Lai Guan Ng Chwee Teck Lim François Nosten Georges Snounou Laurent Rénia Bruce Russell |
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Article |
author |
Benoit Malleret Ang Li Rou Zhang Kevin S.W. Tan Rossarin Suwanarusk Carla Claser Jee Sun Cho Esther Geok Liang Koh Cindy S. Chu Sasithon Pukrittayakamee Mah Lee Ng Florent Ginhoux Lai Guan Ng Chwee Teck Lim François Nosten Georges Snounou Laurent Rénia Bruce Russell |
author_sort |
Benoit Malleret |
title |
Plasmodium vivax: Restricted tropism and rapid remodeling of CD71-positive reticulocytes |
title_short |
Plasmodium vivax: Restricted tropism and rapid remodeling of CD71-positive reticulocytes |
title_full |
Plasmodium vivax: Restricted tropism and rapid remodeling of CD71-positive reticulocytes |
title_fullStr |
Plasmodium vivax: Restricted tropism and rapid remodeling of CD71-positive reticulocytes |
title_full_unstemmed |
Plasmodium vivax: Restricted tropism and rapid remodeling of CD71-positive reticulocytes |
title_sort |
plasmodium vivax: restricted tropism and rapid remodeling of cd71-positive reticulocytes |
publishDate |
2018 |
url |
https://repository.li.mahidol.ac.th/handle/123456789/35499 |
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1763490156834193408 |