Secondhand smoke exposure-induced nucleocytoplasmic shuttling of HMGB1 in a rat premature skin aging model

© 2014 The Authors. Published by Elsevier Inc. Secondhand cigarette smoke exposure (SSE) has been linked to carcinogenic, oxidative, and inflammatory reactions. Herein, we investigated whether premature skin aging could be induced by SSE in a rat model, and assessed the cytoplasmic translocation of...

Full description

Saved in:
Bibliographic Details
Main Authors: Sirintip Chaichalotornkul, Thamthiwat Nararatwanchai, Somphong Narkpinit, Pornpen Dararat, Kiyoshi Kikuchi, Ikuro Maruyama, Salunya Tancharoen
Other Authors: Mae Fah Luang University
Format: Article
Published: 2018
Subjects:
Online Access:https://repository.li.mahidol.ac.th/handle/123456789/35509
Tags: Add Tag
No Tags, Be the first to tag this record!
Institution: Mahidol University
id th-mahidol.35509
record_format dspace
spelling th-mahidol.355092018-11-23T16:46:21Z Secondhand smoke exposure-induced nucleocytoplasmic shuttling of HMGB1 in a rat premature skin aging model Sirintip Chaichalotornkul Thamthiwat Nararatwanchai Somphong Narkpinit Pornpen Dararat Kiyoshi Kikuchi Ikuro Maruyama Salunya Tancharoen Mae Fah Luang University Mahidol University Kurume University School of Medicine Kagoshima University Faculty of Medicine Biochemistry, Genetics and Molecular Biology © 2014 The Authors. Published by Elsevier Inc. Secondhand cigarette smoke exposure (SSE) has been linked to carcinogenic, oxidative, and inflammatory reactions. Herein, we investigated whether premature skin aging could be induced by SSE in a rat model, and assessed the cytoplasmic translocation of high mobility group box 1 (HMGB1) protein and collagen loss in skin tissues. Animals were divided into two groups: SSE and controls. Whole body SSE was carried out for 12 weeks. Dorsal skin tissue specimens were harvested for HMGB1 and Mallory's azan staining. Correlations between serum HMGB1 and collagen levels were determined. Rat skin exposed to secondhand smoke lost collagen bundles in the papillary dermis and collagen decreased significantly (p < 0.05) compared with control rats. In epidermal keratinocytes, cytoplasmic HMGB1 staining was more diffuse and there were more HMGB1-positive cells after four weeks in SSE compared to control rats. A negative correlation between HMGB1 serum and collagen levels (r = -0.631, p = 0.28) was also observed. Therefore, cytoplasmic HMGB1 expression in skin tissues might be associated with skin collagen loss upon the initiation of SSE. Additionally, long-term SSE might affect the appearance of the skin, or could accelerate the skin aging process. 2018-11-23T09:46:21Z 2018-11-23T09:46:21Z 2015-01-02 Article Biochemical and Biophysical Research Communications. Vol.456, No.1 (2015), 92-97 10.1016/j.bbrc.2014.11.040 10902104 0006291X 2-s2.0-84919607898 https://repository.li.mahidol.ac.th/handle/123456789/35509 Mahidol University SCOPUS https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84919607898&origin=inward
institution Mahidol University
building Mahidol University Library
continent Asia
country Thailand
Thailand
content_provider Mahidol University Library
collection Mahidol University Institutional Repository
topic Biochemistry, Genetics and Molecular Biology
spellingShingle Biochemistry, Genetics and Molecular Biology
Sirintip Chaichalotornkul
Thamthiwat Nararatwanchai
Somphong Narkpinit
Pornpen Dararat
Kiyoshi Kikuchi
Ikuro Maruyama
Salunya Tancharoen
Secondhand smoke exposure-induced nucleocytoplasmic shuttling of HMGB1 in a rat premature skin aging model
description © 2014 The Authors. Published by Elsevier Inc. Secondhand cigarette smoke exposure (SSE) has been linked to carcinogenic, oxidative, and inflammatory reactions. Herein, we investigated whether premature skin aging could be induced by SSE in a rat model, and assessed the cytoplasmic translocation of high mobility group box 1 (HMGB1) protein and collagen loss in skin tissues. Animals were divided into two groups: SSE and controls. Whole body SSE was carried out for 12 weeks. Dorsal skin tissue specimens were harvested for HMGB1 and Mallory's azan staining. Correlations between serum HMGB1 and collagen levels were determined. Rat skin exposed to secondhand smoke lost collagen bundles in the papillary dermis and collagen decreased significantly (p < 0.05) compared with control rats. In epidermal keratinocytes, cytoplasmic HMGB1 staining was more diffuse and there were more HMGB1-positive cells after four weeks in SSE compared to control rats. A negative correlation between HMGB1 serum and collagen levels (r = -0.631, p = 0.28) was also observed. Therefore, cytoplasmic HMGB1 expression in skin tissues might be associated with skin collagen loss upon the initiation of SSE. Additionally, long-term SSE might affect the appearance of the skin, or could accelerate the skin aging process.
author2 Mae Fah Luang University
author_facet Mae Fah Luang University
Sirintip Chaichalotornkul
Thamthiwat Nararatwanchai
Somphong Narkpinit
Pornpen Dararat
Kiyoshi Kikuchi
Ikuro Maruyama
Salunya Tancharoen
format Article
author Sirintip Chaichalotornkul
Thamthiwat Nararatwanchai
Somphong Narkpinit
Pornpen Dararat
Kiyoshi Kikuchi
Ikuro Maruyama
Salunya Tancharoen
author_sort Sirintip Chaichalotornkul
title Secondhand smoke exposure-induced nucleocytoplasmic shuttling of HMGB1 in a rat premature skin aging model
title_short Secondhand smoke exposure-induced nucleocytoplasmic shuttling of HMGB1 in a rat premature skin aging model
title_full Secondhand smoke exposure-induced nucleocytoplasmic shuttling of HMGB1 in a rat premature skin aging model
title_fullStr Secondhand smoke exposure-induced nucleocytoplasmic shuttling of HMGB1 in a rat premature skin aging model
title_full_unstemmed Secondhand smoke exposure-induced nucleocytoplasmic shuttling of HMGB1 in a rat premature skin aging model
title_sort secondhand smoke exposure-induced nucleocytoplasmic shuttling of hmgb1 in a rat premature skin aging model
publishDate 2018
url https://repository.li.mahidol.ac.th/handle/123456789/35509
_version_ 1763493046109863936