Missense mutations in exons 18-24 of EGFR in hepatocellular carcinoma tissues
© 2015 Ravat Panvichian et al. Epidermal growth factor receptor (EGFR), a transmembrane tyrosine kinase receptor, plays important roles in various cancers. In nonsmall cell lung cancer (NSCLC), EGFR mutations cluster around the ATP-binding pocket (exons 18-21) and some of these mutations activate th...
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th-mahidol.355672018-11-23T17:19:47Z Missense mutations in exons 18-24 of EGFR in hepatocellular carcinoma tissues Ravat Panvichian Anchalee Tantiwetrueangdet Pattana Sornmayura Surasak Leelaudomlipi Mahidol University Biochemistry, Genetics and Molecular Biology Immunology and Microbiology © 2015 Ravat Panvichian et al. Epidermal growth factor receptor (EGFR), a transmembrane tyrosine kinase receptor, plays important roles in various cancers. In nonsmall cell lung cancer (NSCLC), EGFR mutations cluster around the ATP-binding pocket (exons 18-21) and some of these mutations activate the kinase and induce an increased sensitivity to EGFR-tyrosine kinase inhibitors. Nevertheless, data of EGFR mutations in HCC are limited. In this study, we investigated EGFR expression by immunohistochemistry and EGFR mutations (exons 18-24) by PCR cloning and sequencing. EGFR overexpression in HCC and matched nontumor tissues were detected in 13/40 (32.5%) and 10/35 (28.6%), respectively. Moreover, missense and silent mutations were detected in 13/33 (39.4%) and 11/33 (33.3%) of HCC tissues, respectively. The thirteen different missense mutations were p.L730P, p.V742I, p.K757E, p.I780T, p.N808S, p.R831C, p.V851A, p.V897A, p.S912P, p.P937L, p.T940A, p.M947V, and p.M947T. We also found already known SNP, p.Q787Q (CAG>CAA), in 13/33 (39.4%) of HCC tissues. However, no significant association was detected between EGFR mutations and EGFR overexpression, tissue, age, sex, tumor size, AFP, HBsAg, TP53, and Ki-67. Further investigation is warranted to validate the frequency and activity of these missense mutations, as well as their roles in HCC tumorigenesis and in EGFR-targeted therapy. 2018-11-23T09:48:37Z 2018-11-23T09:48:37Z 2015-01-01 Article BioMed Research International. Vol.2015, (2015) 10.1155/2015/171845 23146141 23146133 2-s2.0-84942246895 https://repository.li.mahidol.ac.th/handle/123456789/35567 Mahidol University SCOPUS https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84942246895&origin=inward |
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Biochemistry, Genetics and Molecular Biology Immunology and Microbiology Ravat Panvichian Anchalee Tantiwetrueangdet Pattana Sornmayura Surasak Leelaudomlipi Missense mutations in exons 18-24 of EGFR in hepatocellular carcinoma tissues |
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© 2015 Ravat Panvichian et al. Epidermal growth factor receptor (EGFR), a transmembrane tyrosine kinase receptor, plays important roles in various cancers. In nonsmall cell lung cancer (NSCLC), EGFR mutations cluster around the ATP-binding pocket (exons 18-21) and some of these mutations activate the kinase and induce an increased sensitivity to EGFR-tyrosine kinase inhibitors. Nevertheless, data of EGFR mutations in HCC are limited. In this study, we investigated EGFR expression by immunohistochemistry and EGFR mutations (exons 18-24) by PCR cloning and sequencing. EGFR overexpression in HCC and matched nontumor tissues were detected in 13/40 (32.5%) and 10/35 (28.6%), respectively. Moreover, missense and silent mutations were detected in 13/33 (39.4%) and 11/33 (33.3%) of HCC tissues, respectively. The thirteen different missense mutations were p.L730P, p.V742I, p.K757E, p.I780T, p.N808S, p.R831C, p.V851A, p.V897A, p.S912P, p.P937L, p.T940A, p.M947V, and p.M947T. We also found already known SNP, p.Q787Q (CAG>CAA), in 13/33 (39.4%) of HCC tissues. However, no significant association was detected between EGFR mutations and EGFR overexpression, tissue, age, sex, tumor size, AFP, HBsAg, TP53, and Ki-67. Further investigation is warranted to validate the frequency and activity of these missense mutations, as well as their roles in HCC tumorigenesis and in EGFR-targeted therapy. |
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Mahidol University |
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Mahidol University Ravat Panvichian Anchalee Tantiwetrueangdet Pattana Sornmayura Surasak Leelaudomlipi |
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Article |
author |
Ravat Panvichian Anchalee Tantiwetrueangdet Pattana Sornmayura Surasak Leelaudomlipi |
author_sort |
Ravat Panvichian |
title |
Missense mutations in exons 18-24 of EGFR in hepatocellular carcinoma tissues |
title_short |
Missense mutations in exons 18-24 of EGFR in hepatocellular carcinoma tissues |
title_full |
Missense mutations in exons 18-24 of EGFR in hepatocellular carcinoma tissues |
title_fullStr |
Missense mutations in exons 18-24 of EGFR in hepatocellular carcinoma tissues |
title_full_unstemmed |
Missense mutations in exons 18-24 of EGFR in hepatocellular carcinoma tissues |
title_sort |
missense mutations in exons 18-24 of egfr in hepatocellular carcinoma tissues |
publishDate |
2018 |
url |
https://repository.li.mahidol.ac.th/handle/123456789/35567 |
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1763489349328961536 |