Evaluation of polysaccharide-based latex agglutination assays for the rapid detection of antibodies to burkholderia pseudomallei
Copyright © 2015 by The American Society of Tropical Medicine and Hygiene. Melioidosis is a severe disease caused by the Gram-negative bacterium Burkholderia pseudomallei. Diagnosis of melioidosis currently relies on the isolation of B. pseudomallei from clinical samples, which can take several days...
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th-mahidol.360792018-11-23T17:37:25Z Evaluation of polysaccharide-based latex agglutination assays for the rapid detection of antibodies to burkholderia pseudomallei Vichaya Suttisunhakul Narisara Chantratita Chanthiwa Wikraiphat Vanaporn Wuthiekanun Zakiya Douglas Nicholas P.J. Day Direk Limmathurotsakul Paul J. Brett Mary N. Burtnick Mahidol University Immunology and Microbiology Medicine Copyright © 2015 by The American Society of Tropical Medicine and Hygiene. Melioidosis is a severe disease caused by the Gram-negative bacterium Burkholderia pseudomallei. Diagnosis of melioidosis currently relies on the isolation of B. pseudomallei from clinical samples, which can take several days. An indirect hemagglutination assay (IHA) is widely used for serodiagnosis, but it has a short shelf life, is poorly standardized, and requires a viable bacteria culture performed in a biosafety level 3 (BSL-3) laboratory. To improve the diagnostic methods, we have developed two rapid latex agglutination tests based on purified B. pseudomallei O-polysaccharide (OPS) and capsular polysaccharide (CPS) antigens. The immunodiagnostic potential of these tests was evaluated using serum from culture-confirmed melioidosis patients (N = 143) and healthy donors from either endemic (N = 199) or nonendemic areas (N = 90). The sensitivity of the OPS-based latex agglutination assay (OPS-latex; 84.4%) was significantly higher than both the CPS-latex (69.5%) (P < 0.001) and IHA (69.5%) (P = 0.001). When evaluated with Thai donor serum, the OPS-latex had comparable specificity (56.9%) to the CPS-latex (63.8%) (P = 0.053), but was significantly lower than the IHA (67.6%) (P = 0.002). In contrast, all tests with U.S. donor serum were highly specific (≥ 97.8%). These results suggest that polysaccharide-based latex agglutination assays may be useful for serodiagnosis of melioidosis in non-endemic areas. 2018-11-23T10:15:58Z 2018-11-23T10:15:58Z 2015-09-01 Article American Journal of Tropical Medicine and Hygiene. Vol.93, No.3 (2015), 542-546 10.4269/ajtmh.15-0114 00029637 2-s2.0-84941628934 https://repository.li.mahidol.ac.th/handle/123456789/36079 Mahidol University SCOPUS https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84941628934&origin=inward |
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Immunology and Microbiology Medicine Vichaya Suttisunhakul Narisara Chantratita Chanthiwa Wikraiphat Vanaporn Wuthiekanun Zakiya Douglas Nicholas P.J. Day Direk Limmathurotsakul Paul J. Brett Mary N. Burtnick Evaluation of polysaccharide-based latex agglutination assays for the rapid detection of antibodies to burkholderia pseudomallei |
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Copyright © 2015 by The American Society of Tropical Medicine and Hygiene. Melioidosis is a severe disease caused by the Gram-negative bacterium Burkholderia pseudomallei. Diagnosis of melioidosis currently relies on the isolation of B. pseudomallei from clinical samples, which can take several days. An indirect hemagglutination assay (IHA) is widely used for serodiagnosis, but it has a short shelf life, is poorly standardized, and requires a viable bacteria culture performed in a biosafety level 3 (BSL-3) laboratory. To improve the diagnostic methods, we have developed two rapid latex agglutination tests based on purified B. pseudomallei O-polysaccharide (OPS) and capsular polysaccharide (CPS) antigens. The immunodiagnostic potential of these tests was evaluated using serum from culture-confirmed melioidosis patients (N = 143) and healthy donors from either endemic (N = 199) or nonendemic areas (N = 90). The sensitivity of the OPS-based latex agglutination assay (OPS-latex; 84.4%) was significantly higher than both the CPS-latex (69.5%) (P < 0.001) and IHA (69.5%) (P = 0.001). When evaluated with Thai donor serum, the OPS-latex had comparable specificity (56.9%) to the CPS-latex (63.8%) (P = 0.053), but was significantly lower than the IHA (67.6%) (P = 0.002). In contrast, all tests with U.S. donor serum were highly specific (≥ 97.8%). These results suggest that polysaccharide-based latex agglutination assays may be useful for serodiagnosis of melioidosis in non-endemic areas. |
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Mahidol University |
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Mahidol University Vichaya Suttisunhakul Narisara Chantratita Chanthiwa Wikraiphat Vanaporn Wuthiekanun Zakiya Douglas Nicholas P.J. Day Direk Limmathurotsakul Paul J. Brett Mary N. Burtnick |
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Article |
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Vichaya Suttisunhakul Narisara Chantratita Chanthiwa Wikraiphat Vanaporn Wuthiekanun Zakiya Douglas Nicholas P.J. Day Direk Limmathurotsakul Paul J. Brett Mary N. Burtnick |
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Vichaya Suttisunhakul |
title |
Evaluation of polysaccharide-based latex agglutination assays for the rapid detection of antibodies to burkholderia pseudomallei |
title_short |
Evaluation of polysaccharide-based latex agglutination assays for the rapid detection of antibodies to burkholderia pseudomallei |
title_full |
Evaluation of polysaccharide-based latex agglutination assays for the rapid detection of antibodies to burkholderia pseudomallei |
title_fullStr |
Evaluation of polysaccharide-based latex agglutination assays for the rapid detection of antibodies to burkholderia pseudomallei |
title_full_unstemmed |
Evaluation of polysaccharide-based latex agglutination assays for the rapid detection of antibodies to burkholderia pseudomallei |
title_sort |
evaluation of polysaccharide-based latex agglutination assays for the rapid detection of antibodies to burkholderia pseudomallei |
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2018 |
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https://repository.li.mahidol.ac.th/handle/123456789/36079 |
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1763496359859585024 |