Zinc cluster protein Znf1, a novel transcription factor of non-fermentative metabolism in Saccharomyces cerevisiae

© FEMS 2015. All rights reserved. For permissions, please e-mail: journals.permission@oup.com. The ability to rapidly respond to nutrient changes is a fundamental requirement for cell survival. Here, we show that the zinc cluster regulator Znf1 responds to altered nutrient signals following glucose...

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Main Authors: Pitchya Tangsombatvichit, Marta V. Semkiv, Andriy A. Sibirny, Laran T. Jensen, Khanok Ratanakhanokchai, Nitnipa Soontorngun
Other Authors: King Mongkuts University of Technology Thonburi
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Published: 2018
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Online Access:https://repository.li.mahidol.ac.th/handle/123456789/36122
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spelling th-mahidol.361222018-11-23T17:49:22Z Zinc cluster protein Znf1, a novel transcription factor of non-fermentative metabolism in Saccharomyces cerevisiae Pitchya Tangsombatvichit Marta V. Semkiv Andriy A. Sibirny Laran T. Jensen Khanok Ratanakhanokchai Nitnipa Soontorngun King Mongkuts University of Technology Thonburi Institute of Cell Biology National Academy of Sciences of Ukraine University of Rzeszow Mahidol University Immunology and Microbiology Medicine © FEMS 2015. All rights reserved. For permissions, please e-mail: journals.permission@oup.com. The ability to rapidly respond to nutrient changes is a fundamental requirement for cell survival. Here, we show that the zinc cluster regulator Znf1 responds to altered nutrient signals following glucose starvation through the direct control of genes involved in non-fermentative metabolism, including those belonged to the central pathways of gluconeogenesis (PCK1, FBP1 and MDH2), glyoxylate shunt (MLS1 and ICL1) and the tricarboxylic acid cycle (ACO1), which is demonstrated by Znf1-binding enrichment at these promoters during the glucose-ethanol shift. Additionally, reduced Pck1 and Fbp1 enzymatic activities correlate well with the data obtained from gene transcription analysis. Cells deleted for ZNF1 also display defective mitochondrial morphology with unclear structures of the inner membrane cristae when grown in ethanol, in agreement with the substantial reduction in the ATP content, suggesting for roles of Znf1 in maintaining mitochondrial morphology and function. Furthermore, Znf1 also plays a role in tolerance to pH and osmotic stress, especially during the oxidative metabolism. Taken together, our results clearly suggest that Znf1 is a critical transcriptional regulator for stress adaptation during non-fermentative growth with some partial overlapping targets with previously reported regulators in Saccharomyces cerevisiae. 2018-11-23T10:18:17Z 2018-11-23T10:18:17Z 2015-03-01 Article FEMS yeast research. Vol.15, No.2 (2015) 10.1093/femsyr/fou002 15671364 2-s2.0-85005808084 https://repository.li.mahidol.ac.th/handle/123456789/36122 Mahidol University SCOPUS https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85005808084&origin=inward
institution Mahidol University
building Mahidol University Library
continent Asia
country Thailand
Thailand
content_provider Mahidol University Library
collection Mahidol University Institutional Repository
topic Immunology and Microbiology
Medicine
spellingShingle Immunology and Microbiology
Medicine
Pitchya Tangsombatvichit
Marta V. Semkiv
Andriy A. Sibirny
Laran T. Jensen
Khanok Ratanakhanokchai
Nitnipa Soontorngun
Zinc cluster protein Znf1, a novel transcription factor of non-fermentative metabolism in Saccharomyces cerevisiae
description © FEMS 2015. All rights reserved. For permissions, please e-mail: journals.permission@oup.com. The ability to rapidly respond to nutrient changes is a fundamental requirement for cell survival. Here, we show that the zinc cluster regulator Znf1 responds to altered nutrient signals following glucose starvation through the direct control of genes involved in non-fermentative metabolism, including those belonged to the central pathways of gluconeogenesis (PCK1, FBP1 and MDH2), glyoxylate shunt (MLS1 and ICL1) and the tricarboxylic acid cycle (ACO1), which is demonstrated by Znf1-binding enrichment at these promoters during the glucose-ethanol shift. Additionally, reduced Pck1 and Fbp1 enzymatic activities correlate well with the data obtained from gene transcription analysis. Cells deleted for ZNF1 also display defective mitochondrial morphology with unclear structures of the inner membrane cristae when grown in ethanol, in agreement with the substantial reduction in the ATP content, suggesting for roles of Znf1 in maintaining mitochondrial morphology and function. Furthermore, Znf1 also plays a role in tolerance to pH and osmotic stress, especially during the oxidative metabolism. Taken together, our results clearly suggest that Znf1 is a critical transcriptional regulator for stress adaptation during non-fermentative growth with some partial overlapping targets with previously reported regulators in Saccharomyces cerevisiae.
author2 King Mongkuts University of Technology Thonburi
author_facet King Mongkuts University of Technology Thonburi
Pitchya Tangsombatvichit
Marta V. Semkiv
Andriy A. Sibirny
Laran T. Jensen
Khanok Ratanakhanokchai
Nitnipa Soontorngun
format Article
author Pitchya Tangsombatvichit
Marta V. Semkiv
Andriy A. Sibirny
Laran T. Jensen
Khanok Ratanakhanokchai
Nitnipa Soontorngun
author_sort Pitchya Tangsombatvichit
title Zinc cluster protein Znf1, a novel transcription factor of non-fermentative metabolism in Saccharomyces cerevisiae
title_short Zinc cluster protein Znf1, a novel transcription factor of non-fermentative metabolism in Saccharomyces cerevisiae
title_full Zinc cluster protein Znf1, a novel transcription factor of non-fermentative metabolism in Saccharomyces cerevisiae
title_fullStr Zinc cluster protein Znf1, a novel transcription factor of non-fermentative metabolism in Saccharomyces cerevisiae
title_full_unstemmed Zinc cluster protein Znf1, a novel transcription factor of non-fermentative metabolism in Saccharomyces cerevisiae
title_sort zinc cluster protein znf1, a novel transcription factor of non-fermentative metabolism in saccharomyces cerevisiae
publishDate 2018
url https://repository.li.mahidol.ac.th/handle/123456789/36122
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