Production of anti-CD3/28 expanded CD4<sup>+</sup> T lymphocytes from HIV-infected patients with different degrees of disease progression
© 2015 Future Medicine Ltd. Aims: CD4+ T lymphocytes from HIV-infected patients with different degrees of disease progression based on CD4 count were expanded in vitro using anti-CD3/28-coated beads. Materials & methods: Purified CD4+ T lymphocytes from healthy subjects and patients were expande...
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th-mahidol.361572018-11-23T17:20:48Z Production of anti-CD3/28 expanded CD4<sup>+</sup> T lymphocytes from HIV-infected patients with different degrees of disease progression Nattawat Onlamoon Vajee Petphong Kasama Sukapirom Siyu Wang Palanee Ammaranond Kovit Pattanapanyasat Mahidol University Chulalongkorn University Immunology and Microbiology © 2015 Future Medicine Ltd. Aims: CD4+ T lymphocytes from HIV-infected patients with different degrees of disease progression based on CD4 count were expanded in vitro using anti-CD3/28-coated beads. Materials & methods: Purified CD4+ T lymphocytes from healthy subjects and patients were expanded for 3 weeks. Moreover, the improvement of cell expansion by IL-2 supplementation was also determined. Results: Expanded CD4+ T lymphocytes from patients had lower fold expansion when compared with healthy subjects. Furthermore, patients with high CD4 counts had higher fold expansion than patients with low CD4 count, and IL-2 supplementation further increased cell expansion. Conclusions: Anti-CD3/28 activation failed to potently induce expansion of CD4+ T lymphocytes from patients. However, the cell expansion could be improved by IL-2 supplementation. 2018-11-23T10:20:48Z 2018-11-23T10:20:48Z 2015-01-01 Article Immunotherapy. Vol.7, No.7 (2015), 765-775 10.2217/imt.15.41 17507448 1750743X 2-s2.0-84942108152 https://repository.li.mahidol.ac.th/handle/123456789/36157 Mahidol University SCOPUS https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84942108152&origin=inward |
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Immunology and Microbiology Nattawat Onlamoon Vajee Petphong Kasama Sukapirom Siyu Wang Palanee Ammaranond Kovit Pattanapanyasat Production of anti-CD3/28 expanded CD4<sup>+</sup> T lymphocytes from HIV-infected patients with different degrees of disease progression |
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© 2015 Future Medicine Ltd. Aims: CD4+ T lymphocytes from HIV-infected patients with different degrees of disease progression based on CD4 count were expanded in vitro using anti-CD3/28-coated beads. Materials & methods: Purified CD4+ T lymphocytes from healthy subjects and patients were expanded for 3 weeks. Moreover, the improvement of cell expansion by IL-2 supplementation was also determined. Results: Expanded CD4+ T lymphocytes from patients had lower fold expansion when compared with healthy subjects. Furthermore, patients with high CD4 counts had higher fold expansion than patients with low CD4 count, and IL-2 supplementation further increased cell expansion. Conclusions: Anti-CD3/28 activation failed to potently induce expansion of CD4+ T lymphocytes from patients. However, the cell expansion could be improved by IL-2 supplementation. |
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Mahidol University |
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Mahidol University Nattawat Onlamoon Vajee Petphong Kasama Sukapirom Siyu Wang Palanee Ammaranond Kovit Pattanapanyasat |
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Article |
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Nattawat Onlamoon Vajee Petphong Kasama Sukapirom Siyu Wang Palanee Ammaranond Kovit Pattanapanyasat |
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Nattawat Onlamoon |
title |
Production of anti-CD3/28 expanded CD4<sup>+</sup> T lymphocytes from HIV-infected patients with different degrees of disease progression |
title_short |
Production of anti-CD3/28 expanded CD4<sup>+</sup> T lymphocytes from HIV-infected patients with different degrees of disease progression |
title_full |
Production of anti-CD3/28 expanded CD4<sup>+</sup> T lymphocytes from HIV-infected patients with different degrees of disease progression |
title_fullStr |
Production of anti-CD3/28 expanded CD4<sup>+</sup> T lymphocytes from HIV-infected patients with different degrees of disease progression |
title_full_unstemmed |
Production of anti-CD3/28 expanded CD4<sup>+</sup> T lymphocytes from HIV-infected patients with different degrees of disease progression |
title_sort |
production of anti-cd3/28 expanded cd4<sup>+</sup> t lymphocytes from hiv-infected patients with different degrees of disease progression |
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2018 |
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https://repository.li.mahidol.ac.th/handle/123456789/36157 |
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1763494225584848896 |