Validation of the immunochromatographic strip for α-thalassemia screening: A multicenter study
© 2015 Elsevier Inc. All rights reserved. α0-Thalassemia occurs from a deletion of 2 linked α-globin genes and interaction of these defective genes leads to hemoglobin (Hb) Bart's hydrops fetalis, the most severe and lethal thalassemia syndrome. Identification of α0-thalassemia carriers is thus...
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th-mahidol.364172018-11-23T17:43:14Z Validation of the immunochromatographic strip for α-thalassemia screening: A multicenter study Pranee Winichagoon Pornnapa Kumpan Paula Holmes Jill Finlayson Christopher Newbound Arnold Kabral Benjamin Li Manit Nuinoon Terry Fawcett Chatchai Tayapiwatana Watchara Kasinrerk Suthat Fucharoen Mahidol University University of Western Australia Medicine © 2015 Elsevier Inc. All rights reserved. α0-Thalassemia occurs from a deletion of 2 linked α-globin genes and interaction of these defective genes leads to hemoglobin (Hb) Bart's hydrops fetalis, the most severe and lethal thalassemia syndrome. Identification of α0-thalassemia carriers is thus essential for the prevention and control program. An immunochromatographic (IC) strip test was developed for rapid screening of α0-thalassemia by testing for Hb Bart's in the blood samples using a specific monoclonal antibody against Hb Bart's. To evaluate its sensitivity and specificity, the IC strip test was assessed in a cohort with various thalassemia genotypes from 4 different laboratories in Thailand and Australia. The result showed 97% sensitivity in α-thalassemia carriers with 2 α-globin genes deletion and Hb H disease. This is, in particular, the useful rapid screening test for regions where β-thalassemia and homozygous Hb E are also common. Similar hematologic and Hb data make it impossible to address the concomitant inheritance of α0-thalassemia in these samples without polymerase chain reaction (PCR)-based techniques, leading to misdiagnosis of the risk of having Hb Bart's hydrops fetalis. However, α-globin genotyping should be carried out in samples with positive IC strip as positive reactivity was also observed in homozygous α+-thalassemia carriers who have 2 trans α-globin gene deletions. These results indicate that in combination with red blood cell indices, the IC strip test could rule out mass populations for further α0-thalassemia detection by PCR-based analysis. The Alpha Thal IC strip also has the potential to replace testing for Hb H inclusion bodies, as it appears to be more sensitive, specific, and less labor intensive. 2018-11-23T10:43:14Z 2018-11-23T10:43:14Z 2015-06-01 Article Translational Research. Vol.165, No.6 (2015), 689-695 10.1016/j.trsl.2014.10.013 18781810 19315244 2-s2.0-84929191929 https://repository.li.mahidol.ac.th/handle/123456789/36417 Mahidol University SCOPUS https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84929191929&origin=inward |
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Medicine Pranee Winichagoon Pornnapa Kumpan Paula Holmes Jill Finlayson Christopher Newbound Arnold Kabral Benjamin Li Manit Nuinoon Terry Fawcett Chatchai Tayapiwatana Watchara Kasinrerk Suthat Fucharoen Validation of the immunochromatographic strip for α-thalassemia screening: A multicenter study |
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© 2015 Elsevier Inc. All rights reserved. α0-Thalassemia occurs from a deletion of 2 linked α-globin genes and interaction of these defective genes leads to hemoglobin (Hb) Bart's hydrops fetalis, the most severe and lethal thalassemia syndrome. Identification of α0-thalassemia carriers is thus essential for the prevention and control program. An immunochromatographic (IC) strip test was developed for rapid screening of α0-thalassemia by testing for Hb Bart's in the blood samples using a specific monoclonal antibody against Hb Bart's. To evaluate its sensitivity and specificity, the IC strip test was assessed in a cohort with various thalassemia genotypes from 4 different laboratories in Thailand and Australia. The result showed 97% sensitivity in α-thalassemia carriers with 2 α-globin genes deletion and Hb H disease. This is, in particular, the useful rapid screening test for regions where β-thalassemia and homozygous Hb E are also common. Similar hematologic and Hb data make it impossible to address the concomitant inheritance of α0-thalassemia in these samples without polymerase chain reaction (PCR)-based techniques, leading to misdiagnosis of the risk of having Hb Bart's hydrops fetalis. However, α-globin genotyping should be carried out in samples with positive IC strip as positive reactivity was also observed in homozygous α+-thalassemia carriers who have 2 trans α-globin gene deletions. These results indicate that in combination with red blood cell indices, the IC strip test could rule out mass populations for further α0-thalassemia detection by PCR-based analysis. The Alpha Thal IC strip also has the potential to replace testing for Hb H inclusion bodies, as it appears to be more sensitive, specific, and less labor intensive. |
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Mahidol University |
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Mahidol University Pranee Winichagoon Pornnapa Kumpan Paula Holmes Jill Finlayson Christopher Newbound Arnold Kabral Benjamin Li Manit Nuinoon Terry Fawcett Chatchai Tayapiwatana Watchara Kasinrerk Suthat Fucharoen |
format |
Article |
author |
Pranee Winichagoon Pornnapa Kumpan Paula Holmes Jill Finlayson Christopher Newbound Arnold Kabral Benjamin Li Manit Nuinoon Terry Fawcett Chatchai Tayapiwatana Watchara Kasinrerk Suthat Fucharoen |
author_sort |
Pranee Winichagoon |
title |
Validation of the immunochromatographic strip for α-thalassemia screening: A multicenter study |
title_short |
Validation of the immunochromatographic strip for α-thalassemia screening: A multicenter study |
title_full |
Validation of the immunochromatographic strip for α-thalassemia screening: A multicenter study |
title_fullStr |
Validation of the immunochromatographic strip for α-thalassemia screening: A multicenter study |
title_full_unstemmed |
Validation of the immunochromatographic strip for α-thalassemia screening: A multicenter study |
title_sort |
validation of the immunochromatographic strip for α-thalassemia screening: a multicenter study |
publishDate |
2018 |
url |
https://repository.li.mahidol.ac.th/handle/123456789/36417 |
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1763496712066826240 |