Cellufine sulfate column chromatography as a simple, rapid, and effective method to purify dengue virus

Cellufine sulfate column chromatography as a simple, rapid, and effective method to purify dengue virus

© 2016 Elsevier B.V. Conventional method to purify/concentrate dengue virus (DENV) is time-consuming with low virus recovery yield. Herein, we applied cellufine sulfate column chromatography to purify/concentrate DENV based on the mimicry between heparan sulfate and DENV envelope protein. Comparativ...

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Main Authors: Rattiyaporn Kanlaya, Visith Thongboonkerd
Other Authors: Mahidol University
Format: Article
Published: 2018
Subjects:
Immunology and Microbiology
Online Access:https://repository.li.mahidol.ac.th/handle/123456789/40782
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spelling th-mahidol.407822019-03-14T15:01:42Z Cellufine sulfate column chromatography as a simple, rapid, and effective method to purify dengue virus Rattiyaporn Kanlaya Visith Thongboonkerd Mahidol University Immunology and Microbiology © 2016 Elsevier B.V. Conventional method to purify/concentrate dengue virus (DENV) is time-consuming with low virus recovery yield. Herein, we applied cellufine sulfate column chromatography to purify/concentrate DENV based on the mimicry between heparan sulfate and DENV envelope protein. Comparative analysis demonstrated that this new method offered higher purity (as determined by less contamination of bovine serum albumin) and recovery yield (as determined by greater infectivity). Moreover, overall duration used for cellufine sulfate column chromatography to purify/concentrate DENV was approximately 1/20 of that of conventional method. Therefore, cellufine sulfate column chromatography serves as a simple, rapid, and effective alternative method for DENV purification/concentration. 2018-12-11T02:59:42Z 2019-03-14T08:01:42Z 2018-12-11T02:59:42Z 2019-03-14T08:01:42Z 2016-08-01 Article Journal of Virological Methods. Vol.234, (2016), 174-177 10.1016/j.jviromet.2016.04.023 18790984 01660934 2-s2.0-84965052635 https://repository.li.mahidol.ac.th/handle/123456789/40782 Mahidol University SCOPUS https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84965052635&origin=inward
institution Mahidol University
building Mahidol University Library
continent Asia
country Thailand
Thailand
content_provider Mahidol University Library
collection Mahidol University Institutional Repository
topic Immunology and Microbiology
spellingShingle Immunology and Microbiology
Rattiyaporn Kanlaya
Visith Thongboonkerd
Cellufine sulfate column chromatography as a simple, rapid, and effective method to purify dengue virus
description © 2016 Elsevier B.V. Conventional method to purify/concentrate dengue virus (DENV) is time-consuming with low virus recovery yield. Herein, we applied cellufine sulfate column chromatography to purify/concentrate DENV based on the mimicry between heparan sulfate and DENV envelope protein. Comparative analysis demonstrated that this new method offered higher purity (as determined by less contamination of bovine serum albumin) and recovery yield (as determined by greater infectivity). Moreover, overall duration used for cellufine sulfate column chromatography to purify/concentrate DENV was approximately 1/20 of that of conventional method. Therefore, cellufine sulfate column chromatography serves as a simple, rapid, and effective alternative method for DENV purification/concentration.
author2 Mahidol University
author_facet Mahidol University
Rattiyaporn Kanlaya
Visith Thongboonkerd
format Article
author Rattiyaporn Kanlaya
Visith Thongboonkerd
author_sort Rattiyaporn Kanlaya
title Cellufine sulfate column chromatography as a simple, rapid, and effective method to purify dengue virus
title_short Cellufine sulfate column chromatography as a simple, rapid, and effective method to purify dengue virus
title_full Cellufine sulfate column chromatography as a simple, rapid, and effective method to purify dengue virus
title_fullStr Cellufine sulfate column chromatography as a simple, rapid, and effective method to purify dengue virus
title_full_unstemmed Cellufine sulfate column chromatography as a simple, rapid, and effective method to purify dengue virus
title_sort cellufine sulfate column chromatography as a simple, rapid, and effective method to purify dengue virus
publishDate 2018
url https://repository.li.mahidol.ac.th/handle/123456789/40782
_version_ 1763495505725227008

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