Comparison of a new in-house and three published HLA-B∗15:02 screening methods for prevention of carbamazepine-induced severe drug reactions
© 2016 Jaruthamsophon et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Currently, there are three publishe...
Saved in:
Main Authors: | , , , |
---|---|
Other Authors: | |
Format: | Article |
Published: |
2018
|
Subjects: | |
Online Access: | https://repository.li.mahidol.ac.th/handle/123456789/40804 |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
Institution: | Mahidol University |
id |
th-mahidol.40804 |
---|---|
record_format |
dspace |
spelling |
th-mahidol.408042019-03-14T15:01:43Z Comparison of a new in-house and three published HLA-B∗15:02 screening methods for prevention of carbamazepine-induced severe drug reactions Kanoot Jaruthamsophon Thanya Sripo Chonlaphat Sukasem Pornprot Limprasert Prince of Songkla University Mahidol University Agricultural and Biological Sciences Biochemistry, Genetics and Molecular Biology © 2016 Jaruthamsophon et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Currently, there are three published HLA-B∗15:02 screening methods for prevention of carbamazepine-induced severe drug reactions in Asian populations. To analyze available HLA-B∗15:02 screening methods, we compared four screening methods, including a multiplex PCR method, a nested PCR method, a LAMP method and our new in-house PCR-dot blot hybridization method. These methods were reviewed regarding their sensitivity, specificity, false positivity and technical considerations. Possible false positive (FP) alleles and genotypes were checked regarding the primers and probes designs, using the IMGT/HLA database. Expected FP rates in Asian populations were predicted using the Allele Frequencies Net Database. All methods had a sensitivity of more than 99.9%, although giving FP results to certain very rare alleles and genotypes. The multiplex PCR method was the only test that gave FP results to certain genotypes of HLA-B∗15:13, the allele which is prevalent in Southeast Asian populations. In conclusion, the nested PCR, LAMP and our in-house methods could be applied in various Asian populations, but the multiplex PCR, or any test with FP to HLA-B∗15:13, should be applied with caution in the Southeast Asian populations. 2018-12-11T01:59:54Z 2019-03-14T08:01:43Z 2018-12-11T01:59:54Z 2019-03-14T08:01:43Z 2016-05-01 Article PLoS ONE. Vol.11, No.5 (2016) 10.1371/journal.pone.0155907 19326203 2-s2.0-84973632360 https://repository.li.mahidol.ac.th/handle/123456789/40804 Mahidol University SCOPUS https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84973632360&origin=inward |
institution |
Mahidol University |
building |
Mahidol University Library |
continent |
Asia |
country |
Thailand Thailand |
content_provider |
Mahidol University Library |
collection |
Mahidol University Institutional Repository |
topic |
Agricultural and Biological Sciences Biochemistry, Genetics and Molecular Biology |
spellingShingle |
Agricultural and Biological Sciences Biochemistry, Genetics and Molecular Biology Kanoot Jaruthamsophon Thanya Sripo Chonlaphat Sukasem Pornprot Limprasert Comparison of a new in-house and three published HLA-B∗15:02 screening methods for prevention of carbamazepine-induced severe drug reactions |
description |
© 2016 Jaruthamsophon et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Currently, there are three published HLA-B∗15:02 screening methods for prevention of carbamazepine-induced severe drug reactions in Asian populations. To analyze available HLA-B∗15:02 screening methods, we compared four screening methods, including a multiplex PCR method, a nested PCR method, a LAMP method and our new in-house PCR-dot blot hybridization method. These methods were reviewed regarding their sensitivity, specificity, false positivity and technical considerations. Possible false positive (FP) alleles and genotypes were checked regarding the primers and probes designs, using the IMGT/HLA database. Expected FP rates in Asian populations were predicted using the Allele Frequencies Net Database. All methods had a sensitivity of more than 99.9%, although giving FP results to certain very rare alleles and genotypes. The multiplex PCR method was the only test that gave FP results to certain genotypes of HLA-B∗15:13, the allele which is prevalent in Southeast Asian populations. In conclusion, the nested PCR, LAMP and our in-house methods could be applied in various Asian populations, but the multiplex PCR, or any test with FP to HLA-B∗15:13, should be applied with caution in the Southeast Asian populations. |
author2 |
Prince of Songkla University |
author_facet |
Prince of Songkla University Kanoot Jaruthamsophon Thanya Sripo Chonlaphat Sukasem Pornprot Limprasert |
format |
Article |
author |
Kanoot Jaruthamsophon Thanya Sripo Chonlaphat Sukasem Pornprot Limprasert |
author_sort |
Kanoot Jaruthamsophon |
title |
Comparison of a new in-house and three published HLA-B∗15:02 screening methods for prevention of carbamazepine-induced severe drug reactions |
title_short |
Comparison of a new in-house and three published HLA-B∗15:02 screening methods for prevention of carbamazepine-induced severe drug reactions |
title_full |
Comparison of a new in-house and three published HLA-B∗15:02 screening methods for prevention of carbamazepine-induced severe drug reactions |
title_fullStr |
Comparison of a new in-house and three published HLA-B∗15:02 screening methods for prevention of carbamazepine-induced severe drug reactions |
title_full_unstemmed |
Comparison of a new in-house and three published HLA-B∗15:02 screening methods for prevention of carbamazepine-induced severe drug reactions |
title_sort |
comparison of a new in-house and three published hla-b∗15:02 screening methods for prevention of carbamazepine-induced severe drug reactions |
publishDate |
2018 |
url |
https://repository.li.mahidol.ac.th/handle/123456789/40804 |
_version_ |
1763496314117554176 |