Gene models, expression repertoire, and immune response of Plasmodium vivax reticulocyte binding proteins
© 2016 Hietanen et al.. Members of the Plasmodium vivax reticulocyte binding protein (PvRBP) family are believed to mediate specific invasion of reticulocytes by P. vivax. In this study, we performed molecular characterization of genes encoding members of this protein family. Through cDNA sequencing...
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th-mahidol.408192019-03-14T15:01:44Z Gene models, expression repertoire, and immune response of Plasmodium vivax reticulocyte binding proteins Jenni Hietanen Anongruk Chim-Ong Thanprakorn Chiramanewong Jakub Gruszczyk Wanlapa Roobsoong Wai Hong Tham Jetsumon Sattabongkot Wang Nguitragool Mahidol University Walter and Eliza Hall Institute of Medical Research University of Melbourne Immunology and Microbiology © 2016 Hietanen et al.. Members of the Plasmodium vivax reticulocyte binding protein (PvRBP) family are believed to mediate specific invasion of reticulocytes by P. vivax. In this study, we performed molecular characterization of genes encoding members of this protein family. Through cDNA sequencing, we constructed full-length gene models and verified genes that are protein coding and those that are pseudogenes. We also used quantitative PCR to measure their in vivo transcript abundances in clinical P. vivax isolates. Like genes encoding related invasion ligands of P. falciparum, Pvrbp expression levels vary broadly across different parasite isolates. Through antibody measurements, we found that host immune pressure may be the driving force behind the distinctly high diversity of one of the family members, PvRBP2c. Mild yet significant negative correlation was found between parasitemia and the PvRBP2b antibody level, suggesting that antibodies to the protein may interfere with invasion. 2018-12-11T03:04:36Z 2019-03-14T08:01:44Z 2018-12-11T03:04:36Z 2019-03-14T08:01:44Z 2016-03-01 Article Infection and Immunity. Vol.84, No.3 (2016), 677-685 10.1128/IAI.01117-15 10985522 00199567 2-s2.0-84962198877 https://repository.li.mahidol.ac.th/handle/123456789/40819 Mahidol University SCOPUS https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84962198877&origin=inward |
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Immunology and Microbiology Jenni Hietanen Anongruk Chim-Ong Thanprakorn Chiramanewong Jakub Gruszczyk Wanlapa Roobsoong Wai Hong Tham Jetsumon Sattabongkot Wang Nguitragool Gene models, expression repertoire, and immune response of Plasmodium vivax reticulocyte binding proteins |
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© 2016 Hietanen et al.. Members of the Plasmodium vivax reticulocyte binding protein (PvRBP) family are believed to mediate specific invasion of reticulocytes by P. vivax. In this study, we performed molecular characterization of genes encoding members of this protein family. Through cDNA sequencing, we constructed full-length gene models and verified genes that are protein coding and those that are pseudogenes. We also used quantitative PCR to measure their in vivo transcript abundances in clinical P. vivax isolates. Like genes encoding related invasion ligands of P. falciparum, Pvrbp expression levels vary broadly across different parasite isolates. Through antibody measurements, we found that host immune pressure may be the driving force behind the distinctly high diversity of one of the family members, PvRBP2c. Mild yet significant negative correlation was found between parasitemia and the PvRBP2b antibody level, suggesting that antibodies to the protein may interfere with invasion. |
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Mahidol University |
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Mahidol University Jenni Hietanen Anongruk Chim-Ong Thanprakorn Chiramanewong Jakub Gruszczyk Wanlapa Roobsoong Wai Hong Tham Jetsumon Sattabongkot Wang Nguitragool |
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Article |
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Jenni Hietanen Anongruk Chim-Ong Thanprakorn Chiramanewong Jakub Gruszczyk Wanlapa Roobsoong Wai Hong Tham Jetsumon Sattabongkot Wang Nguitragool |
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Jenni Hietanen |
title |
Gene models, expression repertoire, and immune response of Plasmodium vivax reticulocyte binding proteins |
title_short |
Gene models, expression repertoire, and immune response of Plasmodium vivax reticulocyte binding proteins |
title_full |
Gene models, expression repertoire, and immune response of Plasmodium vivax reticulocyte binding proteins |
title_fullStr |
Gene models, expression repertoire, and immune response of Plasmodium vivax reticulocyte binding proteins |
title_full_unstemmed |
Gene models, expression repertoire, and immune response of Plasmodium vivax reticulocyte binding proteins |
title_sort |
gene models, expression repertoire, and immune response of plasmodium vivax reticulocyte binding proteins |
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2018 |
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https://repository.li.mahidol.ac.th/handle/123456789/40819 |
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1763490920694546432 |