Excretory-secretory product of third-stage Gnathostoma spinigerum larvae induces apoptosis in human peripheral blood mononuclear cells

© 2017, Springer-Verlag GmbH Germany. Human gnathostomiasis caused by third-stage Gnathostoma spinigerum larvae (G. spinigerum L3) is an important zoonotic disease in tropical areas of the world. The excretory-secretory products (ES) that are excreted by infective larva play a significant role in ho...

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Main Authors: Nareerat Viseshakul, Wilanee Dechkhajorn, Surachet Benjathummarak, Supaporn Nuamtanong, Yaowapa Maneerat
Other Authors: Chulalongkorn University
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Published: 2018
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Online Access:https://repository.li.mahidol.ac.th/handle/123456789/41328
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spelling th-mahidol.413282019-03-14T15:02:16Z Excretory-secretory product of third-stage Gnathostoma spinigerum larvae induces apoptosis in human peripheral blood mononuclear cells Nareerat Viseshakul Wilanee Dechkhajorn Surachet Benjathummarak Supaporn Nuamtanong Yaowapa Maneerat Chulalongkorn University Mahidol University Agricultural and Biological Sciences Immunology and Microbiology © 2017, Springer-Verlag GmbH Germany. Human gnathostomiasis caused by third-stage Gnathostoma spinigerum larvae (G. spinigerum L3) is an important zoonotic disease in tropical areas of the world. The excretory-secretory products (ES) that are excreted by infective larva play a significant role in host immune evasion and tissue destruction. To investigate the poorly understood mechanisms of G. spinigerum L3 pathogenesis, we focused on the potential effect of ES on inducing apoptosis in human immune cells by using human peripheral blood mononuclear cells (PBMCs) as a model. Early and late apoptosis of PBMCs were assessed following the exposure of these cells to G. spinigerum L3 ES (0.1, 0.5, and 1.0 μg/ml) for 6–48 h. The apoptotic cells were identified by flow cytometric staining of PBMC with FITC-annexin V and propidium iodide. The expression of regulatory genes related to apoptosis mechanisms in ES-treated PBMCs was investigated using a Human Apoptosis RT2 Profiler™ PCR Array. The results showed significant levels of early phase apoptosis at 18 h and of late phase apoptosis at 24 h. We speculate that this apoptosis in PBMCs occurs via the extrinsic pathway. Apoptosis in the ES-induced PBMCs was observed as quickly as 90 min after exposure, and the highest effect was observed at 18–24 h. Furthermore, ES can trigger apoptosis lasting for 48 h. Our findings expand the understanding of one of the mechanisms involved, immune-evasive strategy mechanism used by G. spinigerum larvae during human gnathostomiasis. 2018-12-21T06:25:05Z 2019-03-14T08:02:16Z 2018-12-21T06:25:05Z 2019-03-14T08:02:16Z 2017-10-01 Article Parasitology Research. Vol.116, No.10 (2017), 2783-2794 10.1007/s00436-017-5589-5 14321955 09320113 2-s2.0-85028023806 https://repository.li.mahidol.ac.th/handle/123456789/41328 Mahidol University SCOPUS https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85028023806&origin=inward
institution Mahidol University
building Mahidol University Library
continent Asia
country Thailand
Thailand
content_provider Mahidol University Library
collection Mahidol University Institutional Repository
topic Agricultural and Biological Sciences
Immunology and Microbiology
spellingShingle Agricultural and Biological Sciences
Immunology and Microbiology
Nareerat Viseshakul
Wilanee Dechkhajorn
Surachet Benjathummarak
Supaporn Nuamtanong
Yaowapa Maneerat
Excretory-secretory product of third-stage Gnathostoma spinigerum larvae induces apoptosis in human peripheral blood mononuclear cells
description © 2017, Springer-Verlag GmbH Germany. Human gnathostomiasis caused by third-stage Gnathostoma spinigerum larvae (G. spinigerum L3) is an important zoonotic disease in tropical areas of the world. The excretory-secretory products (ES) that are excreted by infective larva play a significant role in host immune evasion and tissue destruction. To investigate the poorly understood mechanisms of G. spinigerum L3 pathogenesis, we focused on the potential effect of ES on inducing apoptosis in human immune cells by using human peripheral blood mononuclear cells (PBMCs) as a model. Early and late apoptosis of PBMCs were assessed following the exposure of these cells to G. spinigerum L3 ES (0.1, 0.5, and 1.0 μg/ml) for 6–48 h. The apoptotic cells were identified by flow cytometric staining of PBMC with FITC-annexin V and propidium iodide. The expression of regulatory genes related to apoptosis mechanisms in ES-treated PBMCs was investigated using a Human Apoptosis RT2 Profiler™ PCR Array. The results showed significant levels of early phase apoptosis at 18 h and of late phase apoptosis at 24 h. We speculate that this apoptosis in PBMCs occurs via the extrinsic pathway. Apoptosis in the ES-induced PBMCs was observed as quickly as 90 min after exposure, and the highest effect was observed at 18–24 h. Furthermore, ES can trigger apoptosis lasting for 48 h. Our findings expand the understanding of one of the mechanisms involved, immune-evasive strategy mechanism used by G. spinigerum larvae during human gnathostomiasis.
author2 Chulalongkorn University
author_facet Chulalongkorn University
Nareerat Viseshakul
Wilanee Dechkhajorn
Surachet Benjathummarak
Supaporn Nuamtanong
Yaowapa Maneerat
format Article
author Nareerat Viseshakul
Wilanee Dechkhajorn
Surachet Benjathummarak
Supaporn Nuamtanong
Yaowapa Maneerat
author_sort Nareerat Viseshakul
title Excretory-secretory product of third-stage Gnathostoma spinigerum larvae induces apoptosis in human peripheral blood mononuclear cells
title_short Excretory-secretory product of third-stage Gnathostoma spinigerum larvae induces apoptosis in human peripheral blood mononuclear cells
title_full Excretory-secretory product of third-stage Gnathostoma spinigerum larvae induces apoptosis in human peripheral blood mononuclear cells
title_fullStr Excretory-secretory product of third-stage Gnathostoma spinigerum larvae induces apoptosis in human peripheral blood mononuclear cells
title_full_unstemmed Excretory-secretory product of third-stage Gnathostoma spinigerum larvae induces apoptosis in human peripheral blood mononuclear cells
title_sort excretory-secretory product of third-stage gnathostoma spinigerum larvae induces apoptosis in human peripheral blood mononuclear cells
publishDate 2018
url https://repository.li.mahidol.ac.th/handle/123456789/41328
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