Evidence of TiLV infection in tilapia hatcheries in Thailand from 2012 to 2017 reveals probable global spread of the disease
© 2017 Elsevier B.V. Recent outbreaks of tilapia lake virus (TiLV) in farmed tilapia in Thailand were the first indication of spread of the virus to the Southeast Asia region. Here we further investigate TiLV infection of archived and newly collected fish samples obtained from Thai hatcheries from 2...
Saved in:
Main Authors: | , , , , |
---|---|
Other Authors: | |
Format: | Article |
Published: |
2018
|
Subjects: | |
Online Access: | https://repository.li.mahidol.ac.th/handle/123456789/41352 |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
Institution: | Mahidol University |
id |
th-mahidol.41352 |
---|---|
record_format |
dspace |
spelling |
th-mahidol.413522019-03-14T15:02:18Z Evidence of TiLV infection in tilapia hatcheries in Thailand from 2012 to 2017 reveals probable global spread of the disease H. T. Dong G. A. Ataguba P. Khunrae T. Rattanarojpong S. Senapin King Mongkut s University of Technology Thonburi Mahidol University University of Agriculture, Makurdi Thailand National Science and Technology Development Agency Agricultural and Biological Sciences © 2017 Elsevier B.V. Recent outbreaks of tilapia lake virus (TiLV) in farmed tilapia in Thailand were the first indication of spread of the virus to the Southeast Asia region. Here we further investigate TiLV infection of archived and newly collected fish samples obtained from Thai hatcheries from 2012 to 2017. Fertilized eggs, yolk-sac larvae, fries, and fingerlings were tested for the TiLV using an established semi-nested RT-PCR assay. The results revealed that the majority of the tested samples were TiLV positive, including our earliest preserved samples collected in year 2012. DNA sequence analysis of representative amplified products also confirmed the presence of TiLV. Since the discovery of TiLV in 2012, over 40 countries worldwide have imported tilapia fry and fingerlings, and some may have been unaware of risk that they might be infected with TiLV. Thus, if they have not already done so, we recommend that countries that have imported tilapia for aquaculture carry out surveillance studies for its presence and also add TiLV to their import quarantine inspection list. 2018-12-21T06:25:04Z 2019-03-14T08:02:18Z 2018-12-21T06:25:04Z 2019-03-14T08:02:18Z 2017-10-01 Article Aquaculture. Vol.479, (2017), 579-583 10.1016/j.aquaculture.2017.06.035 00448486 2-s2.0-85021426472 https://repository.li.mahidol.ac.th/handle/123456789/41352 Mahidol University SCOPUS https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85021426472&origin=inward |
institution |
Mahidol University |
building |
Mahidol University Library |
continent |
Asia |
country |
Thailand Thailand |
content_provider |
Mahidol University Library |
collection |
Mahidol University Institutional Repository |
topic |
Agricultural and Biological Sciences |
spellingShingle |
Agricultural and Biological Sciences H. T. Dong G. A. Ataguba P. Khunrae T. Rattanarojpong S. Senapin Evidence of TiLV infection in tilapia hatcheries in Thailand from 2012 to 2017 reveals probable global spread of the disease |
description |
© 2017 Elsevier B.V. Recent outbreaks of tilapia lake virus (TiLV) in farmed tilapia in Thailand were the first indication of spread of the virus to the Southeast Asia region. Here we further investigate TiLV infection of archived and newly collected fish samples obtained from Thai hatcheries from 2012 to 2017. Fertilized eggs, yolk-sac larvae, fries, and fingerlings were tested for the TiLV using an established semi-nested RT-PCR assay. The results revealed that the majority of the tested samples were TiLV positive, including our earliest preserved samples collected in year 2012. DNA sequence analysis of representative amplified products also confirmed the presence of TiLV. Since the discovery of TiLV in 2012, over 40 countries worldwide have imported tilapia fry and fingerlings, and some may have been unaware of risk that they might be infected with TiLV. Thus, if they have not already done so, we recommend that countries that have imported tilapia for aquaculture carry out surveillance studies for its presence and also add TiLV to their import quarantine inspection list. |
author2 |
King Mongkut s University of Technology Thonburi |
author_facet |
King Mongkut s University of Technology Thonburi H. T. Dong G. A. Ataguba P. Khunrae T. Rattanarojpong S. Senapin |
format |
Article |
author |
H. T. Dong G. A. Ataguba P. Khunrae T. Rattanarojpong S. Senapin |
author_sort |
H. T. Dong |
title |
Evidence of TiLV infection in tilapia hatcheries in Thailand from 2012 to 2017 reveals probable global spread of the disease |
title_short |
Evidence of TiLV infection in tilapia hatcheries in Thailand from 2012 to 2017 reveals probable global spread of the disease |
title_full |
Evidence of TiLV infection in tilapia hatcheries in Thailand from 2012 to 2017 reveals probable global spread of the disease |
title_fullStr |
Evidence of TiLV infection in tilapia hatcheries in Thailand from 2012 to 2017 reveals probable global spread of the disease |
title_full_unstemmed |
Evidence of TiLV infection in tilapia hatcheries in Thailand from 2012 to 2017 reveals probable global spread of the disease |
title_sort |
evidence of tilv infection in tilapia hatcheries in thailand from 2012 to 2017 reveals probable global spread of the disease |
publishDate |
2018 |
url |
https://repository.li.mahidol.ac.th/handle/123456789/41352 |
_version_ |
1763491568590782464 |