Genetic differences among Haplorchis taichui populations in Indochina revealed by mitochondrial COX1 sequences

Copyright © Cambridge University Press 2016. Haplorchis taichui is an intestinal heterophyid fluke that is pathogenic to humans. It is widely distributed in Asia, with a particularly high prevalence in Indochina. Previous work revealed that the lack of gene flow between three distinct populations of...

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Main Authors: U. Thaenkham, O. Phuphisut, S. Nuamtanong, T. Yoonuan, S. Sa-Nguankiat, Y. Vonghachack, V. Y. Belizario, D. T. Dung, P. Dekumyoy, J. Waikagul
Other Authors: Mahidol University
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Published: 2018
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Online Access:https://repository.li.mahidol.ac.th/handle/123456789/41391
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spelling th-mahidol.413912019-03-14T15:02:21Z Genetic differences among Haplorchis taichui populations in Indochina revealed by mitochondrial COX1 sequences U. Thaenkham O. Phuphisut S. Nuamtanong T. Yoonuan S. Sa-Nguankiat Y. Vonghachack V. Y. Belizario D. T. Dung P. Dekumyoy J. Waikagul Mahidol University University of Health Sciences University of the Philippines Manila National Institute of Malariology and Entomology Agricultural and Biological Sciences Immunology and Microbiology Copyright © Cambridge University Press 2016. Haplorchis taichui is an intestinal heterophyid fluke that is pathogenic to humans. It is widely distributed in Asia, with a particularly high prevalence in Indochina. Previous work revealed that the lack of gene flow between three distinct populations of Vietnamese H. taichui can be attributed to their geographic isolation with no interconnected river basins. To test the hypothesis that interconnected river basins allow gene flow between otherwise isolated populations of H. taichui, as previously demonstrated for another trematode, Opisthorchis viverrini, we compared the genetic structures of seven populations of H. taichui from various localities in the lower Mekong Basin, in Thailand and Laos, with those in Vietnam, using the mitochondrial cytochrome c oxidase subunit 1 (COX1) gene. To determine the gene flow between these H. taichui populations, we calculated their phylogenetic relationships, genetic distances and haplotype diversity. Each population showed very low nucleotide diversity at this locus. However, high levels of genetic differentiation between the populations indicated very little gene flow. A phylogenetic analysis divided the populations into four clusters that correlated with the country of origin. The negligible gene flow between the Thai and Laos populations, despite sharing the Mekong Basin, caused us to reject our hypothesis. Our data suggest that the distribution of H. taichui populations was incidentally associated with national borders. 2018-12-21T06:26:00Z 2019-03-14T08:02:21Z 2018-12-21T06:26:00Z 2019-03-14T08:02:21Z 2017-09-01 Article Journal of Helminthology. Vol.91, No.5 (2017), 597-604 10.1017/S0022149X1600050X 14752697 0022149X 2-s2.0-84978539103 https://repository.li.mahidol.ac.th/handle/123456789/41391 Mahidol University SCOPUS https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84978539103&origin=inward
institution Mahidol University
building Mahidol University Library
continent Asia
country Thailand
Thailand
content_provider Mahidol University Library
collection Mahidol University Institutional Repository
topic Agricultural and Biological Sciences
Immunology and Microbiology
spellingShingle Agricultural and Biological Sciences
Immunology and Microbiology
U. Thaenkham
O. Phuphisut
S. Nuamtanong
T. Yoonuan
S. Sa-Nguankiat
Y. Vonghachack
V. Y. Belizario
D. T. Dung
P. Dekumyoy
J. Waikagul
Genetic differences among Haplorchis taichui populations in Indochina revealed by mitochondrial COX1 sequences
description Copyright © Cambridge University Press 2016. Haplorchis taichui is an intestinal heterophyid fluke that is pathogenic to humans. It is widely distributed in Asia, with a particularly high prevalence in Indochina. Previous work revealed that the lack of gene flow between three distinct populations of Vietnamese H. taichui can be attributed to their geographic isolation with no interconnected river basins. To test the hypothesis that interconnected river basins allow gene flow between otherwise isolated populations of H. taichui, as previously demonstrated for another trematode, Opisthorchis viverrini, we compared the genetic structures of seven populations of H. taichui from various localities in the lower Mekong Basin, in Thailand and Laos, with those in Vietnam, using the mitochondrial cytochrome c oxidase subunit 1 (COX1) gene. To determine the gene flow between these H. taichui populations, we calculated their phylogenetic relationships, genetic distances and haplotype diversity. Each population showed very low nucleotide diversity at this locus. However, high levels of genetic differentiation between the populations indicated very little gene flow. A phylogenetic analysis divided the populations into four clusters that correlated with the country of origin. The negligible gene flow between the Thai and Laos populations, despite sharing the Mekong Basin, caused us to reject our hypothesis. Our data suggest that the distribution of H. taichui populations was incidentally associated with national borders.
author2 Mahidol University
author_facet Mahidol University
U. Thaenkham
O. Phuphisut
S. Nuamtanong
T. Yoonuan
S. Sa-Nguankiat
Y. Vonghachack
V. Y. Belizario
D. T. Dung
P. Dekumyoy
J. Waikagul
format Article
author U. Thaenkham
O. Phuphisut
S. Nuamtanong
T. Yoonuan
S. Sa-Nguankiat
Y. Vonghachack
V. Y. Belizario
D. T. Dung
P. Dekumyoy
J. Waikagul
author_sort U. Thaenkham
title Genetic differences among Haplorchis taichui populations in Indochina revealed by mitochondrial COX1 sequences
title_short Genetic differences among Haplorchis taichui populations in Indochina revealed by mitochondrial COX1 sequences
title_full Genetic differences among Haplorchis taichui populations in Indochina revealed by mitochondrial COX1 sequences
title_fullStr Genetic differences among Haplorchis taichui populations in Indochina revealed by mitochondrial COX1 sequences
title_full_unstemmed Genetic differences among Haplorchis taichui populations in Indochina revealed by mitochondrial COX1 sequences
title_sort genetic differences among haplorchis taichui populations in indochina revealed by mitochondrial cox1 sequences
publishDate 2018
url https://repository.li.mahidol.ac.th/handle/123456789/41391
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