Evolution of Fseg/Cseg dimorphism in region III of the Plasmodium falciparum eba-175 gene
© 2017 Elsevier B.V. The 175-kDa erythrocyte binding antigen (EBA-175) of the malaria parasite Plasmodium falciparum is important for its invasion into human erythrocytes. The primary structure of eba-175 is divided into seven regions, namely I to VII. Region III contains highly divergent dimorphic...
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th-mahidol.415912019-03-14T15:02:33Z Evolution of Fseg/Cseg dimorphism in region III of the Plasmodium falciparum eba-175 gene Yoshiki Yasukochi Izumi Naka Jintana Patarapotikul Hathairad Hananantachai Jun Ohashi Mie University University of Tokyo Mahidol University Agricultural and Biological Sciences Biochemistry, Genetics and Molecular Biology Immunology and Microbiology © 2017 Elsevier B.V. The 175-kDa erythrocyte binding antigen (EBA-175) of the malaria parasite Plasmodium falciparum is important for its invasion into human erythrocytes. The primary structure of eba-175 is divided into seven regions, namely I to VII. Region III contains highly divergent dimorphic segments, termed Fseg and Cseg. The allele frequencies of segmental dimorphism within a P. falciparum population have been extensively examined; however, the molecular evolution of segmental dimorphism is not well understood. A comprehensive comparison of nucleotide sequences among 32 P. falciparum eba-175 alleles identified in our previous study, two Plasmodium reichenowi, and one P. gaboni orthologous alleles obtained from the GenBank database was conducted to uncover the origin and evolutionary processes of segmental dimorphism in P. falciparum eba-175. In the eba-175 nucleotide sequence derived from a P. reichenowi CDC strain, both Fseg and Cseg were found in region III, which implies that the original eba-175 gene had both segments, and deletions of F- and C-segments generated Cseg and Fseg alleles, respectively. We also confirmed the presence of allele with Fseg and Cseg in another P. reichenowi strain (SY57), by re-mapping short reads obtained from the GenBank database. On the other hand, the segmental sequence of eba-175 ortholog in P. gaboni was quite diverged from those of the other species, suggesting that the original eba-175 dimorphism of P. falciparum can be traced back to the stem linage of P. falciparum and P. reichenowi. Our findings suggest that Fseg and Cseg alleles are derived from a single eba-175 allele containing both segments in the ancestral population of P. falciparum and P. reichenowi, and that the allelic dimorphism of eba-175 was shaped by the independent emergence of similar dimorphic lineage in different species that has never been observed in any evolutionary mode of allelic dimorphism at other loci in malaria genomes. 2018-12-21T06:31:27Z 2019-03-14T08:02:33Z 2018-12-21T06:31:27Z 2019-03-14T08:02:33Z 2017-04-01 Article Infection, Genetics and Evolution. Vol.49, (2017), 251-255 10.1016/j.meegid.2017.01.026 15677257 15671348 2-s2.0-85011706120 https://repository.li.mahidol.ac.th/handle/123456789/41591 Mahidol University SCOPUS https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85011706120&origin=inward |
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Agricultural and Biological Sciences Biochemistry, Genetics and Molecular Biology Immunology and Microbiology Yoshiki Yasukochi Izumi Naka Jintana Patarapotikul Hathairad Hananantachai Jun Ohashi Evolution of Fseg/Cseg dimorphism in region III of the Plasmodium falciparum eba-175 gene |
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© 2017 Elsevier B.V. The 175-kDa erythrocyte binding antigen (EBA-175) of the malaria parasite Plasmodium falciparum is important for its invasion into human erythrocytes. The primary structure of eba-175 is divided into seven regions, namely I to VII. Region III contains highly divergent dimorphic segments, termed Fseg and Cseg. The allele frequencies of segmental dimorphism within a P. falciparum population have been extensively examined; however, the molecular evolution of segmental dimorphism is not well understood. A comprehensive comparison of nucleotide sequences among 32 P. falciparum eba-175 alleles identified in our previous study, two Plasmodium reichenowi, and one P. gaboni orthologous alleles obtained from the GenBank database was conducted to uncover the origin and evolutionary processes of segmental dimorphism in P. falciparum eba-175. In the eba-175 nucleotide sequence derived from a P. reichenowi CDC strain, both Fseg and Cseg were found in region III, which implies that the original eba-175 gene had both segments, and deletions of F- and C-segments generated Cseg and Fseg alleles, respectively. We also confirmed the presence of allele with Fseg and Cseg in another P. reichenowi strain (SY57), by re-mapping short reads obtained from the GenBank database. On the other hand, the segmental sequence of eba-175 ortholog in P. gaboni was quite diverged from those of the other species, suggesting that the original eba-175 dimorphism of P. falciparum can be traced back to the stem linage of P. falciparum and P. reichenowi. Our findings suggest that Fseg and Cseg alleles are derived from a single eba-175 allele containing both segments in the ancestral population of P. falciparum and P. reichenowi, and that the allelic dimorphism of eba-175 was shaped by the independent emergence of similar dimorphic lineage in different species that has never been observed in any evolutionary mode of allelic dimorphism at other loci in malaria genomes. |
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Mie University |
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Mie University Yoshiki Yasukochi Izumi Naka Jintana Patarapotikul Hathairad Hananantachai Jun Ohashi |
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Article |
author |
Yoshiki Yasukochi Izumi Naka Jintana Patarapotikul Hathairad Hananantachai Jun Ohashi |
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Yoshiki Yasukochi |
title |
Evolution of Fseg/Cseg dimorphism in region III of the Plasmodium falciparum eba-175 gene |
title_short |
Evolution of Fseg/Cseg dimorphism in region III of the Plasmodium falciparum eba-175 gene |
title_full |
Evolution of Fseg/Cseg dimorphism in region III of the Plasmodium falciparum eba-175 gene |
title_fullStr |
Evolution of Fseg/Cseg dimorphism in region III of the Plasmodium falciparum eba-175 gene |
title_full_unstemmed |
Evolution of Fseg/Cseg dimorphism in region III of the Plasmodium falciparum eba-175 gene |
title_sort |
evolution of fseg/cseg dimorphism in region iii of the plasmodium falciparum eba-175 gene |
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2018 |
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https://repository.li.mahidol.ac.th/handle/123456789/41591 |
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1763494600439234560 |