Identification and characterization of lbpA, an indigoidine biosynthetic gene in the γ-butyrolactone signaling system of Streptomyces lavendulae FRI-5

© 2017 The Society for Biotechnology, Japan Streptomyces lavendulae FRI-5 produces the blue pigment indigoidine and other secondary metabolites (D-cycloserine and nucleoside antibiotics). The production of these useful compounds is controlled by a signaling cascade mediated by the γ-butyrolactone au...

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Main Authors: Ivy Grace Umadhay Pait, Shigeru Kitani, Yohanes Novi Kurniawan, Maeda Asa, Takashi Iwai, Haruo Ikeda, Takuya Nihira
Other Authors: Osaka University
Format: Article
Published: 2018
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Online Access:https://repository.li.mahidol.ac.th/handle/123456789/41773
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spelling th-mahidol.417732019-03-14T15:02:46Z Identification and characterization of lbpA, an indigoidine biosynthetic gene in the γ-butyrolactone signaling system of Streptomyces lavendulae FRI-5 Ivy Grace Umadhay Pait Shigeru Kitani Yohanes Novi Kurniawan Maeda Asa Takashi Iwai Haruo Ikeda Takuya Nihira Osaka University Kitasato University Mahidol University Biochemistry, Genetics and Molecular Biology Chemical Engineering Immunology and Microbiology © 2017 The Society for Biotechnology, Japan Streptomyces lavendulae FRI-5 produces the blue pigment indigoidine and other secondary metabolites (D-cycloserine and nucleoside antibiotics). The production of these useful compounds is controlled by a signaling cascade mediated by the γ-butyrolactone autoregulator IM-2. Previously we revealed that the far regulatory island includes the IM-2 receptor, the IM-2 biosynthetic enzyme, and several transcriptional regulators, and that it contributes to the regulation of indigoidine production in response to the signaling molecule. Here, we found that the vicinity of the far regulatory island includes the putative gene cluster for the biosynthesis of indigoidine and unidentified compounds, and demonstrated that the expression of the gene cluster is under the control of the IM-2 regulatory system. Heterologous expression of lbpA, encoding a plausible nonribosomal peptide synthetase, in the versatile model host Streptomyces avermitilis SUKA22 led to indigoidine production, which was enhanced dramatically by feeding of the indigoidine precursor L-glutamine. These results confirmed that LbpA is an indigoidine biosynthetic enzyme in the IM-2 signaling cascade. 2018-12-21T06:41:41Z 2019-03-14T08:02:46Z 2018-12-21T06:41:41Z 2019-03-14T08:02:46Z 2017-10-01 Article Journal of Bioscience and Bioengineering. Vol.124, No.4 (2017), 369-375 10.1016/j.jbiosc.2017.04.020 13474421 13891723 2-s2.0-85019842634 https://repository.li.mahidol.ac.th/handle/123456789/41773 Mahidol University SCOPUS https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85019842634&origin=inward
institution Mahidol University
building Mahidol University Library
continent Asia
country Thailand
Thailand
content_provider Mahidol University Library
collection Mahidol University Institutional Repository
topic Biochemistry, Genetics and Molecular Biology
Chemical Engineering
Immunology and Microbiology
spellingShingle Biochemistry, Genetics and Molecular Biology
Chemical Engineering
Immunology and Microbiology
Ivy Grace Umadhay Pait
Shigeru Kitani
Yohanes Novi Kurniawan
Maeda Asa
Takashi Iwai
Haruo Ikeda
Takuya Nihira
Identification and characterization of lbpA, an indigoidine biosynthetic gene in the γ-butyrolactone signaling system of Streptomyces lavendulae FRI-5
description © 2017 The Society for Biotechnology, Japan Streptomyces lavendulae FRI-5 produces the blue pigment indigoidine and other secondary metabolites (D-cycloserine and nucleoside antibiotics). The production of these useful compounds is controlled by a signaling cascade mediated by the γ-butyrolactone autoregulator IM-2. Previously we revealed that the far regulatory island includes the IM-2 receptor, the IM-2 biosynthetic enzyme, and several transcriptional regulators, and that it contributes to the regulation of indigoidine production in response to the signaling molecule. Here, we found that the vicinity of the far regulatory island includes the putative gene cluster for the biosynthesis of indigoidine and unidentified compounds, and demonstrated that the expression of the gene cluster is under the control of the IM-2 regulatory system. Heterologous expression of lbpA, encoding a plausible nonribosomal peptide synthetase, in the versatile model host Streptomyces avermitilis SUKA22 led to indigoidine production, which was enhanced dramatically by feeding of the indigoidine precursor L-glutamine. These results confirmed that LbpA is an indigoidine biosynthetic enzyme in the IM-2 signaling cascade.
author2 Osaka University
author_facet Osaka University
Ivy Grace Umadhay Pait
Shigeru Kitani
Yohanes Novi Kurniawan
Maeda Asa
Takashi Iwai
Haruo Ikeda
Takuya Nihira
format Article
author Ivy Grace Umadhay Pait
Shigeru Kitani
Yohanes Novi Kurniawan
Maeda Asa
Takashi Iwai
Haruo Ikeda
Takuya Nihira
author_sort Ivy Grace Umadhay Pait
title Identification and characterization of lbpA, an indigoidine biosynthetic gene in the γ-butyrolactone signaling system of Streptomyces lavendulae FRI-5
title_short Identification and characterization of lbpA, an indigoidine biosynthetic gene in the γ-butyrolactone signaling system of Streptomyces lavendulae FRI-5
title_full Identification and characterization of lbpA, an indigoidine biosynthetic gene in the γ-butyrolactone signaling system of Streptomyces lavendulae FRI-5
title_fullStr Identification and characterization of lbpA, an indigoidine biosynthetic gene in the γ-butyrolactone signaling system of Streptomyces lavendulae FRI-5
title_full_unstemmed Identification and characterization of lbpA, an indigoidine biosynthetic gene in the γ-butyrolactone signaling system of Streptomyces lavendulae FRI-5
title_sort identification and characterization of lbpa, an indigoidine biosynthetic gene in the γ-butyrolactone signaling system of streptomyces lavendulae fri-5
publishDate 2018
url https://repository.li.mahidol.ac.th/handle/123456789/41773
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