An influenza A virus agglutination test using antibody-like polymers
© 2017 Informa UK Limited, trading as Taylor & Francis Group. Antibodies are commonly used in diagnostic routines to identify pathogens. The testing protocols are relatively simple, requiring a certain amount of a specific antibody to detect its corresponding pathogen. Antibody functionality c...
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th-mahidol.418232019-03-14T15:02:50Z An influenza A virus agglutination test using antibody-like polymers Wannisa Sukjee Arunee Thitithanyanont Suwimon Wiboon-ut Peter A. Lieberzeit M. Paul Gleeson Krongkaew Navakul Chak Sangma Kasetsart University Mahidol University Universitat Wien King Mongkut's Institute of Technology Ladkrabang Biochemistry, Genetics and Molecular Biology Chemical Engineering Engineering © 2017 Informa UK Limited, trading as Taylor & Francis Group. Antibodies are commonly used in diagnostic routines to identify pathogens. The testing protocols are relatively simple, requiring a certain amount of a specific antibody to detect its corresponding pathogen. Antibody functionality can be mimicked by synthesizing molecularly imprinted polymers (MIPs), i.e. polymers that can selectively recognize a given template structure. Thus, MIPs are sometimes termed ‘plastic antibody (PA)’. In this study, we have synthesized new granular MIPs using influenza A virus templates by precipitation polymerization. The selective binding of influenza A to the MIP particles was assessed and subsequently contrasted with other viruses. The affinities of influenza A virus towards the MIP was estimated based on an agglutination test by measuring the amount of influenza subtypes absorbed onto the MIPs. The MIPs produced using the H1N1 template showed specific reactivity to H1N1 while those produced using H5N1 and H3N2 templates showed cross-reactivity. 2018-12-21T06:40:33Z 2019-03-14T08:02:50Z 2018-12-21T06:40:33Z 2019-03-14T08:02:50Z 2017-10-13 Article Journal of Biomaterials Science, Polymer Edition. Vol.28, No.15 (2017), 1786-1795 10.1080/09205063.2017.1338503 15685624 09205063 2-s2.0-85020420899 https://repository.li.mahidol.ac.th/handle/123456789/41823 Mahidol University SCOPUS https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85020420899&origin=inward |
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Biochemistry, Genetics and Molecular Biology Chemical Engineering Engineering Wannisa Sukjee Arunee Thitithanyanont Suwimon Wiboon-ut Peter A. Lieberzeit M. Paul Gleeson Krongkaew Navakul Chak Sangma An influenza A virus agglutination test using antibody-like polymers |
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© 2017 Informa UK Limited, trading as Taylor & Francis Group. Antibodies are commonly used in diagnostic routines to identify pathogens. The testing protocols are relatively simple, requiring a certain amount of a specific antibody to detect its corresponding pathogen. Antibody functionality can be mimicked by synthesizing molecularly imprinted polymers (MIPs), i.e. polymers that can selectively recognize a given template structure. Thus, MIPs are sometimes termed ‘plastic antibody (PA)’. In this study, we have synthesized new granular MIPs using influenza A virus templates by precipitation polymerization. The selective binding of influenza A to the MIP particles was assessed and subsequently contrasted with other viruses. The affinities of influenza A virus towards the MIP was estimated based on an agglutination test by measuring the amount of influenza subtypes absorbed onto the MIPs. The MIPs produced using the H1N1 template showed specific reactivity to H1N1 while those produced using H5N1 and H3N2 templates showed cross-reactivity. |
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Kasetsart University |
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Kasetsart University Wannisa Sukjee Arunee Thitithanyanont Suwimon Wiboon-ut Peter A. Lieberzeit M. Paul Gleeson Krongkaew Navakul Chak Sangma |
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Article |
author |
Wannisa Sukjee Arunee Thitithanyanont Suwimon Wiboon-ut Peter A. Lieberzeit M. Paul Gleeson Krongkaew Navakul Chak Sangma |
author_sort |
Wannisa Sukjee |
title |
An influenza A virus agglutination test using antibody-like polymers |
title_short |
An influenza A virus agglutination test using antibody-like polymers |
title_full |
An influenza A virus agglutination test using antibody-like polymers |
title_fullStr |
An influenza A virus agglutination test using antibody-like polymers |
title_full_unstemmed |
An influenza A virus agglutination test using antibody-like polymers |
title_sort |
influenza a virus agglutination test using antibody-like polymers |
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2018 |
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https://repository.li.mahidol.ac.th/handle/123456789/41823 |
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1763491568950444032 |