A rhodol-based fluorescent chemosensor for hydrazine and its application in live cell bioimaging

• Main Authors: Khomsan Tiensomjitr, Rattha Noorat, Kanokorn Wechakorn, Samran Prabpai, Kanoknetr Suksen, Phongthon Kanjanasirirat, Yongyut Pewkliang, Suparerk Borwornpinyo, Palangpon Kongsaeree
• Other Authors: Mahidol University
• Format: Article
• Published: 2018
• Subjects: Chemistry
• Online Access: https://repository.li.mahidol.ac.th/handle/123456789/42183

© 2017 Elsevier B.V. A rhodol cinnamate fluorescent chemosensor (RC) has been developed for selective detection of hydrazine (N2H4). In aqueous medium, the rhodol-based probe exhibited high selectivity for hydrazine among other molecules. The addition of hydrazine triggered a fluorescence emission with 48-fold enhancement based on hydrazinolysis and a subsequent ring-opening process. The chemical probe also displayed a selective colorimetric response toward N2H4 from colorless solution to pink, readily observed by the naked eye. The detection limit of RC for hydrazine was calculated to be 300 nM (9.6 ppb). RC is membrane permeable and was successfully demonstrated to detect hydrazine in live HepG2 cells by confocal fluorescence microscopy.