Sex differences in the metabolic dysfunction and insulin resistance of skeletal muscle glucose transport following high fructose ingestion
© 2016 the American Physiological Society. The role of high fructose ingestion (HFI) in the development of conditions mimicking human metabolic syndrome has mostly been demonstrated in male animals; however, the extent of HFI-induced metabolic alterations in females remains unclear. The present stud...
Saved in:
Main Authors: | , , |
---|---|
Other Authors: | |
Format: | Article |
Published: |
2018
|
Subjects: | |
Online Access: | https://repository.li.mahidol.ac.th/handle/123456789/42281 |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
Institution: | Mahidol University |
id |
th-mahidol.42281 |
---|---|
record_format |
dspace |
spelling |
th-mahidol.422812019-03-14T15:03:20Z Sex differences in the metabolic dysfunction and insulin resistance of skeletal muscle glucose transport following high fructose ingestion Yupaporn Rattanavichit Natsasi Chukijrungroat Vitoon Saengsirisuwan Mahidol University Biochemistry, Genetics and Molecular Biology Medicine © 2016 the American Physiological Society. The role of high fructose ingestion (HFI) in the development of conditions mimicking human metabolic syndrome has mostly been demonstrated in male animals; however, the extent of HFI-induced metabolic alterations in females remains unclear. The present study investigated whether HFI-induced metabolic perturbations differ between sexes and whether HFI aggravates the metabolic disturbances under ovarian hormone deprivation. Male, female, and ovariectomized (OVX) Sprague-Dawley rats were given either water or liquid fructose (10% wt/vol) for 6 wk. Blood pressure, glucose tolerance, insulin-stimulated glucose transport activity and signaling proteins, including insulin receptor (IR), insulin receptor substrate 1 (IRS-1), Akt, Akt substrate of 160 kDa (AS160), AMPKα, JNK, p38 MAPK, angiotensin-converting enzyme (ACE), ANG II type 1 receptor (AT1R), ACE2, and Mas receptor (MasR) in skeletal muscle, were evaluated. We found that HFI led to glucose intolerance and hypertension in male and OVX rats but not in female rats with intact ovaries. Moreover, HFI did not induce insulin resistance in the skeletal muscle of female and OVX rats but impaired the insulin-stimulated glucose transport activity in the skeletal muscle of male rats, which was accompanied by lower insulin-stimulated IRS-1 Tyr989(44%), Akt Ser473(30%), and AS160 Ser588(43%), and increases in insulin-stimulated IRS-1 Ser307(78%), JNK Thr183/Tyr185(69%), and p38 MAPK Thr180/Tyr182(81%). The results from the present study show sex differences in the development of metabolic syndrome-like conditions and indicate the protective role of female sex hormones against HFI-induced cardiometabolic abnormalities. 2018-12-11T02:05:48Z 2019-03-14T08:03:20Z 2018-12-11T02:05:48Z 2019-03-14T08:03:20Z 2016-12-01 Article American Journal of Physiology - Regulatory Integrative and Comparative Physiology. Vol.311, No.6 (2016), R1200-R1212 10.1152/ajpregu.00230.2016 15221490 03636119 2-s2.0-85007227834 https://repository.li.mahidol.ac.th/handle/123456789/42281 Mahidol University SCOPUS https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85007227834&origin=inward |
institution |
Mahidol University |
building |
Mahidol University Library |
continent |
Asia |
country |
Thailand Thailand |
content_provider |
Mahidol University Library |
collection |
Mahidol University Institutional Repository |
topic |
Biochemistry, Genetics and Molecular Biology Medicine |
spellingShingle |
Biochemistry, Genetics and Molecular Biology Medicine Yupaporn Rattanavichit Natsasi Chukijrungroat Vitoon Saengsirisuwan Sex differences in the metabolic dysfunction and insulin resistance of skeletal muscle glucose transport following high fructose ingestion |
description |
© 2016 the American Physiological Society. The role of high fructose ingestion (HFI) in the development of conditions mimicking human metabolic syndrome has mostly been demonstrated in male animals; however, the extent of HFI-induced metabolic alterations in females remains unclear. The present study investigated whether HFI-induced metabolic perturbations differ between sexes and whether HFI aggravates the metabolic disturbances under ovarian hormone deprivation. Male, female, and ovariectomized (OVX) Sprague-Dawley rats were given either water or liquid fructose (10% wt/vol) for 6 wk. Blood pressure, glucose tolerance, insulin-stimulated glucose transport activity and signaling proteins, including insulin receptor (IR), insulin receptor substrate 1 (IRS-1), Akt, Akt substrate of 160 kDa (AS160), AMPKα, JNK, p38 MAPK, angiotensin-converting enzyme (ACE), ANG II type 1 receptor (AT1R), ACE2, and Mas receptor (MasR) in skeletal muscle, were evaluated. We found that HFI led to glucose intolerance and hypertension in male and OVX rats but not in female rats with intact ovaries. Moreover, HFI did not induce insulin resistance in the skeletal muscle of female and OVX rats but impaired the insulin-stimulated glucose transport activity in the skeletal muscle of male rats, which was accompanied by lower insulin-stimulated IRS-1 Tyr989(44%), Akt Ser473(30%), and AS160 Ser588(43%), and increases in insulin-stimulated IRS-1 Ser307(78%), JNK Thr183/Tyr185(69%), and p38 MAPK Thr180/Tyr182(81%). The results from the present study show sex differences in the development of metabolic syndrome-like conditions and indicate the protective role of female sex hormones against HFI-induced cardiometabolic abnormalities. |
author2 |
Mahidol University |
author_facet |
Mahidol University Yupaporn Rattanavichit Natsasi Chukijrungroat Vitoon Saengsirisuwan |
format |
Article |
author |
Yupaporn Rattanavichit Natsasi Chukijrungroat Vitoon Saengsirisuwan |
author_sort |
Yupaporn Rattanavichit |
title |
Sex differences in the metabolic dysfunction and insulin resistance of skeletal muscle glucose transport following high fructose ingestion |
title_short |
Sex differences in the metabolic dysfunction and insulin resistance of skeletal muscle glucose transport following high fructose ingestion |
title_full |
Sex differences in the metabolic dysfunction and insulin resistance of skeletal muscle glucose transport following high fructose ingestion |
title_fullStr |
Sex differences in the metabolic dysfunction and insulin resistance of skeletal muscle glucose transport following high fructose ingestion |
title_full_unstemmed |
Sex differences in the metabolic dysfunction and insulin resistance of skeletal muscle glucose transport following high fructose ingestion |
title_sort |
sex differences in the metabolic dysfunction and insulin resistance of skeletal muscle glucose transport following high fructose ingestion |
publishDate |
2018 |
url |
https://repository.li.mahidol.ac.th/handle/123456789/42281 |
_version_ |
1763498108561391616 |