Influence of N-methyl pyrrolidone on the activity of the pulp–dentine complex and bone integrity during osteoporosis
© 2016 International Endodontic Journal. Published by John Wiley & Sons Ltd Aim: To analyse the effect of systemic application of N-methyl pyrrolidone (NMP) on the pulp–dentine complex and on the jawbone of ovariectomized rats. Method: Female Sprague Dawley rats were randomly divided into a Sh...
Saved in:
Main Authors: | , , , , , |
---|---|
Other Authors: | |
Format: | Article |
Published: |
2018
|
Subjects: | |
Online Access: | https://repository.li.mahidol.ac.th/handle/123456789/42474 |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
Institution: | Mahidol University |
id |
th-mahidol.42474 |
---|---|
record_format |
dspace |
spelling |
th-mahidol.424742019-03-14T15:03:31Z Influence of N-methyl pyrrolidone on the activity of the pulp–dentine complex and bone integrity during osteoporosis B. Gjoksi N. Ruangsawasdi C. Ghayor B. Siegenthaler M. Zenobi-Wong F. E. Weber University of Zurich ETH Zurich Mahidol University Dentistry © 2016 International Endodontic Journal. Published by John Wiley & Sons Ltd Aim: To analyse the effect of systemic application of N-methyl pyrrolidone (NMP) on the pulp–dentine complex and on the jawbone of ovariectomized rats. Method: Female Sprague Dawley rats were randomly divided into a Sham-operated group (Sham n = 6) and an oestrogen depletion by ovariectomy (OVX n = 12) group. In 6 of the ovariectomized animals, N-methyl pyrrolidone (NMP) in phosphate-buffered saline (PBS) was administered systemically weekly by intraperitoneal injection (i.p.); the other 6 were injected with PBS (Veh). After 15 weeks of injections, the jaw bones were collected and pulps extracted from the incisors teeth. Histology was used to determine pre-dentine thickness in teeth and radiography to determine alveolar bone mass. Immunohistological staining and RT-PCR were performed to verify the presence and localization of the odontoblast-specific dentine sialoprotein and to quantify its expression in the dentine–pulp complex. Mandibular cortical width and mandibular height were evaluated by means of X-ray analysis. Statistical analysis was performed with analysis of variance (anova). Results: Both pre-dentine (P = 0.029) and alveolar bone structures (P = 0.049) were significantly reduced due to oestrogen deficiency in OVX Veh and OVX. NMP treatment normalized these parameters to the Sham level. DSPP expression in OVX NMP animals was significantly higher (P = 0.046) than in OVX Veh. X-ray analysis confirmed that ovariectomy significantly reduced the mandibular cortical width in the OVX Veh group compared to the Sham Veh and OVX NMP (P = 0.020). Conclusion: N-methyl pyrrolidone (NMP) had a remarkable anti-osteoporotic ability preserving activity in the pulp–dentine complex and preventing jawbone loss. These effects make NMP a promising candidate for the preservation of the activity of the pulp–dentine complex and jawbone thickness in post-menopausal females. 2018-12-21T07:25:50Z 2019-03-14T08:03:31Z 2018-12-21T07:25:50Z 2019-03-14T08:03:31Z 2017-03-01 Article International Endodontic Journal. Vol.50, No.3 (2017), 271-280 10.1111/iej.12622 13652591 01432885 2-s2.0-84963568630 https://repository.li.mahidol.ac.th/handle/123456789/42474 Mahidol University SCOPUS https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84963568630&origin=inward |
institution |
Mahidol University |
building |
Mahidol University Library |
continent |
Asia |
country |
Thailand Thailand |
content_provider |
Mahidol University Library |
collection |
Mahidol University Institutional Repository |
topic |
Dentistry |
spellingShingle |
Dentistry B. Gjoksi N. Ruangsawasdi C. Ghayor B. Siegenthaler M. Zenobi-Wong F. E. Weber Influence of N-methyl pyrrolidone on the activity of the pulp–dentine complex and bone integrity during osteoporosis |
description |
© 2016 International Endodontic Journal. Published by John Wiley & Sons Ltd Aim: To analyse the effect of systemic application of N-methyl pyrrolidone (NMP) on the pulp–dentine complex and on the jawbone of ovariectomized rats. Method: Female Sprague Dawley rats were randomly divided into a Sham-operated group (Sham n = 6) and an oestrogen depletion by ovariectomy (OVX n = 12) group. In 6 of the ovariectomized animals, N-methyl pyrrolidone (NMP) in phosphate-buffered saline (PBS) was administered systemically weekly by intraperitoneal injection (i.p.); the other 6 were injected with PBS (Veh). After 15 weeks of injections, the jaw bones were collected and pulps extracted from the incisors teeth. Histology was used to determine pre-dentine thickness in teeth and radiography to determine alveolar bone mass. Immunohistological staining and RT-PCR were performed to verify the presence and localization of the odontoblast-specific dentine sialoprotein and to quantify its expression in the dentine–pulp complex. Mandibular cortical width and mandibular height were evaluated by means of X-ray analysis. Statistical analysis was performed with analysis of variance (anova). Results: Both pre-dentine (P = 0.029) and alveolar bone structures (P = 0.049) were significantly reduced due to oestrogen deficiency in OVX Veh and OVX. NMP treatment normalized these parameters to the Sham level. DSPP expression in OVX NMP animals was significantly higher (P = 0.046) than in OVX Veh. X-ray analysis confirmed that ovariectomy significantly reduced the mandibular cortical width in the OVX Veh group compared to the Sham Veh and OVX NMP (P = 0.020). Conclusion: N-methyl pyrrolidone (NMP) had a remarkable anti-osteoporotic ability preserving activity in the pulp–dentine complex and preventing jawbone loss. These effects make NMP a promising candidate for the preservation of the activity of the pulp–dentine complex and jawbone thickness in post-menopausal females. |
author2 |
University of Zurich |
author_facet |
University of Zurich B. Gjoksi N. Ruangsawasdi C. Ghayor B. Siegenthaler M. Zenobi-Wong F. E. Weber |
format |
Article |
author |
B. Gjoksi N. Ruangsawasdi C. Ghayor B. Siegenthaler M. Zenobi-Wong F. E. Weber |
author_sort |
B. Gjoksi |
title |
Influence of N-methyl pyrrolidone on the activity of the pulp–dentine complex and bone integrity during osteoporosis |
title_short |
Influence of N-methyl pyrrolidone on the activity of the pulp–dentine complex and bone integrity during osteoporosis |
title_full |
Influence of N-methyl pyrrolidone on the activity of the pulp–dentine complex and bone integrity during osteoporosis |
title_fullStr |
Influence of N-methyl pyrrolidone on the activity of the pulp–dentine complex and bone integrity during osteoporosis |
title_full_unstemmed |
Influence of N-methyl pyrrolidone on the activity of the pulp–dentine complex and bone integrity during osteoporosis |
title_sort |
influence of n-methyl pyrrolidone on the activity of the pulp–dentine complex and bone integrity during osteoporosis |
publishDate |
2018 |
url |
https://repository.li.mahidol.ac.th/handle/123456789/42474 |
_version_ |
1763491201513684992 |