Adhesion of Candida to vanillin-coated PMMA resin

• Main Authors: Sroisiri Thaweboon, Boonyanit Thaweboon, Rattiporn Kaypetch
• Other Authors: Mahidol University
• Format: Conference or Workshop Item
• Published: 2018
• Subjects: Engineering
• Online Access: https://repository.li.mahidol.ac.th/handle/123456789/42634

© 2017 Trans Tech Publications, Switzerland. Denture stomatitis is a common clinical disorder seen among denture wearers. Candida albicans is considered to be the major cause which can adhere to and colonize the surface of denture base material made of polymethyl methacrylate (PMMA) resin. This study aims to investigate the effect of vanillin-coated PMMA resin on the adhesion of C. albicans. Heat-polymerized PMMA resin (Vertex RS, Dentimex, Netherlands) samples were prepared and coated with vanillin at various concentrations of 7, 11, and 15 mM. Sterile distilled water and 0.2% chlorhexidine gluconate solution were used to coat the surfaces in negative and positive controls, respectively. The adhesion assay was performed with 3 strains of C. albicans based on the method described by Samaranayake and MacFarlane. The numbers of adherent yeast cells on PMMA resin surfaces were counted under microscope by random selection of 20 fields at 400x magnification. It was found that all vanillincoated PMMA resin samples had significant decrease of candida adhesion (50-90% reduction) compared to the negative control. The effect was observed to be in a dose dependent manner (p<0.05). Chlorhexidine exhibited strong reduction effect (96-98% reduction). In conclusion, coating PMMA resin with 7-15 mM vanillin can inhibit candida adhesion to the surface. With further testing and development, vanillin may be used as coating agent for denture to prevent candida adhesion and reduce risk of denture stomatitis.