Genetic polymorphisms and allergenicity of blo t 5 in a house dust mite allergic filipino population

© 2017, Allergy and Immunology Society of Thailand. All rights reserved. Background: Blo t 5, a major allergen from the house dust mite Blomia tropicalis had been identified as an important trigger of allergic diseases. Effects of sequence variations to the IgE binding activity of Blo t 5 remain unc...

Full description

Saved in:
Bibliographic Details
Main Authors: Loida R. Medina, Nat Malainual, John Donnie A. Ramos
Other Authors: University of Santo Tomas, Manila
Format: Article
Published: 2018
Subjects:
Online Access:https://repository.li.mahidol.ac.th/handle/123456789/42708
Tags: Add Tag
No Tags, Be the first to tag this record!
Institution: Mahidol University
id th-mahidol.42708
record_format dspace
spelling th-mahidol.427082019-03-14T15:03:44Z Genetic polymorphisms and allergenicity of blo t 5 in a house dust mite allergic filipino population Loida R. Medina Nat Malainual John Donnie A. Ramos University of Santo Tomas, Manila Mahidol University Immunology and Microbiology © 2017, Allergy and Immunology Society of Thailand. All rights reserved. Background: Blo t 5, a major allergen from the house dust mite Blomia tropicalis had been identified as an important trigger of allergic diseases. Effects of sequence variations to the IgE binding activity of Blo t 5 remain unclear. Objective: The study determined gene polymorphisms of Blo t 5, expressed the recombinant Blo t 5 allergen in E. coli system and determined its IgE reactivity in house dust mite allergic Filipino patients. Method: Gene polymorphism was determined using Polymerase Chain Reaction and DNA sequencing. Recombinant Blo t 5 was expressed as a GST-fusion protein in E. coli and purified by affinity chromatography. IgE-binding activity of 230 age- and sex-matched allergic and non-allergic subjects was subjected to Enzyme-linked Immunosorbent Assay and Western blot analysis. Results: Five polymorphic variants of Blo t 5 were identified with 26 DNA base and 12 amino acid substitutions thus implying a high degree of sequence diversity. Of the 115 cases, 68.70% and 59.13% showed reactivity to Blo t extract and rBlo t 5 respectively. Total and specific IgE levels of allergic and non-allergic subjects differ significantly (p<0.0001). Conclusions: Blo t 5 gene exhibits polymorphic variants with predicted amino acid sequences resulting to changes in its IgE epitopes. These polymorphisms may suggest variability of allergenic properties of Blo t 5. The incorporation of the Blo t 5 variants in the panel of diagnostic allergens and immunotherapy is highly recommended. 2018-12-21T07:50:19Z 2019-03-14T08:03:44Z 2018-12-21T07:50:19Z 2019-03-14T08:03:44Z 2017-12-01 Article Asian Pacific Journal of Allergy and Immunology. Vol.35, No.4 (2017), 203-211 10.12932/AP0794 22288694 0125877X 2-s2.0-85041518808 https://repository.li.mahidol.ac.th/handle/123456789/42708 Mahidol University SCOPUS https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85041518808&origin=inward
institution Mahidol University
building Mahidol University Library
continent Asia
country Thailand
Thailand
content_provider Mahidol University Library
collection Mahidol University Institutional Repository
topic Immunology and Microbiology
spellingShingle Immunology and Microbiology
Loida R. Medina
Nat Malainual
John Donnie A. Ramos
Genetic polymorphisms and allergenicity of blo t 5 in a house dust mite allergic filipino population
description © 2017, Allergy and Immunology Society of Thailand. All rights reserved. Background: Blo t 5, a major allergen from the house dust mite Blomia tropicalis had been identified as an important trigger of allergic diseases. Effects of sequence variations to the IgE binding activity of Blo t 5 remain unclear. Objective: The study determined gene polymorphisms of Blo t 5, expressed the recombinant Blo t 5 allergen in E. coli system and determined its IgE reactivity in house dust mite allergic Filipino patients. Method: Gene polymorphism was determined using Polymerase Chain Reaction and DNA sequencing. Recombinant Blo t 5 was expressed as a GST-fusion protein in E. coli and purified by affinity chromatography. IgE-binding activity of 230 age- and sex-matched allergic and non-allergic subjects was subjected to Enzyme-linked Immunosorbent Assay and Western blot analysis. Results: Five polymorphic variants of Blo t 5 were identified with 26 DNA base and 12 amino acid substitutions thus implying a high degree of sequence diversity. Of the 115 cases, 68.70% and 59.13% showed reactivity to Blo t extract and rBlo t 5 respectively. Total and specific IgE levels of allergic and non-allergic subjects differ significantly (p<0.0001). Conclusions: Blo t 5 gene exhibits polymorphic variants with predicted amino acid sequences resulting to changes in its IgE epitopes. These polymorphisms may suggest variability of allergenic properties of Blo t 5. The incorporation of the Blo t 5 variants in the panel of diagnostic allergens and immunotherapy is highly recommended.
author2 University of Santo Tomas, Manila
author_facet University of Santo Tomas, Manila
Loida R. Medina
Nat Malainual
John Donnie A. Ramos
format Article
author Loida R. Medina
Nat Malainual
John Donnie A. Ramos
author_sort Loida R. Medina
title Genetic polymorphisms and allergenicity of blo t 5 in a house dust mite allergic filipino population
title_short Genetic polymorphisms and allergenicity of blo t 5 in a house dust mite allergic filipino population
title_full Genetic polymorphisms and allergenicity of blo t 5 in a house dust mite allergic filipino population
title_fullStr Genetic polymorphisms and allergenicity of blo t 5 in a house dust mite allergic filipino population
title_full_unstemmed Genetic polymorphisms and allergenicity of blo t 5 in a house dust mite allergic filipino population
title_sort genetic polymorphisms and allergenicity of blo t 5 in a house dust mite allergic filipino population
publishDate 2018
url https://repository.li.mahidol.ac.th/handle/123456789/42708
_version_ 1763490971817869312