Quinolone Resistance Determinants of Clinical Salmonella Enteritidis in Thailand

© Copyright 2017, Mary Ann Liebert, Inc. 2017. Salmonella Enteritidis has emerged as a global concern regarding quinolone resistance and invasive potential. Although quinolone-resistant S. Enteritidis has been observed with high frequency in Thailand, information on the mechanism of resistance acqui...

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Main Authors: Fuangfa Utrarachkij, Chie Nakajima, Ruchirada Changkwanyeun, Kanokrat Siripanichgon, Siriporn Kongsoi, Srirat Pornruangwong, Kanjana Changkaew, Risa Tsunoda, Yutaka Tamura, Orasa Suthienkul, Yasuhiko Suzuki
Other Authors: Mahidol University
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Published: 2018
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Online Access:https://repository.li.mahidol.ac.th/handle/123456789/42749
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spelling th-mahidol.427492019-03-14T15:03:46Z Quinolone Resistance Determinants of Clinical Salmonella Enteritidis in Thailand Fuangfa Utrarachkij Chie Nakajima Ruchirada Changkwanyeun Kanokrat Siripanichgon Siriporn Kongsoi Srirat Pornruangwong Kanjana Changkaew Risa Tsunoda Yutaka Tamura Orasa Suthienkul Yasuhiko Suzuki Mahidol University Hokkaido University National Institutes of Health, Bethesda Thammasat University Rakuno Gakuen University Immunology and Microbiology © Copyright 2017, Mary Ann Liebert, Inc. 2017. Salmonella Enteritidis has emerged as a global concern regarding quinolone resistance and invasive potential. Although quinolone-resistant S. Enteritidis has been observed with high frequency in Thailand, information on the mechanism of resistance acquisition is limited. To elucidate the mechanism, a total of 158 clinical isolates of nalidixic acid (NAL)-resistant S. Enteritidis were collected throughout Thailand, and the quinolone resistance determinants were investigated in the context of resistance levels to NAL, norfloxacin (NOR), and ciprofloxacin (CIP). The analysis of point mutations in type II topoisomerase genes and the detection of plasmid-mediated quinolone resistance genes showed that all but two harbored a gyrA mutation, the qnrS1 gene, or both. The most commonly affected codon in mutant gyrA was 87, followed by 83. Double codon mutation in gyrA was found in an isolate with high-level resistance to NAL, NOR, and CIP. A new mutation causing serine to isoleucine substitution at codon 83 was identified in eight isolates. In addition to eighteen qnrS1-carrying isolates showing nontypical quinolone resistance, one carrying both the qnrS1 gene and a gyrA mutation also showed a high level of resistance. Genotyping by multilocus variable number of tandem repeat analysis suggested a possible clonal expansion of NAL-resistant strains nationwide. Our data suggested that NAL-resistant isolates with single quinolone resistance determinant may potentially become fluoroquinolone resistant by acquiring secondary determinants. Restricted therapeutic and farming usage of quinolones is strongly recommended to prevent the emergence of fluoroquinolone-resistant isolates. 2018-12-21T07:53:12Z 2019-03-14T08:03:46Z 2018-12-21T07:53:12Z 2019-03-14T08:03:46Z 2017-10-01 Article Microbial Drug Resistance. Vol.23, No.7 (2017), 885-894 10.1089/mdr.2015.0234 19318448 10766294 2-s2.0-85031288388 https://repository.li.mahidol.ac.th/handle/123456789/42749 Mahidol University SCOPUS https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85031288388&origin=inward
institution Mahidol University
building Mahidol University Library
continent Asia
country Thailand
Thailand
content_provider Mahidol University Library
collection Mahidol University Institutional Repository
topic Immunology and Microbiology
spellingShingle Immunology and Microbiology
Fuangfa Utrarachkij
Chie Nakajima
Ruchirada Changkwanyeun
Kanokrat Siripanichgon
Siriporn Kongsoi
Srirat Pornruangwong
Kanjana Changkaew
Risa Tsunoda
Yutaka Tamura
Orasa Suthienkul
Yasuhiko Suzuki
Quinolone Resistance Determinants of Clinical Salmonella Enteritidis in Thailand
description © Copyright 2017, Mary Ann Liebert, Inc. 2017. Salmonella Enteritidis has emerged as a global concern regarding quinolone resistance and invasive potential. Although quinolone-resistant S. Enteritidis has been observed with high frequency in Thailand, information on the mechanism of resistance acquisition is limited. To elucidate the mechanism, a total of 158 clinical isolates of nalidixic acid (NAL)-resistant S. Enteritidis were collected throughout Thailand, and the quinolone resistance determinants were investigated in the context of resistance levels to NAL, norfloxacin (NOR), and ciprofloxacin (CIP). The analysis of point mutations in type II topoisomerase genes and the detection of plasmid-mediated quinolone resistance genes showed that all but two harbored a gyrA mutation, the qnrS1 gene, or both. The most commonly affected codon in mutant gyrA was 87, followed by 83. Double codon mutation in gyrA was found in an isolate with high-level resistance to NAL, NOR, and CIP. A new mutation causing serine to isoleucine substitution at codon 83 was identified in eight isolates. In addition to eighteen qnrS1-carrying isolates showing nontypical quinolone resistance, one carrying both the qnrS1 gene and a gyrA mutation also showed a high level of resistance. Genotyping by multilocus variable number of tandem repeat analysis suggested a possible clonal expansion of NAL-resistant strains nationwide. Our data suggested that NAL-resistant isolates with single quinolone resistance determinant may potentially become fluoroquinolone resistant by acquiring secondary determinants. Restricted therapeutic and farming usage of quinolones is strongly recommended to prevent the emergence of fluoroquinolone-resistant isolates.
author2 Mahidol University
author_facet Mahidol University
Fuangfa Utrarachkij
Chie Nakajima
Ruchirada Changkwanyeun
Kanokrat Siripanichgon
Siriporn Kongsoi
Srirat Pornruangwong
Kanjana Changkaew
Risa Tsunoda
Yutaka Tamura
Orasa Suthienkul
Yasuhiko Suzuki
format Article
author Fuangfa Utrarachkij
Chie Nakajima
Ruchirada Changkwanyeun
Kanokrat Siripanichgon
Siriporn Kongsoi
Srirat Pornruangwong
Kanjana Changkaew
Risa Tsunoda
Yutaka Tamura
Orasa Suthienkul
Yasuhiko Suzuki
author_sort Fuangfa Utrarachkij
title Quinolone Resistance Determinants of Clinical Salmonella Enteritidis in Thailand
title_short Quinolone Resistance Determinants of Clinical Salmonella Enteritidis in Thailand
title_full Quinolone Resistance Determinants of Clinical Salmonella Enteritidis in Thailand
title_fullStr Quinolone Resistance Determinants of Clinical Salmonella Enteritidis in Thailand
title_full_unstemmed Quinolone Resistance Determinants of Clinical Salmonella Enteritidis in Thailand
title_sort quinolone resistance determinants of clinical salmonella enteritidis in thailand
publishDate 2018
url https://repository.li.mahidol.ac.th/handle/123456789/42749
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