Quinolone Resistance Determinants of Clinical Salmonella Enteritidis in Thailand
© Copyright 2017, Mary Ann Liebert, Inc. 2017. Salmonella Enteritidis has emerged as a global concern regarding quinolone resistance and invasive potential. Although quinolone-resistant S. Enteritidis has been observed with high frequency in Thailand, information on the mechanism of resistance acqui...
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th-mahidol.427492019-03-14T15:03:46Z Quinolone Resistance Determinants of Clinical Salmonella Enteritidis in Thailand Fuangfa Utrarachkij Chie Nakajima Ruchirada Changkwanyeun Kanokrat Siripanichgon Siriporn Kongsoi Srirat Pornruangwong Kanjana Changkaew Risa Tsunoda Yutaka Tamura Orasa Suthienkul Yasuhiko Suzuki Mahidol University Hokkaido University National Institutes of Health, Bethesda Thammasat University Rakuno Gakuen University Immunology and Microbiology © Copyright 2017, Mary Ann Liebert, Inc. 2017. Salmonella Enteritidis has emerged as a global concern regarding quinolone resistance and invasive potential. Although quinolone-resistant S. Enteritidis has been observed with high frequency in Thailand, information on the mechanism of resistance acquisition is limited. To elucidate the mechanism, a total of 158 clinical isolates of nalidixic acid (NAL)-resistant S. Enteritidis were collected throughout Thailand, and the quinolone resistance determinants were investigated in the context of resistance levels to NAL, norfloxacin (NOR), and ciprofloxacin (CIP). The analysis of point mutations in type II topoisomerase genes and the detection of plasmid-mediated quinolone resistance genes showed that all but two harbored a gyrA mutation, the qnrS1 gene, or both. The most commonly affected codon in mutant gyrA was 87, followed by 83. Double codon mutation in gyrA was found in an isolate with high-level resistance to NAL, NOR, and CIP. A new mutation causing serine to isoleucine substitution at codon 83 was identified in eight isolates. In addition to eighteen qnrS1-carrying isolates showing nontypical quinolone resistance, one carrying both the qnrS1 gene and a gyrA mutation also showed a high level of resistance. Genotyping by multilocus variable number of tandem repeat analysis suggested a possible clonal expansion of NAL-resistant strains nationwide. Our data suggested that NAL-resistant isolates with single quinolone resistance determinant may potentially become fluoroquinolone resistant by acquiring secondary determinants. Restricted therapeutic and farming usage of quinolones is strongly recommended to prevent the emergence of fluoroquinolone-resistant isolates. 2018-12-21T07:53:12Z 2019-03-14T08:03:46Z 2018-12-21T07:53:12Z 2019-03-14T08:03:46Z 2017-10-01 Article Microbial Drug Resistance. Vol.23, No.7 (2017), 885-894 10.1089/mdr.2015.0234 19318448 10766294 2-s2.0-85031288388 https://repository.li.mahidol.ac.th/handle/123456789/42749 Mahidol University SCOPUS https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85031288388&origin=inward |
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Immunology and Microbiology Fuangfa Utrarachkij Chie Nakajima Ruchirada Changkwanyeun Kanokrat Siripanichgon Siriporn Kongsoi Srirat Pornruangwong Kanjana Changkaew Risa Tsunoda Yutaka Tamura Orasa Suthienkul Yasuhiko Suzuki Quinolone Resistance Determinants of Clinical Salmonella Enteritidis in Thailand |
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© Copyright 2017, Mary Ann Liebert, Inc. 2017. Salmonella Enteritidis has emerged as a global concern regarding quinolone resistance and invasive potential. Although quinolone-resistant S. Enteritidis has been observed with high frequency in Thailand, information on the mechanism of resistance acquisition is limited. To elucidate the mechanism, a total of 158 clinical isolates of nalidixic acid (NAL)-resistant S. Enteritidis were collected throughout Thailand, and the quinolone resistance determinants were investigated in the context of resistance levels to NAL, norfloxacin (NOR), and ciprofloxacin (CIP). The analysis of point mutations in type II topoisomerase genes and the detection of plasmid-mediated quinolone resistance genes showed that all but two harbored a gyrA mutation, the qnrS1 gene, or both. The most commonly affected codon in mutant gyrA was 87, followed by 83. Double codon mutation in gyrA was found in an isolate with high-level resistance to NAL, NOR, and CIP. A new mutation causing serine to isoleucine substitution at codon 83 was identified in eight isolates. In addition to eighteen qnrS1-carrying isolates showing nontypical quinolone resistance, one carrying both the qnrS1 gene and a gyrA mutation also showed a high level of resistance. Genotyping by multilocus variable number of tandem repeat analysis suggested a possible clonal expansion of NAL-resistant strains nationwide. Our data suggested that NAL-resistant isolates with single quinolone resistance determinant may potentially become fluoroquinolone resistant by acquiring secondary determinants. Restricted therapeutic and farming usage of quinolones is strongly recommended to prevent the emergence of fluoroquinolone-resistant isolates. |
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Mahidol University |
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Mahidol University Fuangfa Utrarachkij Chie Nakajima Ruchirada Changkwanyeun Kanokrat Siripanichgon Siriporn Kongsoi Srirat Pornruangwong Kanjana Changkaew Risa Tsunoda Yutaka Tamura Orasa Suthienkul Yasuhiko Suzuki |
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Article |
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Fuangfa Utrarachkij Chie Nakajima Ruchirada Changkwanyeun Kanokrat Siripanichgon Siriporn Kongsoi Srirat Pornruangwong Kanjana Changkaew Risa Tsunoda Yutaka Tamura Orasa Suthienkul Yasuhiko Suzuki |
author_sort |
Fuangfa Utrarachkij |
title |
Quinolone Resistance Determinants of Clinical Salmonella Enteritidis in Thailand |
title_short |
Quinolone Resistance Determinants of Clinical Salmonella Enteritidis in Thailand |
title_full |
Quinolone Resistance Determinants of Clinical Salmonella Enteritidis in Thailand |
title_fullStr |
Quinolone Resistance Determinants of Clinical Salmonella Enteritidis in Thailand |
title_full_unstemmed |
Quinolone Resistance Determinants of Clinical Salmonella Enteritidis in Thailand |
title_sort |
quinolone resistance determinants of clinical salmonella enteritidis in thailand |
publishDate |
2018 |
url |
https://repository.li.mahidol.ac.th/handle/123456789/42749 |
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1763489524445347840 |