Effects of Thai piperaceae plant extracts on Neospora caninum infection

© 2017 Elsevier B.V. Neosporosis has a worldwide distribution and causes economic losses in farming, particularly by increasing the risk of abortion in cattle. This study investigated the effects of Thai piperaceae (Piper betle, P. nigrum, and P. sarmentosum) extracts on Neospora caninum infections...

Full description

Saved in:
Bibliographic Details
Main Authors: Arpron Leesombun, Sookruetai Boonmasawai, Yoshifumi Nishikawa
Other Authors: Obihiro University of Agriculture and Veterinary Medicine
Format: Article
Published: 2018
Subjects:
Online Access:https://repository.li.mahidol.ac.th/handle/123456789/42799
Tags: Add Tag
No Tags, Be the first to tag this record!
Institution: Mahidol University
id th-mahidol.42799
record_format dspace
spelling th-mahidol.427992019-03-14T15:03:50Z Effects of Thai piperaceae plant extracts on Neospora caninum infection Arpron Leesombun Sookruetai Boonmasawai Yoshifumi Nishikawa Obihiro University of Agriculture and Veterinary Medicine Mahidol University Immunology and Microbiology © 2017 Elsevier B.V. Neosporosis has a worldwide distribution and causes economic losses in farming, particularly by increasing the risk of abortion in cattle. This study investigated the effects of Thai piperaceae (Piper betle, P. nigrum, and P. sarmentosum) extracts on Neospora caninum infections in vitro and in vivo. In an in vitro parasite growth assay based on the green fluorescent protein (GFP) signal, P. betle was the most effective extract at inhibiting parasite growth in human foreskin fibroblast cells (IC50of GFP-expressing N. caninum parasites, 22.1 μg/ml). The P. betle extract, at 25 μg per ml, inhibited parasite invasion into host cells. Furthermore, in two independent experiments, treating N. caninum-infected mice with the P. betle extract for 7 days post-infection increased their survival. In trial one, the anti-N. caninum effects of the P. betle extract reduced the mouse clinical scores for 30 days post-infection (dpi). The survival rate of the mice treated with 400 mg/kg was 100% compared with 66.6% for those treated with 100 mg/kg and the non-treated controls. In trial two, treating the infected mice with the P. betle extract increased their survival at 50 dpi. All mice in the non-treatment group died; however, the survival rates of the 400 mg/kg-treated and 100 mg/kg-treated mice were 83.3% and 33.3%, respectively. Also, a trend towards a reduced parasite burden was noted in the brains of the P. betle extract-treated mice, compared with the control mice. Therefore P. betle extract has potential as a medicinal plant for treating neosporosis. 2018-12-21T07:57:29Z 2019-03-14T08:03:50Z 2018-12-21T07:57:29Z 2019-03-14T08:03:50Z 2017-06-01 Article Parasitology International. Vol.66, No.3 (2017), 219-226 10.1016/j.parint.2017.01.017 18730329 13835769 2-s2.0-85011860350 https://repository.li.mahidol.ac.th/handle/123456789/42799 Mahidol University SCOPUS https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85011860350&origin=inward
institution Mahidol University
building Mahidol University Library
continent Asia
country Thailand
Thailand
content_provider Mahidol University Library
collection Mahidol University Institutional Repository
topic Immunology and Microbiology
spellingShingle Immunology and Microbiology
Arpron Leesombun
Sookruetai Boonmasawai
Yoshifumi Nishikawa
Effects of Thai piperaceae plant extracts on Neospora caninum infection
description © 2017 Elsevier B.V. Neosporosis has a worldwide distribution and causes economic losses in farming, particularly by increasing the risk of abortion in cattle. This study investigated the effects of Thai piperaceae (Piper betle, P. nigrum, and P. sarmentosum) extracts on Neospora caninum infections in vitro and in vivo. In an in vitro parasite growth assay based on the green fluorescent protein (GFP) signal, P. betle was the most effective extract at inhibiting parasite growth in human foreskin fibroblast cells (IC50of GFP-expressing N. caninum parasites, 22.1 μg/ml). The P. betle extract, at 25 μg per ml, inhibited parasite invasion into host cells. Furthermore, in two independent experiments, treating N. caninum-infected mice with the P. betle extract for 7 days post-infection increased their survival. In trial one, the anti-N. caninum effects of the P. betle extract reduced the mouse clinical scores for 30 days post-infection (dpi). The survival rate of the mice treated with 400 mg/kg was 100% compared with 66.6% for those treated with 100 mg/kg and the non-treated controls. In trial two, treating the infected mice with the P. betle extract increased their survival at 50 dpi. All mice in the non-treatment group died; however, the survival rates of the 400 mg/kg-treated and 100 mg/kg-treated mice were 83.3% and 33.3%, respectively. Also, a trend towards a reduced parasite burden was noted in the brains of the P. betle extract-treated mice, compared with the control mice. Therefore P. betle extract has potential as a medicinal plant for treating neosporosis.
author2 Obihiro University of Agriculture and Veterinary Medicine
author_facet Obihiro University of Agriculture and Veterinary Medicine
Arpron Leesombun
Sookruetai Boonmasawai
Yoshifumi Nishikawa
format Article
author Arpron Leesombun
Sookruetai Boonmasawai
Yoshifumi Nishikawa
author_sort Arpron Leesombun
title Effects of Thai piperaceae plant extracts on Neospora caninum infection
title_short Effects of Thai piperaceae plant extracts on Neospora caninum infection
title_full Effects of Thai piperaceae plant extracts on Neospora caninum infection
title_fullStr Effects of Thai piperaceae plant extracts on Neospora caninum infection
title_full_unstemmed Effects of Thai piperaceae plant extracts on Neospora caninum infection
title_sort effects of thai piperaceae plant extracts on neospora caninum infection
publishDate 2018
url https://repository.li.mahidol.ac.th/handle/123456789/42799
_version_ 1763498223249391616