Utility of Japanese encephalitis virus subgenomic replicon-based single-round infectious particles as antigens in neutralization tests for Zika virus and three other flaviviruses
© 2017 Elsevier B.V. The introduction of a foreign virus into an area may cause an outbreak, as with the Zika virus (ZIKV) outbreak in the Americas. Preparedness for handling a viral outbreak involves the development of tests for the serodiagnosis of foreign virus infections. We previously establish...
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th-mahidol.428112019-03-14T15:03:50Z Utility of Japanese encephalitis virus subgenomic replicon-based single-round infectious particles as antigens in neutralization tests for Zika virus and three other flaviviruses Atsushi Yamanaka Meng Ling Moi Tomohiko Takasaki Ichiro Kurane Mami Matsuda Ryosuke Suzuki Eiji Konishi Mahidol University Osaka University National Institute of Infectious Diseases Nagasaki University Kanagawa Prefectural Institute of Public Health Immunology and Microbiology © 2017 Elsevier B.V. The introduction of a foreign virus into an area may cause an outbreak, as with the Zika virus (ZIKV) outbreak in the Americas. Preparedness for handling a viral outbreak involves the development of tests for the serodiagnosis of foreign virus infections. We previously established a gene-based technology to generate some flaviviral antigens useful for functional antibody assays. The technology utilizes a Japanese encephalitis virus subgenomic replicon to generate single-round infectious particles (SRIPs) that possess designed surface antigens. In the present study, we successfully expanded the capacity of SRIPs to four human-pathogenic mosquito-borne flaviviruses that could potentially be introduced from endemic to non-endemic countries: ZIKV, Sepik virus, Wesselsbron virus, and Usutu virus. Flavivirus-crossreactive monoclonal antibodies dose-dependently neutralized these SRIPs. ZIKV-SRIPs also produced antibody-dose-dependent neutralization curves equivalent to those shown by authentic ZIKV particles using sera from a Zika fever patient. The faithful expression of designed surface antigens on SRIPs will allow their use in neutralization tests to diagnose foreign flaviviral infections. 2018-12-21T07:58:54Z 2019-03-14T08:03:50Z 2018-12-21T07:58:54Z 2019-03-14T08:03:50Z 2017-05-01 Article Journal of Virological Methods. Vol.243, (2017), 164-171 10.1016/j.jviromet.2017.02.011 18790984 01660934 2-s2.0-85014052708 https://repository.li.mahidol.ac.th/handle/123456789/42811 Mahidol University SCOPUS https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85014052708&origin=inward |
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Immunology and Microbiology Atsushi Yamanaka Meng Ling Moi Tomohiko Takasaki Ichiro Kurane Mami Matsuda Ryosuke Suzuki Eiji Konishi Utility of Japanese encephalitis virus subgenomic replicon-based single-round infectious particles as antigens in neutralization tests for Zika virus and three other flaviviruses |
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© 2017 Elsevier B.V. The introduction of a foreign virus into an area may cause an outbreak, as with the Zika virus (ZIKV) outbreak in the Americas. Preparedness for handling a viral outbreak involves the development of tests for the serodiagnosis of foreign virus infections. We previously established a gene-based technology to generate some flaviviral antigens useful for functional antibody assays. The technology utilizes a Japanese encephalitis virus subgenomic replicon to generate single-round infectious particles (SRIPs) that possess designed surface antigens. In the present study, we successfully expanded the capacity of SRIPs to four human-pathogenic mosquito-borne flaviviruses that could potentially be introduced from endemic to non-endemic countries: ZIKV, Sepik virus, Wesselsbron virus, and Usutu virus. Flavivirus-crossreactive monoclonal antibodies dose-dependently neutralized these SRIPs. ZIKV-SRIPs also produced antibody-dose-dependent neutralization curves equivalent to those shown by authentic ZIKV particles using sera from a Zika fever patient. The faithful expression of designed surface antigens on SRIPs will allow their use in neutralization tests to diagnose foreign flaviviral infections. |
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Mahidol University |
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Mahidol University Atsushi Yamanaka Meng Ling Moi Tomohiko Takasaki Ichiro Kurane Mami Matsuda Ryosuke Suzuki Eiji Konishi |
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Article |
author |
Atsushi Yamanaka Meng Ling Moi Tomohiko Takasaki Ichiro Kurane Mami Matsuda Ryosuke Suzuki Eiji Konishi |
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Atsushi Yamanaka |
title |
Utility of Japanese encephalitis virus subgenomic replicon-based single-round infectious particles as antigens in neutralization tests for Zika virus and three other flaviviruses |
title_short |
Utility of Japanese encephalitis virus subgenomic replicon-based single-round infectious particles as antigens in neutralization tests for Zika virus and three other flaviviruses |
title_full |
Utility of Japanese encephalitis virus subgenomic replicon-based single-round infectious particles as antigens in neutralization tests for Zika virus and three other flaviviruses |
title_fullStr |
Utility of Japanese encephalitis virus subgenomic replicon-based single-round infectious particles as antigens in neutralization tests for Zika virus and three other flaviviruses |
title_full_unstemmed |
Utility of Japanese encephalitis virus subgenomic replicon-based single-round infectious particles as antigens in neutralization tests for Zika virus and three other flaviviruses |
title_sort |
utility of japanese encephalitis virus subgenomic replicon-based single-round infectious particles as antigens in neutralization tests for zika virus and three other flaviviruses |
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2018 |
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https://repository.li.mahidol.ac.th/handle/123456789/42811 |
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1763489220486234112 |