Asymptomatic Plasmodium vivax infections induce robust IgG responses to multiple blood-stage proteins in a low-transmission region of western Thailand
© 2017 The Author(s). Background: Thailand is aiming to eliminate malaria by the year 2024. Plasmodium vivax has now become the dominant species causing malaria within the country, and a high proportion of infections are asymptomatic. A better understanding of antibody dynamics to P. vivax antigens...
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th-mahidol.428272019-03-14T15:03:52Z Asymptomatic Plasmodium vivax infections induce robust IgG responses to multiple blood-stage proteins in a low-transmission region of western Thailand Rhea J. Longley Camila T. França Michael T. White Chalermpon Kumpitak Patiwat Sa-Angchai Jakub Gruszczyk Jessica B. Hostetler Anjali Yadava Christopher L. King Rick M. Fairhurst Julian C. Rayner Wai Hong Tham Wang Nguitragool Jetsumon Sattabongkot Ivo Mueller Walter and Eliza Hall Institute of Medical Research Mahidol University University of Melbourne Imperial College London Wellcome Trust Sanger Institute National Institute of Allergy and Infectious Diseases Walter Reed Army Institute of Research Case Western Reserve University Instituto de Salud Global de Barcelona Institut Pasteur, Paris Immunology and Microbiology © 2017 The Author(s). Background: Thailand is aiming to eliminate malaria by the year 2024. Plasmodium vivax has now become the dominant species causing malaria within the country, and a high proportion of infections are asymptomatic. A better understanding of antibody dynamics to P. vivax antigens in a low-transmission setting, where acquired immune responses are poorly characterized, will be pivotal for developing new strategies for elimination, such as improved surveillance methods and vaccines. The objective of this study was to characterize total IgG antibody levels to 11 key P. vivax proteins in a village of western Thailand. Methods: Plasma samples from 546 volunteers enrolled in a cross-sectional survey conducted in 2012 in Kanchanaburi Province were utilized. Total IgG levels to 11 different proteins known or predicted to be involved in reticulocyte binding or invasion (ARP, GAMA, P41, P12, PVX_081550, and five members of the PvRBP family), as well as the leading pre-erythrocytic vaccine candidate (CSP) were measured using a multiplexed bead-based assay. Associations between IgG levels and infection status, age, and spatial location were explored. Results: Individuals from a low-transmission region of western Thailand reacted to all 11 P. vivax recombinant proteins. Significantly greater IgG levels were observed in the presence of a current P. vivax infection, despite all infected individuals being asymptomatic. IgG levels were also higher in adults (18 years and older) than in children. For most of the proteins, higher IgG levels were observed in individuals living closer to the Myanmar border and further away from local health services. Conclusions: Robust IgG responses were observed to most proteins and IgG levels correlated with surrogates of exposure, suggesting these antigens may serve as potential biomarkers of exposure, immunity, or both. 2018-12-21T07:59:51Z 2019-03-14T08:03:52Z 2018-12-21T07:59:51Z 2019-03-14T08:03:52Z 2017-04-28 Article Malaria Journal. Vol.16, No.1 (2017) 10.1186/s12936-017-1826-8 14752875 2-s2.0-85018162350 https://repository.li.mahidol.ac.th/handle/123456789/42827 Mahidol University SCOPUS https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85018162350&origin=inward |
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Immunology and Microbiology Rhea J. Longley Camila T. França Michael T. White Chalermpon Kumpitak Patiwat Sa-Angchai Jakub Gruszczyk Jessica B. Hostetler Anjali Yadava Christopher L. King Rick M. Fairhurst Julian C. Rayner Wai Hong Tham Wang Nguitragool Jetsumon Sattabongkot Ivo Mueller Asymptomatic Plasmodium vivax infections induce robust IgG responses to multiple blood-stage proteins in a low-transmission region of western Thailand |
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© 2017 The Author(s). Background: Thailand is aiming to eliminate malaria by the year 2024. Plasmodium vivax has now become the dominant species causing malaria within the country, and a high proportion of infections are asymptomatic. A better understanding of antibody dynamics to P. vivax antigens in a low-transmission setting, where acquired immune responses are poorly characterized, will be pivotal for developing new strategies for elimination, such as improved surveillance methods and vaccines. The objective of this study was to characterize total IgG antibody levels to 11 key P. vivax proteins in a village of western Thailand. Methods: Plasma samples from 546 volunteers enrolled in a cross-sectional survey conducted in 2012 in Kanchanaburi Province were utilized. Total IgG levels to 11 different proteins known or predicted to be involved in reticulocyte binding or invasion (ARP, GAMA, P41, P12, PVX_081550, and five members of the PvRBP family), as well as the leading pre-erythrocytic vaccine candidate (CSP) were measured using a multiplexed bead-based assay. Associations between IgG levels and infection status, age, and spatial location were explored. Results: Individuals from a low-transmission region of western Thailand reacted to all 11 P. vivax recombinant proteins. Significantly greater IgG levels were observed in the presence of a current P. vivax infection, despite all infected individuals being asymptomatic. IgG levels were also higher in adults (18 years and older) than in children. For most of the proteins, higher IgG levels were observed in individuals living closer to the Myanmar border and further away from local health services. Conclusions: Robust IgG responses were observed to most proteins and IgG levels correlated with surrogates of exposure, suggesting these antigens may serve as potential biomarkers of exposure, immunity, or both. |
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Walter and Eliza Hall Institute of Medical Research |
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Walter and Eliza Hall Institute of Medical Research Rhea J. Longley Camila T. França Michael T. White Chalermpon Kumpitak Patiwat Sa-Angchai Jakub Gruszczyk Jessica B. Hostetler Anjali Yadava Christopher L. King Rick M. Fairhurst Julian C. Rayner Wai Hong Tham Wang Nguitragool Jetsumon Sattabongkot Ivo Mueller |
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Article |
author |
Rhea J. Longley Camila T. França Michael T. White Chalermpon Kumpitak Patiwat Sa-Angchai Jakub Gruszczyk Jessica B. Hostetler Anjali Yadava Christopher L. King Rick M. Fairhurst Julian C. Rayner Wai Hong Tham Wang Nguitragool Jetsumon Sattabongkot Ivo Mueller |
author_sort |
Rhea J. Longley |
title |
Asymptomatic Plasmodium vivax infections induce robust IgG responses to multiple blood-stage proteins in a low-transmission region of western Thailand |
title_short |
Asymptomatic Plasmodium vivax infections induce robust IgG responses to multiple blood-stage proteins in a low-transmission region of western Thailand |
title_full |
Asymptomatic Plasmodium vivax infections induce robust IgG responses to multiple blood-stage proteins in a low-transmission region of western Thailand |
title_fullStr |
Asymptomatic Plasmodium vivax infections induce robust IgG responses to multiple blood-stage proteins in a low-transmission region of western Thailand |
title_full_unstemmed |
Asymptomatic Plasmodium vivax infections induce robust IgG responses to multiple blood-stage proteins in a low-transmission region of western Thailand |
title_sort |
asymptomatic plasmodium vivax infections induce robust igg responses to multiple blood-stage proteins in a low-transmission region of western thailand |
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2018 |
url |
https://repository.li.mahidol.ac.th/handle/123456789/42827 |
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1763497631097552896 |