A Novel Potential Signal Peptide Sequence and Overexpression of ER-Resident Chaperones Enhance Heterologous Protein Secretion in Thermotolerant Methylotrophic Yeast Ogataea thermomethanolica

• Main Authors: Niran Roongsawang, Aekkachai Puseenam, Supattra Kitikhun, Kittapong Sae-Tang, Piyanun Harnpicharnchai, Takao Ohashi, Kazuhito Fujiyama, Witoon Tirasophon, Sutipa Tanapongpipat
• Other Authors: Thailand National Center for Genetic Engineering and Biotechnology
• Format: Article
• Published: 2018
• Subjects: Biochemistry, Genetics and Molecular Biology; Chemical Engineering; Immunology and Microbiology
• Online Access: https://repository.li.mahidol.ac.th/handle/123456789/43155
• Institution: Mahidol University

© 2015, Springer Science+Business Media New York. The thermotolerant methylotrophic yeast Ogataea thermomethanolica is a host for heterologous protein expression via secretion to the culture medium. Efficient secretion is a major bottleneck for heterologous protein production in this strain. To impr...