Lab-on-a-disc for simultaneous determination of total phenolic content and antioxidant activity of beverage samples

• Main Authors: Apichai Phonchai, Yubin Kim, Rattikan Chantiwas, Yoon Kyoung Cho
• Other Authors: Mahidol University
• Format: Article
• Published: 2018
• Subjects: Biochemistry, Genetics and Molecular Biology; Chemical Engineering; Chemistry; Engineering
• Online Access: https://repository.li.mahidol.ac.th/handle/123456789/43268

© 2016 The Royal Society of Chemistry. In this paper, we present a fully integrated and automated lab-on-a-disc for the rapid determination of the total phenolic content (TPC) and antioxidant activity (AA) of beverage samples. The simultaneous determinations of TPC and AA on a spinning disc were achieved by integrating three independent analytical techniques: the Folin-Ciocalteu method that is used to measure TPC, the 2,2-diphenyl-1-picrylhydrazyl radical (DPPH) method and the ferric reducing antioxidant power method that are used to measure AA. The TPC and AA of 8 different beverage samples, including various fruit juices, tea, wine and beer, were analyzed. Unlike conventional labor-intensive processes for measuring TPC and AA, our fully automated platform offers one-step operation and rapid analysis.