Production of carbazole alkaloids through callus and suspension cultures in Clausena harmandiana

© 2018, © 2018 Informa UK Limited, trading as Taylor & Francis Group. Carbazole alkaloids are major constituents in Clausena spp. and exhibit a wide range of biological activities. The roots of Clausena harmandiana are a rich source of active carbazole alkaloids. However, its roots take severa...

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Main Authors: Tharita Kitisripanya, Manus Laoburee, Luksami Puengsiricharoen, Pukpicha Pratoomtong, Supawadee Daodee, Jinda Wangboonskul, Waraporn Putalun
Other Authors: Khon Kaen University
Published: 2019
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Online Access:https://repository.li.mahidol.ac.th/handle/123456789/44885
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spelling th-mahidol.448852019-08-23T17:52:36Z Production of carbazole alkaloids through callus and suspension cultures in Clausena harmandiana Tharita Kitisripanya Manus Laoburee Luksami Puengsiricharoen Pukpicha Pratoomtong Supawadee Daodee Jinda Wangboonskul Waraporn Putalun Khon Kaen University Mahidol University Thammasat University Agricultural and Biological Sciences Biochemistry, Genetics and Molecular Biology Chemistry © 2018, © 2018 Informa UK Limited, trading as Taylor & Francis Group. Carbazole alkaloids are major constituents in Clausena spp. and exhibit a wide range of biological activities. The roots of Clausena harmandiana are a rich source of active carbazole alkaloids. However, its roots take several years to grow to be able to harvest. To obtain an alternative source of carbazole alkaloids, in vitro callus cultures of C. harmandiana were induced, and the formation of two active carbazole alkaloids was investigated. The effects of precursor, concentrations of sucrose, elicitors and light were studied to improve carbazole alkaloids formation. In this study, light had a strong effect on the formation of both carbazole alkaloids. The highest yields of clausine K and 7-methoxymukonal were 4.74 ± 0.26 and 0.92 ± 0.04 mg/g DW, respectively, which have more than 10-fold found in intact roots. According to the results of this study, C. harmandiana callus cultures can be used as an alternative source of carbazole alkaloids for additional biological studies. 2019-08-23T10:21:46Z 2019-08-23T10:21:46Z 2018-01-01 Natural Product Research. (2018) 10.1080/14786419.2018.1533833 14786427 14786419 2-s2.0-85059052930 https://repository.li.mahidol.ac.th/handle/123456789/44885 Mahidol University SCOPUS https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85059052930&origin=inward
institution Mahidol University
building Mahidol University Library
continent Asia
country Thailand
Thailand
content_provider Mahidol University Library
collection Mahidol University Institutional Repository
topic Agricultural and Biological Sciences
Biochemistry, Genetics and Molecular Biology
Chemistry
spellingShingle Agricultural and Biological Sciences
Biochemistry, Genetics and Molecular Biology
Chemistry
Tharita Kitisripanya
Manus Laoburee
Luksami Puengsiricharoen
Pukpicha Pratoomtong
Supawadee Daodee
Jinda Wangboonskul
Waraporn Putalun
Production of carbazole alkaloids through callus and suspension cultures in Clausena harmandiana
description © 2018, © 2018 Informa UK Limited, trading as Taylor & Francis Group. Carbazole alkaloids are major constituents in Clausena spp. and exhibit a wide range of biological activities. The roots of Clausena harmandiana are a rich source of active carbazole alkaloids. However, its roots take several years to grow to be able to harvest. To obtain an alternative source of carbazole alkaloids, in vitro callus cultures of C. harmandiana were induced, and the formation of two active carbazole alkaloids was investigated. The effects of precursor, concentrations of sucrose, elicitors and light were studied to improve carbazole alkaloids formation. In this study, light had a strong effect on the formation of both carbazole alkaloids. The highest yields of clausine K and 7-methoxymukonal were 4.74 ± 0.26 and 0.92 ± 0.04 mg/g DW, respectively, which have more than 10-fold found in intact roots. According to the results of this study, C. harmandiana callus cultures can be used as an alternative source of carbazole alkaloids for additional biological studies.
author2 Khon Kaen University
author_facet Khon Kaen University
Tharita Kitisripanya
Manus Laoburee
Luksami Puengsiricharoen
Pukpicha Pratoomtong
Supawadee Daodee
Jinda Wangboonskul
Waraporn Putalun
author Tharita Kitisripanya
Manus Laoburee
Luksami Puengsiricharoen
Pukpicha Pratoomtong
Supawadee Daodee
Jinda Wangboonskul
Waraporn Putalun
author_sort Tharita Kitisripanya
title Production of carbazole alkaloids through callus and suspension cultures in Clausena harmandiana
title_short Production of carbazole alkaloids through callus and suspension cultures in Clausena harmandiana
title_full Production of carbazole alkaloids through callus and suspension cultures in Clausena harmandiana
title_fullStr Production of carbazole alkaloids through callus and suspension cultures in Clausena harmandiana
title_full_unstemmed Production of carbazole alkaloids through callus and suspension cultures in Clausena harmandiana
title_sort production of carbazole alkaloids through callus and suspension cultures in clausena harmandiana
publishDate 2019
url https://repository.li.mahidol.ac.th/handle/123456789/44885
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