Mosquito vector-associated microbiota: Metabarcoding bacteria and eukaryotic symbionts across habitat types in Thailand endemic for dengue and other arthropod-borne diseases
© 2017 The Authors. Ecology and Evolution published by John Wiley & Sons Ltd. Vector-borne diseases are a major health burden, yet factors affecting their spread are only partially understood. For example, microbial symbionts can impact mosquito reproduction, survival, and vectorial capacity,...
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th-mahidol.449062019-08-23T18:14:43Z Mosquito vector-associated microbiota: Metabarcoding bacteria and eukaryotic symbionts across habitat types in Thailand endemic for dengue and other arthropod-borne diseases Panpim Thongsripong James Angus Chandler Amy B. Green Pattamaporn Kittayapong Bruce A. Wilcox Durrell D. Kapan Shannon N. Bennett California Academy of Sciences University of California, Berkeley Tulane University University of Hawaii at Manoa Mahidol University Agricultural and Biological Sciences Environmental Science © 2017 The Authors. Ecology and Evolution published by John Wiley & Sons Ltd. Vector-borne diseases are a major health burden, yet factors affecting their spread are only partially understood. For example, microbial symbionts can impact mosquito reproduction, survival, and vectorial capacity, and hence affect disease transmission. Nonetheless, current knowledge of mosquito-associated microbial communities is limited. To characterize the bacterial and eukaryotic microbial communities of multiple vector species collected from different habitat types in disease endemic areas, we employed next-generation 454 pyrosequencing of 16S and 18S rRNA amplicon libraries, also known as metabarcoding. We investigated pooled whole adult mosquitoes of three medically important vectors, Aedes aegypti, Ae. albopictus, and Culex quinquefasciatus, collected from different habitats across central Thailand where we previously characterized mosquito diversity. Our results indicate that diversity within the mosquito microbiota is low, with the majority of microbes assigned to one or a few taxa. Two of the most common eukaryotic and bacterial genera recovered (Ascogregarina and Wolbachia, respectively) are known mosquito endosymbionts with potentially parasitic and long evolutionary relationships with their hosts. Patterns of microbial composition and diversity appeared to differ by both vector species and habitat for a given species, although high variability between samples suggests a strong stochastic element to microbiota assembly. In general, our findings suggest that multiple factors, such as habitat condition and mosquito species identity, may influence overall microbial community composition, and thus provide a basis for further investigations into the interactions between vectors, their microbial communities, and human-impacted landscapes that may ultimately affect vector-borne disease risk. 2019-08-23T10:22:27Z 2019-08-23T10:22:27Z 2018-01-01 Article Ecology and Evolution. Vol.8, No.2 (2018), 1352-1368 10.1002/ece3.3676 20457758 2-s2.0-85039034894 https://repository.li.mahidol.ac.th/handle/123456789/44906 Mahidol University SCOPUS https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85039034894&origin=inward |
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Agricultural and Biological Sciences Environmental Science Panpim Thongsripong James Angus Chandler Amy B. Green Pattamaporn Kittayapong Bruce A. Wilcox Durrell D. Kapan Shannon N. Bennett Mosquito vector-associated microbiota: Metabarcoding bacteria and eukaryotic symbionts across habitat types in Thailand endemic for dengue and other arthropod-borne diseases |
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© 2017 The Authors. Ecology and Evolution published by John Wiley & Sons Ltd. Vector-borne diseases are a major health burden, yet factors affecting their spread are only partially understood. For example, microbial symbionts can impact mosquito reproduction, survival, and vectorial capacity, and hence affect disease transmission. Nonetheless, current knowledge of mosquito-associated microbial communities is limited. To characterize the bacterial and eukaryotic microbial communities of multiple vector species collected from different habitat types in disease endemic areas, we employed next-generation 454 pyrosequencing of 16S and 18S rRNA amplicon libraries, also known as metabarcoding. We investigated pooled whole adult mosquitoes of three medically important vectors, Aedes aegypti, Ae. albopictus, and Culex quinquefasciatus, collected from different habitats across central Thailand where we previously characterized mosquito diversity. Our results indicate that diversity within the mosquito microbiota is low, with the majority of microbes assigned to one or a few taxa. Two of the most common eukaryotic and bacterial genera recovered (Ascogregarina and Wolbachia, respectively) are known mosquito endosymbionts with potentially parasitic and long evolutionary relationships with their hosts. Patterns of microbial composition and diversity appeared to differ by both vector species and habitat for a given species, although high variability between samples suggests a strong stochastic element to microbiota assembly. In general, our findings suggest that multiple factors, such as habitat condition and mosquito species identity, may influence overall microbial community composition, and thus provide a basis for further investigations into the interactions between vectors, their microbial communities, and human-impacted landscapes that may ultimately affect vector-borne disease risk. |
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California Academy of Sciences |
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California Academy of Sciences Panpim Thongsripong James Angus Chandler Amy B. Green Pattamaporn Kittayapong Bruce A. Wilcox Durrell D. Kapan Shannon N. Bennett |
format |
Article |
author |
Panpim Thongsripong James Angus Chandler Amy B. Green Pattamaporn Kittayapong Bruce A. Wilcox Durrell D. Kapan Shannon N. Bennett |
author_sort |
Panpim Thongsripong |
title |
Mosquito vector-associated microbiota: Metabarcoding bacteria and eukaryotic symbionts across habitat types in Thailand endemic for dengue and other arthropod-borne diseases |
title_short |
Mosquito vector-associated microbiota: Metabarcoding bacteria and eukaryotic symbionts across habitat types in Thailand endemic for dengue and other arthropod-borne diseases |
title_full |
Mosquito vector-associated microbiota: Metabarcoding bacteria and eukaryotic symbionts across habitat types in Thailand endemic for dengue and other arthropod-borne diseases |
title_fullStr |
Mosquito vector-associated microbiota: Metabarcoding bacteria and eukaryotic symbionts across habitat types in Thailand endemic for dengue and other arthropod-borne diseases |
title_full_unstemmed |
Mosquito vector-associated microbiota: Metabarcoding bacteria and eukaryotic symbionts across habitat types in Thailand endemic for dengue and other arthropod-borne diseases |
title_sort |
mosquito vector-associated microbiota: metabarcoding bacteria and eukaryotic symbionts across habitat types in thailand endemic for dengue and other arthropod-borne diseases |
publishDate |
2019 |
url |
https://repository.li.mahidol.ac.th/handle/123456789/44906 |
_version_ |
1763493900696158208 |