Stimulation of hydrogen photoproduction in Chlorella Sorokiniana subjected to simultaneous nitrogen limitation and sulfur-and/or phosphorus-deprivation
© 2018 The Author(s). Photosynthetic hydrogen (H 2 ) production by green algae has fascinated biologists and energy experts, due to the potential application of this process for renewable energy. In this study, H 2 photoproduction and PSII photochemical activities were investigated in Chlorella sor...
Saved in:
Main Authors: | , , , , , |
---|---|
Other Authors: | |
Format: | Article |
Published: |
2019
|
Subjects: | |
Online Access: | https://repository.li.mahidol.ac.th/handle/123456789/44977 |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
Institution: | Mahidol University |
id |
th-mahidol.44977 |
---|---|
record_format |
dspace |
spelling |
th-mahidol.449772019-08-23T18:16:02Z Stimulation of hydrogen photoproduction in Chlorella Sorokiniana subjected to simultaneous nitrogen limitation and sulfur-and/or phosphorus-deprivation Piyawat Pongpadung Litao Zhang Ramaraj Sathasivam Kittisak Yokthongwattana Niran Juntawong Jianguo Liu Sangmyung University Naresuan University Kasetsart University Institute of Oceanology Chinese Academy of Sciences Mahidol University Biochemistry, Genetics and Molecular Biology Immunology and Microbiology © 2018 The Author(s). Photosynthetic hydrogen (H 2 ) production by green algae has fascinated biologists and energy experts, due to the potential application of this process for renewable energy. In this study, H 2 photoproduction and PSII photochemical activities were investigated in Chlorella sorokiniana exposed to simultaneous nitrogen limitation and sulfur (S-) and/or phosphorus (P-) deprivation. Under S-deprivation, C. sorokiniana produced about 48.2 mL L -1 of H 2 . Moreover, simultaneous nitrogen limitation (0.7 mM NH 4 Cl) and sulfur-and/or phosphorus-deprivation significantly increased H 2 production of C. sorokiniana over that of S-deprivation alone. Maximum H 2 outputs of 77.3, 98.1 and 125.1 mL L -1 were obtained in the N-limited cultures exposed to P-deprivation (TAP-P), S-deprivation (TAP-S) and simultaneous S-and P-deprivation (TAP-S-P), respectively. The average rate of H 2 production for the N-limited culture exposed to TAP-P, TAP-S and TAP-S-P was 1.07, 1.36 and 1.50 mL L -1 h -1 , respectively. Interestingly, the H 2 inducement time in the culture subjected to simultaneous N-limitation and S-and/or P-deprivation was much shorter than that of traditional S-deprivation. The photosynthetic inhibitors, 3-(3, 4-dichlorophenyl)-1, 1-dimethylurea (DCMU) and 2, 5-dibromo-3-methyl-6-isopropylp-benzoquinone (DBMIB) repressed H 2 production in TAP-S-P (0.7 mM NH 4 Cl) medium by 68.04% and 98.65%, respectively. The conditions of simultaneous N-limitation, S-and P-deprivation provided another efficient method for inducing H 2 production in C. sorokiniana. In addition, we also found that two-thirds of the required electrons were generated from the splitting of H 2 O in PSII and that the remaining one-third possibly came from some other substrate catabolism. 2019-08-23T10:24:54Z 2019-08-23T10:24:54Z 2018-12-01 Article Journal of Pure and Applied Microbiology. Vol.12, No.4 (2018), 1719-1727 10.22207/JPAM.12.4.04 2581690X 09737510 2-s2.0-85059464859 https://repository.li.mahidol.ac.th/handle/123456789/44977 Mahidol University SCOPUS https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85059464859&origin=inward |
institution |
Mahidol University |
building |
Mahidol University Library |
continent |
Asia |
country |
Thailand Thailand |
content_provider |
Mahidol University Library |
collection |
Mahidol University Institutional Repository |
topic |
Biochemistry, Genetics and Molecular Biology Immunology and Microbiology |
spellingShingle |
Biochemistry, Genetics and Molecular Biology Immunology and Microbiology Piyawat Pongpadung Litao Zhang Ramaraj Sathasivam Kittisak Yokthongwattana Niran Juntawong Jianguo Liu Stimulation of hydrogen photoproduction in Chlorella Sorokiniana subjected to simultaneous nitrogen limitation and sulfur-and/or phosphorus-deprivation |
description |
© 2018 The Author(s). Photosynthetic hydrogen (H 2 ) production by green algae has fascinated biologists and energy experts, due to the potential application of this process for renewable energy. In this study, H 2 photoproduction and PSII photochemical activities were investigated in Chlorella sorokiniana exposed to simultaneous nitrogen limitation and sulfur (S-) and/or phosphorus (P-) deprivation. Under S-deprivation, C. sorokiniana produced about 48.2 mL L -1 of H 2 . Moreover, simultaneous nitrogen limitation (0.7 mM NH 4 Cl) and sulfur-and/or phosphorus-deprivation significantly increased H 2 production of C. sorokiniana over that of S-deprivation alone. Maximum H 2 outputs of 77.3, 98.1 and 125.1 mL L -1 were obtained in the N-limited cultures exposed to P-deprivation (TAP-P), S-deprivation (TAP-S) and simultaneous S-and P-deprivation (TAP-S-P), respectively. The average rate of H 2 production for the N-limited culture exposed to TAP-P, TAP-S and TAP-S-P was 1.07, 1.36 and 1.50 mL L -1 h -1 , respectively. Interestingly, the H 2 inducement time in the culture subjected to simultaneous N-limitation and S-and/or P-deprivation was much shorter than that of traditional S-deprivation. The photosynthetic inhibitors, 3-(3, 4-dichlorophenyl)-1, 1-dimethylurea (DCMU) and 2, 5-dibromo-3-methyl-6-isopropylp-benzoquinone (DBMIB) repressed H 2 production in TAP-S-P (0.7 mM NH 4 Cl) medium by 68.04% and 98.65%, respectively. The conditions of simultaneous N-limitation, S-and P-deprivation provided another efficient method for inducing H 2 production in C. sorokiniana. In addition, we also found that two-thirds of the required electrons were generated from the splitting of H 2 O in PSII and that the remaining one-third possibly came from some other substrate catabolism. |
author2 |
Sangmyung University |
author_facet |
Sangmyung University Piyawat Pongpadung Litao Zhang Ramaraj Sathasivam Kittisak Yokthongwattana Niran Juntawong Jianguo Liu |
format |
Article |
author |
Piyawat Pongpadung Litao Zhang Ramaraj Sathasivam Kittisak Yokthongwattana Niran Juntawong Jianguo Liu |
author_sort |
Piyawat Pongpadung |
title |
Stimulation of hydrogen photoproduction in Chlorella Sorokiniana subjected to simultaneous nitrogen limitation and sulfur-and/or phosphorus-deprivation |
title_short |
Stimulation of hydrogen photoproduction in Chlorella Sorokiniana subjected to simultaneous nitrogen limitation and sulfur-and/or phosphorus-deprivation |
title_full |
Stimulation of hydrogen photoproduction in Chlorella Sorokiniana subjected to simultaneous nitrogen limitation and sulfur-and/or phosphorus-deprivation |
title_fullStr |
Stimulation of hydrogen photoproduction in Chlorella Sorokiniana subjected to simultaneous nitrogen limitation and sulfur-and/or phosphorus-deprivation |
title_full_unstemmed |
Stimulation of hydrogen photoproduction in Chlorella Sorokiniana subjected to simultaneous nitrogen limitation and sulfur-and/or phosphorus-deprivation |
title_sort |
stimulation of hydrogen photoproduction in chlorella sorokiniana subjected to simultaneous nitrogen limitation and sulfur-and/or phosphorus-deprivation |
publishDate |
2019 |
url |
https://repository.li.mahidol.ac.th/handle/123456789/44977 |
_version_ |
1763495550340038656 |