Unfolded Protein Response (UPR) induced hybrid promoters for heterologous gene expression in pichia pastoris

© 2018, Chiang Mai University. All rights reserved. Inducible promoters constitute a powerful tool in controlling gene expression of heterologous proteins in Pichia pastoris. To expand a native promoter activity, we created an inducible hybrid promoter consisting of a constitutive transcription elo...

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Main Authors: Niwed Kullawong, Sutipa Tanapongpipat, Lily Eurwilaichitr, Witoon Tirasophon
Other Authors: Mahidol University
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Published: 2019
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Online Access:https://repository.li.mahidol.ac.th/handle/123456789/45013
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spelling th-mahidol.450132019-08-28T13:57:14Z Unfolded Protein Response (UPR) induced hybrid promoters for heterologous gene expression in pichia pastoris Niwed Kullawong Sutipa Tanapongpipat Lily Eurwilaichitr Witoon Tirasophon Mahidol University Thailand National Center for Genetic Engineering and Biotechnology Biochemistry, Genetics and Molecular Biology Chemistry Materials Science Mathematics Physics and Astronomy © 2018, Chiang Mai University. All rights reserved. Inducible promoters constitute a powerful tool in controlling gene expression of heterologous proteins in Pichia pastoris. To expand a native promoter activity, we created an inducible hybrid promoter consisting of a constitutive transcription elongation factor 1 ( TEF1 ) promoter (PTEF1) and a 22-bp multimer of unfolded protein response element (UPRE) from Saccharomyces cerevisiae KAR2 gene. The four copies of the UPRE sequence were located immediately upstream to the P TEF1 . The activity of the hybrid promoter for driving expression of green fluorescent protein (GFP) was three to four times higher than its native counterpart without any chemical supplement. Upon adding dithiothreitol (DTT) supplement, the promoter activity increased an additional three-fold within nine hours. The activity was also effective with recombinant human erythropoietin (EPO) expression. Hence this platform may have applications in metabolic engineering, where it can be used as an alternative system for fine-tuning gene expression of heterologous protein in P. pastoris. 2019-08-23T10:26:33Z 2019-08-23T10:26:33Z 2018-11-01 Article Chiang Mai Journal of Science. Vol.45, No.7 (2018), 2554-2565 01252526 2-s2.0-85065773194 https://repository.li.mahidol.ac.th/handle/123456789/45013 Mahidol University SCOPUS https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85065773194&origin=inward
institution Mahidol University
building Mahidol University Library
continent Asia
country Thailand
Thailand
content_provider Mahidol University Library
collection Mahidol University Institutional Repository
topic Biochemistry, Genetics and Molecular Biology
Chemistry
Materials Science
Mathematics
Physics and Astronomy
spellingShingle Biochemistry, Genetics and Molecular Biology
Chemistry
Materials Science
Mathematics
Physics and Astronomy
Niwed Kullawong
Sutipa Tanapongpipat
Lily Eurwilaichitr
Witoon Tirasophon
Unfolded Protein Response (UPR) induced hybrid promoters for heterologous gene expression in pichia pastoris
description © 2018, Chiang Mai University. All rights reserved. Inducible promoters constitute a powerful tool in controlling gene expression of heterologous proteins in Pichia pastoris. To expand a native promoter activity, we created an inducible hybrid promoter consisting of a constitutive transcription elongation factor 1 ( TEF1 ) promoter (PTEF1) and a 22-bp multimer of unfolded protein response element (UPRE) from Saccharomyces cerevisiae KAR2 gene. The four copies of the UPRE sequence were located immediately upstream to the P TEF1 . The activity of the hybrid promoter for driving expression of green fluorescent protein (GFP) was three to four times higher than its native counterpart without any chemical supplement. Upon adding dithiothreitol (DTT) supplement, the promoter activity increased an additional three-fold within nine hours. The activity was also effective with recombinant human erythropoietin (EPO) expression. Hence this platform may have applications in metabolic engineering, where it can be used as an alternative system for fine-tuning gene expression of heterologous protein in P. pastoris.
author2 Mahidol University
author_facet Mahidol University
Niwed Kullawong
Sutipa Tanapongpipat
Lily Eurwilaichitr
Witoon Tirasophon
format Article
author Niwed Kullawong
Sutipa Tanapongpipat
Lily Eurwilaichitr
Witoon Tirasophon
author_sort Niwed Kullawong
title Unfolded Protein Response (UPR) induced hybrid promoters for heterologous gene expression in pichia pastoris
title_short Unfolded Protein Response (UPR) induced hybrid promoters for heterologous gene expression in pichia pastoris
title_full Unfolded Protein Response (UPR) induced hybrid promoters for heterologous gene expression in pichia pastoris
title_fullStr Unfolded Protein Response (UPR) induced hybrid promoters for heterologous gene expression in pichia pastoris
title_full_unstemmed Unfolded Protein Response (UPR) induced hybrid promoters for heterologous gene expression in pichia pastoris
title_sort unfolded protein response (upr) induced hybrid promoters for heterologous gene expression in pichia pastoris
publishDate 2019
url https://repository.li.mahidol.ac.th/handle/123456789/45013
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