Voluntary exercise opposes insulin resistance of skeletal muscle glucose transport during liquid fructose ingestion in rats
© 2018, University of Navarra. We have recently reported that male rats given liquid fructose ingestion exhibit features of cardiometabolic abnormalities including non-obese insulin resistance with impaired insulin signaling transduction in skeletal muscle (Rattanavichit Y et al. Am J Physiol Regul...
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th-mahidol.450852019-08-23T17:29:35Z Voluntary exercise opposes insulin resistance of skeletal muscle glucose transport during liquid fructose ingestion in rats Yupaporn Rattanavichit Jariya Buniam Juthamard Surapongchai Vitoon Saengsirisuwan Mahidol University Biochemistry, Genetics and Molecular Biology © 2018, University of Navarra. We have recently reported that male rats given liquid fructose ingestion exhibit features of cardiometabolic abnormalities including non-obese insulin resistance with impaired insulin signaling transduction in skeletal muscle (Rattanavichit Y et al. Am J Physiol Regul Integr Comp Physiol 311: R1200-R1212, 2016). While exercise can attenuate obesity-related risks of cardiometabolic syndrome, the effectiveness and potential mechanism by which exercise modulates non-obese insulin resistance have not been fully studied. The present investigation evaluated whether regular exercise by voluntary wheel running (VWR) can reduce cardiometabolic risks induced by fructose ingestion. Moreover, the potential cellular adaptations following VWR on key signaling proteins known to influence insulin-induced glucose transport in skeletal muscle of fructose-ingested rats were investigated. Male Sprague-Dawley rats were given either water or liquid fructose (10% wt/vol) without or with access to running wheel for 6 weeks. We demonstrated that VWR restored insulin-stimulated glucose transport in the soleus muscle by improving the functionality of several signaling proteins, including insulin-stimulated IRβ Tyr 1158 /Tyr 1162 /Tyr 1163 (82%), IRS-1 Tyr 989 (112%), Akt Ser 473 (56%), AS160 Thr 642 (76%), and AS160 Ser 588 (82%). These effects were accompanied by lower insulin-stimulated phosphorylation of IRS-1 Ser 307 (37%) and JNK Thr 183 /Tyr 185 (49%), without significant changes in expression of proteins in the renin-angiotensin system. Intriguingly, multiple cardiometabolic abnormalities were not observed in fructose-ingested rats with access to VWR. Collectively, this study demonstrates that the development of cardiometabolic abnormalities as well as insulin resistance of skeletal muscle and defective signaling molecules in rats induced by fructose ingestion could be opposed by VWR. 2019-08-23T10:29:35Z 2019-08-23T10:29:35Z 2018-08-01 Article Journal of Physiology and Biochemistry. Vol.74, No.3 (2018), 455-466 10.1007/s13105-018-0639-8 18778755 11387548 2-s2.0-85048080400 https://repository.li.mahidol.ac.th/handle/123456789/45085 Mahidol University SCOPUS https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85048080400&origin=inward |
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Biochemistry, Genetics and Molecular Biology Yupaporn Rattanavichit Jariya Buniam Juthamard Surapongchai Vitoon Saengsirisuwan Voluntary exercise opposes insulin resistance of skeletal muscle glucose transport during liquid fructose ingestion in rats |
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© 2018, University of Navarra. We have recently reported that male rats given liquid fructose ingestion exhibit features of cardiometabolic abnormalities including non-obese insulin resistance with impaired insulin signaling transduction in skeletal muscle (Rattanavichit Y et al. Am J Physiol Regul Integr Comp Physiol 311: R1200-R1212, 2016). While exercise can attenuate obesity-related risks of cardiometabolic syndrome, the effectiveness and potential mechanism by which exercise modulates non-obese insulin resistance have not been fully studied. The present investigation evaluated whether regular exercise by voluntary wheel running (VWR) can reduce cardiometabolic risks induced by fructose ingestion. Moreover, the potential cellular adaptations following VWR on key signaling proteins known to influence insulin-induced glucose transport in skeletal muscle of fructose-ingested rats were investigated. Male Sprague-Dawley rats were given either water or liquid fructose (10% wt/vol) without or with access to running wheel for 6 weeks. We demonstrated that VWR restored insulin-stimulated glucose transport in the soleus muscle by improving the functionality of several signaling proteins, including insulin-stimulated IRβ Tyr 1158 /Tyr 1162 /Tyr 1163 (82%), IRS-1 Tyr 989 (112%), Akt Ser 473 (56%), AS160 Thr 642 (76%), and AS160 Ser 588 (82%). These effects were accompanied by lower insulin-stimulated phosphorylation of IRS-1 Ser 307 (37%) and JNK Thr 183 /Tyr 185 (49%), without significant changes in expression of proteins in the renin-angiotensin system. Intriguingly, multiple cardiometabolic abnormalities were not observed in fructose-ingested rats with access to VWR. Collectively, this study demonstrates that the development of cardiometabolic abnormalities as well as insulin resistance of skeletal muscle and defective signaling molecules in rats induced by fructose ingestion could be opposed by VWR. |
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Mahidol University |
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Mahidol University Yupaporn Rattanavichit Jariya Buniam Juthamard Surapongchai Vitoon Saengsirisuwan |
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Article |
author |
Yupaporn Rattanavichit Jariya Buniam Juthamard Surapongchai Vitoon Saengsirisuwan |
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Yupaporn Rattanavichit |
title |
Voluntary exercise opposes insulin resistance of skeletal muscle glucose transport during liquid fructose ingestion in rats |
title_short |
Voluntary exercise opposes insulin resistance of skeletal muscle glucose transport during liquid fructose ingestion in rats |
title_full |
Voluntary exercise opposes insulin resistance of skeletal muscle glucose transport during liquid fructose ingestion in rats |
title_fullStr |
Voluntary exercise opposes insulin resistance of skeletal muscle glucose transport during liquid fructose ingestion in rats |
title_full_unstemmed |
Voluntary exercise opposes insulin resistance of skeletal muscle glucose transport during liquid fructose ingestion in rats |
title_sort |
voluntary exercise opposes insulin resistance of skeletal muscle glucose transport during liquid fructose ingestion in rats |
publishDate |
2019 |
url |
https://repository.li.mahidol.ac.th/handle/123456789/45085 |
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1763496774456049664 |