Rh blood phenotyping (D, E, e, C, c) microarrays using multichannel surface plasmon resonance imaging

© 2017 Elsevier B.V. The application of Surface Plasmon Resonance Imaging (SPRi) for the detection of transmembrane antigen of the Rhesus (Rh) blood group system is demonstrated. Clinically significant Rh blood group system antigens, including D, C, E, c, and e, can be simultaneously identified via...

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Main Authors: Chinnawut Pipatpanukul, Sasaki Takeya, Akira Baba, Ratthasart Amarit, Armote Somboonkaew, Boonsong Sutapun, Pimpun Kitpoka, Mongkol Kunakorn, Toemsak Srikhirin
Other Authors: Suranaree University of Technology
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Published: 2019
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Online Access:https://repository.li.mahidol.ac.th/handle/123456789/45186
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spelling th-mahidol.451862019-08-23T18:07:33Z Rh blood phenotyping (D, E, e, C, c) microarrays using multichannel surface plasmon resonance imaging Chinnawut Pipatpanukul Sasaki Takeya Akira Baba Ratthasart Amarit Armote Somboonkaew Boonsong Sutapun Pimpun Kitpoka Mongkol Kunakorn Toemsak Srikhirin Suranaree University of Technology Niigata University Faculty of Medicine, Ramathibodi Hospital, Mahidol University Mahidol University Thailand National Electronics and Computer Technology Center Burapha University Biochemistry, Genetics and Molecular Biology Chemistry Engineering © 2017 Elsevier B.V. The application of Surface Plasmon Resonance Imaging (SPRi) for the detection of transmembrane antigen of the Rhesus (Rh) blood group system is demonstrated. Clinically significant Rh blood group system antigens, including D, C, E, c, and e, can be simultaneously identified via solid phase immobilization assay, which offers significant time savings and assay simplification. Red blood cells (RBCs) flowed through the micro-channel, where a suitable condition for Rh blood group detection was an RBC dilution of 1:10 with a stop-flow condition. Stop flow showed an improvement in specific binding compared to continuous flow. Rh antigens required a longer incubation time to react with the immobilized antibody than A and B antigens due to the difference in antigen type and their location on the RBC. The interaction between the immobilized antibodies and their specific antigenic counterpart on the RBC showed a significant difference in RBC removal behavior using shear flow, measured from the decay of the SPR signal. The strength of the interaction between the immobilized antibody and RBC antigen was determined from the minimum wall shear stress required to start the decay process in the SPR signal. For a given range of immobilized antibody surface densities, the Rh antigen possesses a stronger interaction than A, B, and AB antigens. Identification of 82 samples of ABO and Rh blood groups using SPRi showed good agreement with the standard micro-column agglutination technique. A wider coverage of antigenic recognition for RBC when using the solid phase immobilization assay was demonstrated for the RBC with the antigenic site located on the transmembrane protein of the clinically significant Rh antigen. Given the level of accuracy and precision, the technique showed potential for the detection of the Rh minor blood group system. 2019-08-23T10:34:28Z 2019-08-23T10:34:28Z 2018-04-15 Article Biosensors and Bioelectronics. Vol.102, (2018), 267-275 10.1016/j.bios.2017.10.049 18734235 09565663 2-s2.0-85034616504 https://repository.li.mahidol.ac.th/handle/123456789/45186 Mahidol University SCOPUS https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85034616504&origin=inward
institution Mahidol University
building Mahidol University Library
continent Asia
country Thailand
Thailand
content_provider Mahidol University Library
collection Mahidol University Institutional Repository
topic Biochemistry, Genetics and Molecular Biology
Chemistry
Engineering
spellingShingle Biochemistry, Genetics and Molecular Biology
Chemistry
Engineering
Chinnawut Pipatpanukul
Sasaki Takeya
Akira Baba
Ratthasart Amarit
Armote Somboonkaew
Boonsong Sutapun
Pimpun Kitpoka
Mongkol Kunakorn
Toemsak Srikhirin
Rh blood phenotyping (D, E, e, C, c) microarrays using multichannel surface plasmon resonance imaging
description © 2017 Elsevier B.V. The application of Surface Plasmon Resonance Imaging (SPRi) for the detection of transmembrane antigen of the Rhesus (Rh) blood group system is demonstrated. Clinically significant Rh blood group system antigens, including D, C, E, c, and e, can be simultaneously identified via solid phase immobilization assay, which offers significant time savings and assay simplification. Red blood cells (RBCs) flowed through the micro-channel, where a suitable condition for Rh blood group detection was an RBC dilution of 1:10 with a stop-flow condition. Stop flow showed an improvement in specific binding compared to continuous flow. Rh antigens required a longer incubation time to react with the immobilized antibody than A and B antigens due to the difference in antigen type and their location on the RBC. The interaction between the immobilized antibodies and their specific antigenic counterpart on the RBC showed a significant difference in RBC removal behavior using shear flow, measured from the decay of the SPR signal. The strength of the interaction between the immobilized antibody and RBC antigen was determined from the minimum wall shear stress required to start the decay process in the SPR signal. For a given range of immobilized antibody surface densities, the Rh antigen possesses a stronger interaction than A, B, and AB antigens. Identification of 82 samples of ABO and Rh blood groups using SPRi showed good agreement with the standard micro-column agglutination technique. A wider coverage of antigenic recognition for RBC when using the solid phase immobilization assay was demonstrated for the RBC with the antigenic site located on the transmembrane protein of the clinically significant Rh antigen. Given the level of accuracy and precision, the technique showed potential for the detection of the Rh minor blood group system.
author2 Suranaree University of Technology
author_facet Suranaree University of Technology
Chinnawut Pipatpanukul
Sasaki Takeya
Akira Baba
Ratthasart Amarit
Armote Somboonkaew
Boonsong Sutapun
Pimpun Kitpoka
Mongkol Kunakorn
Toemsak Srikhirin
format Article
author Chinnawut Pipatpanukul
Sasaki Takeya
Akira Baba
Ratthasart Amarit
Armote Somboonkaew
Boonsong Sutapun
Pimpun Kitpoka
Mongkol Kunakorn
Toemsak Srikhirin
author_sort Chinnawut Pipatpanukul
title Rh blood phenotyping (D, E, e, C, c) microarrays using multichannel surface plasmon resonance imaging
title_short Rh blood phenotyping (D, E, e, C, c) microarrays using multichannel surface plasmon resonance imaging
title_full Rh blood phenotyping (D, E, e, C, c) microarrays using multichannel surface plasmon resonance imaging
title_fullStr Rh blood phenotyping (D, E, e, C, c) microarrays using multichannel surface plasmon resonance imaging
title_full_unstemmed Rh blood phenotyping (D, E, e, C, c) microarrays using multichannel surface plasmon resonance imaging
title_sort rh blood phenotyping (d, e, e, c, c) microarrays using multichannel surface plasmon resonance imaging
publishDate 2019
url https://repository.li.mahidol.ac.th/handle/123456789/45186
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