A rhodamine-triazole fluorescent chemodosimeter for Cu²⁺ detection and its application in bioimaging

• Main Authors: Kanokorn Wechakorn, Samran Prabpai, Kanoknetr Suksen, Phongthon Kanjanasirirat, Yongyut Pewkliang, Suparerk Borwornpinyo, Palangpon Kongsaeree
• Other Authors: Mahidol University
• Format: Article
• Published: 2019
• Subjects: Biochemistry, Genetics and Molecular Biology; Chemistry
• Online Access: https://repository.li.mahidol.ac.th/handle/123456789/45250

Copyright © 2017 John Wiley & Sons, Ltd. A rhodamine-based fluorescent chemodosimeter rhodamine hydrazide-triazole (RHT) tethered with a triazole moiety was developed for Cu2+ detection. In aqueous medium, the RHT probe exhibited high selectivity and sensitivity toward Cu2+ among other metal ions. The addition of Cu2+ triggered a fluorescence emission of RHT by 384-fold (Φ = 0.33) based on a ring-opening process and a subsequent hydrolysis reaction. Moreover, RHT also showed a selective colorimetric response toward Cu2+ from colorless solution to pink, readily observed with the naked eye. The limit of detection of RHT for Cu2+ was calculated to be 1 nM (0.06 ppb). RHT was successfully demonstrated to detect Cu2+ in Chang liver cells by confocal fluorescence microscopy.