Protein adsorption and cell behaviors on polycaprolactone film: The effect of surface topography
© 2017 Wiley Periodicals, Inc. Protein adsorption is the first phenomenon that occurs when foreign materials are inserted into the body. Materials used in biomedical applications can have different surface topologies. Knowledge of the effect of the surface on protein adsorption is important due to i...
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th-mahidol.454142019-08-23T18:25:56Z Protein adsorption and cell behaviors on polycaprolactone film: The effect of surface topography Thitikan Khampieng Vipawee Yamassatien Pongpol Ekabutr Prasit Pavasant Pitt Supaphol Chulalongkorn University Mahidol University Chemical Engineering Chemistry Materials Science © 2017 Wiley Periodicals, Inc. Protein adsorption is the first phenomenon that occurs when foreign materials are inserted into the body. Materials used in biomedical applications can have different surface topologies. Knowledge of the effect of the surface on protein adsorption is important due to its influence on cell behavior. The main objective of this study was to analyze polycaprolactone (PCL) films with different surface topologies. Protein adsorption was studied using bovine serum albumin (BSA) as the biomolecule. Different surface topologies of PCL were induced by phase separation using solvents with various solubility parameters. The investigated solvents were chloroform, acetone, tetrahydrofuran (THF), and ethanol (EtOH). The PCL films with different surface topologies and protein-adsorbed PCL films were studied with respect to their hydrophobicity, the concentration and nature of functional groups on their surface, their surface roughness, and their cytotoxicity. Atomic force microscopy revealed that the films with the roughest surface were cast from 40:60 EtOH:THF and contained significantly larger amounts of adsorbed protein. Proteins preferentially adsorbed onto rough surfaces. The cell culture also indicated that mouse-calvaria-derived pre-osteoblastic cells proliferated best and exhibited the greatest amount of calcium deposition on the surface with the largest amount of adsorbed protein. 2019-08-23T10:44:43Z 2019-08-23T10:44:43Z 2018-10-01 Article Advances in Polymer Technology. Vol.37, No.6 (2018), 2030-2042 10.1002/adv.21861 10982329 07306679 2-s2.0-85021350022 https://repository.li.mahidol.ac.th/handle/123456789/45414 Mahidol University SCOPUS https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85021350022&origin=inward |
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Chemical Engineering Chemistry Materials Science Thitikan Khampieng Vipawee Yamassatien Pongpol Ekabutr Prasit Pavasant Pitt Supaphol Protein adsorption and cell behaviors on polycaprolactone film: The effect of surface topography |
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© 2017 Wiley Periodicals, Inc. Protein adsorption is the first phenomenon that occurs when foreign materials are inserted into the body. Materials used in biomedical applications can have different surface topologies. Knowledge of the effect of the surface on protein adsorption is important due to its influence on cell behavior. The main objective of this study was to analyze polycaprolactone (PCL) films with different surface topologies. Protein adsorption was studied using bovine serum albumin (BSA) as the biomolecule. Different surface topologies of PCL were induced by phase separation using solvents with various solubility parameters. The investigated solvents were chloroform, acetone, tetrahydrofuran (THF), and ethanol (EtOH). The PCL films with different surface topologies and protein-adsorbed PCL films were studied with respect to their hydrophobicity, the concentration and nature of functional groups on their surface, their surface roughness, and their cytotoxicity. Atomic force microscopy revealed that the films with the roughest surface were cast from 40:60 EtOH:THF and contained significantly larger amounts of adsorbed protein. Proteins preferentially adsorbed onto rough surfaces. The cell culture also indicated that mouse-calvaria-derived pre-osteoblastic cells proliferated best and exhibited the greatest amount of calcium deposition on the surface with the largest amount of adsorbed protein. |
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Chulalongkorn University |
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Chulalongkorn University Thitikan Khampieng Vipawee Yamassatien Pongpol Ekabutr Prasit Pavasant Pitt Supaphol |
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Thitikan Khampieng Vipawee Yamassatien Pongpol Ekabutr Prasit Pavasant Pitt Supaphol |
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Thitikan Khampieng |
title |
Protein adsorption and cell behaviors on polycaprolactone film: The effect of surface topography |
title_short |
Protein adsorption and cell behaviors on polycaprolactone film: The effect of surface topography |
title_full |
Protein adsorption and cell behaviors on polycaprolactone film: The effect of surface topography |
title_fullStr |
Protein adsorption and cell behaviors on polycaprolactone film: The effect of surface topography |
title_full_unstemmed |
Protein adsorption and cell behaviors on polycaprolactone film: The effect of surface topography |
title_sort |
protein adsorption and cell behaviors on polycaprolactone film: the effect of surface topography |
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2019 |
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https://repository.li.mahidol.ac.th/handle/123456789/45414 |
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1763489269739945984 |