Paper-based amperometric sensor for determination of acetylcholinesterase using screen-printed graphene electrode
© 2017 Elsevier B.V. A simple paper-based sensor with electrochemical detection was first developed for rapid, selective, and sensitive determination of acetylcholinesterase (AChE). The screen-printed graphene electrode was used as working electrode providing sensitivity for the sensor. The amperome...
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th-mahidol.455082019-08-23T17:51:25Z Paper-based amperometric sensor for determination of acetylcholinesterase using screen-printed graphene electrode Yosita Panraksa Weena Siangproh Thitikan Khampieng Orawon Chailapakul Amara Apilux Chulalongkorn University Mahidol University Srinakharinwirot University Chemistry © 2017 Elsevier B.V. A simple paper-based sensor with electrochemical detection was first developed for rapid, selective, and sensitive determination of acetylcholinesterase (AChE). The screen-printed graphene electrode was used as working electrode providing sensitivity for the sensor. The amperometric detection of AChE is based on the determination of thiocholine (TCh) produced from hydrolysis of acetylthiocholine chloride (ATCh) by AChE. To detect TCh, the ATCh immobilized sheet was stacked onto the detection sheet using double adhesive tape, then samples of AChE were dropped on the back side of an ATCh immobilized sheet with only 1 min of incubation time. To avoid interference, glutathione (GSH), the potential of 0.5 V vs. Ag/AgCl was applied onto a graphene electrode and the current, which depends on AChE concentration, was measured. Under optimized conditions, the limit of detection (LOD) from the experiment of AChE determination was 0.1 U/mL with AChE concentration in range of 0.1–15 U/mL. The data correlated well with the data obtained using spectrophotometric method. The developed sensor had been successfully applied to detect AChE in blood samples. 2019-08-23T10:51:25Z 2019-08-23T10:51:25Z 2018-02-01 Article Talanta. Vol.178, (2018), 1017-1023 10.1016/j.talanta.2017.08.096 00399140 2-s2.0-85033345539 https://repository.li.mahidol.ac.th/handle/123456789/45508 Mahidol University SCOPUS https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85033345539&origin=inward |
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Chemistry Yosita Panraksa Weena Siangproh Thitikan Khampieng Orawon Chailapakul Amara Apilux Paper-based amperometric sensor for determination of acetylcholinesterase using screen-printed graphene electrode |
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© 2017 Elsevier B.V. A simple paper-based sensor with electrochemical detection was first developed for rapid, selective, and sensitive determination of acetylcholinesterase (AChE). The screen-printed graphene electrode was used as working electrode providing sensitivity for the sensor. The amperometric detection of AChE is based on the determination of thiocholine (TCh) produced from hydrolysis of acetylthiocholine chloride (ATCh) by AChE. To detect TCh, the ATCh immobilized sheet was stacked onto the detection sheet using double adhesive tape, then samples of AChE were dropped on the back side of an ATCh immobilized sheet with only 1 min of incubation time. To avoid interference, glutathione (GSH), the potential of 0.5 V vs. Ag/AgCl was applied onto a graphene electrode and the current, which depends on AChE concentration, was measured. Under optimized conditions, the limit of detection (LOD) from the experiment of AChE determination was 0.1 U/mL with AChE concentration in range of 0.1–15 U/mL. The data correlated well with the data obtained using spectrophotometric method. The developed sensor had been successfully applied to detect AChE in blood samples. |
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Chulalongkorn University |
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Chulalongkorn University Yosita Panraksa Weena Siangproh Thitikan Khampieng Orawon Chailapakul Amara Apilux |
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Yosita Panraksa Weena Siangproh Thitikan Khampieng Orawon Chailapakul Amara Apilux |
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Yosita Panraksa |
title |
Paper-based amperometric sensor for determination of acetylcholinesterase using screen-printed graphene electrode |
title_short |
Paper-based amperometric sensor for determination of acetylcholinesterase using screen-printed graphene electrode |
title_full |
Paper-based amperometric sensor for determination of acetylcholinesterase using screen-printed graphene electrode |
title_fullStr |
Paper-based amperometric sensor for determination of acetylcholinesterase using screen-printed graphene electrode |
title_full_unstemmed |
Paper-based amperometric sensor for determination of acetylcholinesterase using screen-printed graphene electrode |
title_sort |
paper-based amperometric sensor for determination of acetylcholinesterase using screen-printed graphene electrode |
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2019 |
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https://repository.li.mahidol.ac.th/handle/123456789/45508 |
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1763494002237112320 |