Bacterial Diversity and Phylogenetic Analysis of Type II Polyketide Synthase Gene from Manao-Pee Cave, Thailand
© 2018 Informa UK Limited, trading as Taylor & Francis Group. A subterranean limestone cave, Manao-Pee, was investigated for bacterial diversity and potential secondary metabolites production. Comparative 16 S rRNA analysis revealed that cave soil was highly dominated by Actinobacteria; wherea...
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| Main Authors: | , , , , , |
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| Other Authors: | |
| Format: | Article |
| Published: |
2019
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| Subjects: | |
| Online Access: | https://repository.li.mahidol.ac.th/handle/123456789/45737 |
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| Institution: | Mahidol University |
| Summary: | © 2018 Informa UK Limited, trading as Taylor & Francis Group. A subterranean limestone cave, Manao-Pee, was investigated for bacterial diversity and potential secondary metabolites production. Comparative 16 S rRNA analysis revealed that cave soil was highly dominated by Actinobacteria; whereas, Proteobacteria was highly abundant outside the cave. As Actinobacteria are biotechnologically valuable for their secondary metabolites, the diversity of the β-ketoacyl synthase (KSβ) was investigated. The results showed that the identified KSβhas 61–80% amino acid sequence identity to known sequences. Phylogenetic analysis placed some of the sequences in novel clades, suggesting the presence of novel KSβdomains. Thus, Manao-Pee cave is a promising habitat to discover potential novel bioactive compounds. |
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