Aptamer-functionalized DNA nanosphere as a stimuli-responsive nanocarrier
© 2017 Elsevier B.V. Smart nanocarriers that can respond to specific signals have recently gained attention in clinical research. Through the scaffolded DNA origami technique, we developed an aptamer-functionalized DNA nanosphere that could change its conformation upon binding to cancer-specific Muc...
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th-mahidol.458172019-08-28T13:59:39Z Aptamer-functionalized DNA nanosphere as a stimuli-responsive nanocarrier Supattra Chaithongyot Nusara Chomanee Komgrid Charngkaew Anuttara Udomprasert Thaned Kangsamaksin Mahidol University Faculty of Medicine, Siriraj Hospital, Mahidol University Burapha University Engineering Materials Science Physics and Astronomy © 2017 Elsevier B.V. Smart nanocarriers that can respond to specific signals have recently gained attention in clinical research. Through the scaffolded DNA origami technique, we developed an aptamer-functionalized DNA nanosphere that could change its conformation upon binding to cancer-specific Mucin 1 protein (MUC1). A MUC1 aptamer was added into the lock-and-key system of the DNA nanosphere, and specific binding of the aptamer and MUC1 triggered the opening of the structure. A decrease in FRET signals demonstrated that the DNA nanosphere underwent structural change to the open conformation in response to the MUC1-containing cell lysate. Gold nanoparticles were also successfully encapsulated inside the cavity, implicating its loading ability. Our data confirmed that the aptamer-modified DNA nanosphere has proved to be a responsive nanocarrier and may serve as a promising candidate for cancer theranostic purposes. 2019-08-23T11:08:07Z 2019-08-23T11:08:07Z 2018-03-01 Article Materials Letters. Vol.214, (2018), 72-75 10.1016/j.matlet.2017.11.118 18734979 0167577X 2-s2.0-85037117866 https://repository.li.mahidol.ac.th/handle/123456789/45817 Mahidol University SCOPUS https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85037117866&origin=inward |
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Engineering Materials Science Physics and Astronomy Supattra Chaithongyot Nusara Chomanee Komgrid Charngkaew Anuttara Udomprasert Thaned Kangsamaksin Aptamer-functionalized DNA nanosphere as a stimuli-responsive nanocarrier |
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© 2017 Elsevier B.V. Smart nanocarriers that can respond to specific signals have recently gained attention in clinical research. Through the scaffolded DNA origami technique, we developed an aptamer-functionalized DNA nanosphere that could change its conformation upon binding to cancer-specific Mucin 1 protein (MUC1). A MUC1 aptamer was added into the lock-and-key system of the DNA nanosphere, and specific binding of the aptamer and MUC1 triggered the opening of the structure. A decrease in FRET signals demonstrated that the DNA nanosphere underwent structural change to the open conformation in response to the MUC1-containing cell lysate. Gold nanoparticles were also successfully encapsulated inside the cavity, implicating its loading ability. Our data confirmed that the aptamer-modified DNA nanosphere has proved to be a responsive nanocarrier and may serve as a promising candidate for cancer theranostic purposes. |
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Mahidol University |
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Mahidol University Supattra Chaithongyot Nusara Chomanee Komgrid Charngkaew Anuttara Udomprasert Thaned Kangsamaksin |
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Article |
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Supattra Chaithongyot Nusara Chomanee Komgrid Charngkaew Anuttara Udomprasert Thaned Kangsamaksin |
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Supattra Chaithongyot |
title |
Aptamer-functionalized DNA nanosphere as a stimuli-responsive nanocarrier |
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Aptamer-functionalized DNA nanosphere as a stimuli-responsive nanocarrier |
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Aptamer-functionalized DNA nanosphere as a stimuli-responsive nanocarrier |
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Aptamer-functionalized DNA nanosphere as a stimuli-responsive nanocarrier |
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Aptamer-functionalized DNA nanosphere as a stimuli-responsive nanocarrier |
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aptamer-functionalized dna nanosphere as a stimuli-responsive nanocarrier |
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2019 |
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https://repository.li.mahidol.ac.th/handle/123456789/45817 |
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