Hamster IFN-γ+CD4+ and IL-4+CD4+ T cell responses against leptospires are significantly higher than those of mice

BACKGROUND: Leptospirosis is a bacterial disease caused by the Leptospira interrogans. The hamster is considered a susceptible host while the mouse is resistant. The knowledge of hamster T cell immunity is limited compared to the mouse. The reason why the hamster and the mouse give different respons...

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Main Authors: Yaowarin Nakornpakdee, Rasana W. Sermswan, Santi Maneewatchararangsri, Surasakdi Wongratanacheewin
Other Authors: Khon Kaen University
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Published: 2019
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Online Access:https://repository.li.mahidol.ac.th/handle/123456789/45925
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spelling th-mahidol.459252019-08-23T18:33:06Z Hamster IFN-γ+CD4+ and IL-4+CD4+ T cell responses against leptospires are significantly higher than those of mice Yaowarin Nakornpakdee Rasana W. Sermswan Santi Maneewatchararangsri Surasakdi Wongratanacheewin Khon Kaen University Mahidol University Immunology and Microbiology Medicine BACKGROUND: Leptospirosis is a bacterial disease caused by the Leptospira interrogans. The hamster is considered a susceptible host while the mouse is resistant. The knowledge of hamster T cell immunity is limited compared to the mouse. The reason why the hamster and the mouse give different responses to leptospires remains unclear. OBJECTIVE: To determine the differential responses of CD4+ T cells between hamsters and mice using Leptospira interrogans as an infectious model. METHODS: The CD4+ T-cell reactivity and their intracellular cytokine responses after infection with live L.interrogans serovar Autumnalis or leptospiral antigens, or injection with recombinant LipL32 protein (rLipL32) were elucidated. For secondary immune responses, mononuclear cells were re-stimulated with leptospiral crude antigens (LAg) or rLipL32. Intracellular cytokines and CD4+ T cells were determined using flow cytometry. RESULTS: There were no significant differences between the percentages of hamster and mouse CD4+ and CD25+CD4+ T cell responses to live bacteria. Mouse CD4+ (24.50±1.98%) and CD25+CD4+ T cells (3.83±0.88) responded significantly higher than those of hamster (15.07±2.82% and 2.00±0.37%) when infected and re-stimulated with LAg. The numbers of IFN-γ and IL-4 producing cells in hamsters at 1.76±0.10% and 0.82±0.25% for IFN-γ+CD4+ and IL-4+CD4+ T cells were significantly higher than those in resistant mice at 0.10±0.02% and 0.23±0.03% for IFN-γ+CD4+ and IL-4+CD4+ T cells. CONCLUSION: Hamsters responded significantly higher in secondary stimulation especially in the levels of the IFN-γ+ and IL-4+CD4+ T cells. The mechanisms of this dissimilarity remain to be elucidated. 2019-08-23T11:15:47Z 2019-08-23T11:15:47Z 2018-12-01 Article Asian Pacific journal of allergy and immunology. Vol.36, No.4 (2018), 265-272 10.12932/AP-130917-0158 0125877X 2-s2.0-85059287034 https://repository.li.mahidol.ac.th/handle/123456789/45925 Mahidol University SCOPUS https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85059287034&origin=inward
institution Mahidol University
building Mahidol University Library
continent Asia
country Thailand
Thailand
content_provider Mahidol University Library
collection Mahidol University Institutional Repository
topic Immunology and Microbiology
Medicine
spellingShingle Immunology and Microbiology
Medicine
Yaowarin Nakornpakdee
Rasana W. Sermswan
Santi Maneewatchararangsri
Surasakdi Wongratanacheewin
Hamster IFN-γ+CD4+ and IL-4+CD4+ T cell responses against leptospires are significantly higher than those of mice
description BACKGROUND: Leptospirosis is a bacterial disease caused by the Leptospira interrogans. The hamster is considered a susceptible host while the mouse is resistant. The knowledge of hamster T cell immunity is limited compared to the mouse. The reason why the hamster and the mouse give different responses to leptospires remains unclear. OBJECTIVE: To determine the differential responses of CD4+ T cells between hamsters and mice using Leptospira interrogans as an infectious model. METHODS: The CD4+ T-cell reactivity and their intracellular cytokine responses after infection with live L.interrogans serovar Autumnalis or leptospiral antigens, or injection with recombinant LipL32 protein (rLipL32) were elucidated. For secondary immune responses, mononuclear cells were re-stimulated with leptospiral crude antigens (LAg) or rLipL32. Intracellular cytokines and CD4+ T cells were determined using flow cytometry. RESULTS: There were no significant differences between the percentages of hamster and mouse CD4+ and CD25+CD4+ T cell responses to live bacteria. Mouse CD4+ (24.50±1.98%) and CD25+CD4+ T cells (3.83±0.88) responded significantly higher than those of hamster (15.07±2.82% and 2.00±0.37%) when infected and re-stimulated with LAg. The numbers of IFN-γ and IL-4 producing cells in hamsters at 1.76±0.10% and 0.82±0.25% for IFN-γ+CD4+ and IL-4+CD4+ T cells were significantly higher than those in resistant mice at 0.10±0.02% and 0.23±0.03% for IFN-γ+CD4+ and IL-4+CD4+ T cells. CONCLUSION: Hamsters responded significantly higher in secondary stimulation especially in the levels of the IFN-γ+ and IL-4+CD4+ T cells. The mechanisms of this dissimilarity remain to be elucidated.
author2 Khon Kaen University
author_facet Khon Kaen University
Yaowarin Nakornpakdee
Rasana W. Sermswan
Santi Maneewatchararangsri
Surasakdi Wongratanacheewin
format Article
author Yaowarin Nakornpakdee
Rasana W. Sermswan
Santi Maneewatchararangsri
Surasakdi Wongratanacheewin
author_sort Yaowarin Nakornpakdee
title Hamster IFN-γ+CD4+ and IL-4+CD4+ T cell responses against leptospires are significantly higher than those of mice
title_short Hamster IFN-γ+CD4+ and IL-4+CD4+ T cell responses against leptospires are significantly higher than those of mice
title_full Hamster IFN-γ+CD4+ and IL-4+CD4+ T cell responses against leptospires are significantly higher than those of mice
title_fullStr Hamster IFN-γ+CD4+ and IL-4+CD4+ T cell responses against leptospires are significantly higher than those of mice
title_full_unstemmed Hamster IFN-γ+CD4+ and IL-4+CD4+ T cell responses against leptospires are significantly higher than those of mice
title_sort hamster ifn-γ+cd4+ and il-4+cd4+ t cell responses against leptospires are significantly higher than those of mice
publishDate 2019
url https://repository.li.mahidol.ac.th/handle/123456789/45925
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