Presence of B. thailandensis and B. thailandensis expressing B. pseudomallei-like capsular polysaccharide in Thailand, and their associations with serological response to B. pseudomallei

© 2018 Hantrakun et al. Background: Burkholderia pseudomallei is an environmental Gram-negative bacillus and the cause of melioidosis. B. thailandensis, some strains of which express a B. pseudomallei-like capsular polysaccharide (BTCV), is also commonly found in the environment in Southeast Asia bu...

Full description

Saved in:
Bibliographic Details
Main Authors: Viriya Hantrakun, Janjira Thaipadungpanit, Patpong Rongkard, Prapaporn Srilohasin, Premjit Amornchai, Sayan Langla, Mavuto Mukaka, Narisara Chantratita, Vanaporn Wuthiekanun, David A.B. Dance, Nicholas P.J. Day, Sharon J. Peacock, Direk Limmathurotsakul
Other Authors: London School of Hygiene & Tropical Medicine
Format: Article
Published: 2019
Subjects:
Online Access:https://repository.li.mahidol.ac.th/handle/123456789/47220
Tags: Add Tag
No Tags, Be the first to tag this record!
Institution: Mahidol University
id th-mahidol.47220
record_format dspace
spelling th-mahidol.472202019-08-28T13:38:29Z Presence of B. thailandensis and B. thailandensis expressing B. pseudomallei-like capsular polysaccharide in Thailand, and their associations with serological response to B. pseudomallei Viriya Hantrakun Janjira Thaipadungpanit Patpong Rongkard Prapaporn Srilohasin Premjit Amornchai Sayan Langla Mavuto Mukaka Narisara Chantratita Vanaporn Wuthiekanun David A.B. Dance Nicholas P.J. Day Sharon J. Peacock Direk Limmathurotsakul London School of Hygiene &amp; Tropical Medicine University of Cambridge Mahidol University Nuffield Department of Clinical Medicine Mahosot Hospital Medicine © 2018 Hantrakun et al. Background: Burkholderia pseudomallei is an environmental Gram-negative bacillus and the cause of melioidosis. B. thailandensis, some strains of which express a B. pseudomallei-like capsular polysaccharide (BTCV), is also commonly found in the environment in Southeast Asia but is considered non-pathogenic. The aim of the study was to determine the distribution of B. thailandensis and its capsular variant in Thailand and investigate whether its presence is associated with a serological response to B. pseudomallei. Methodology/principal findings: We evaluated the presence of B. pseudomallei and B. thailandensis in 61 rice fields in Northeast (n = 21), East (n = 19) and Central (n = 21) Thailand. We found BTCV in rice fields in East and Central but not Northeast Thailand. Fourteen fields were culture positive for B. pseudomallei alone, 8 for B. thailandensis alone, 11 for both B. pseudomallei and B. thailandensis, 6 for both B. thailandensis and BTCV, and 5 for B. pseudomallei, B. thailandensis and BTCV. Serological testing using the indirect hemagglutination assay (IHA) of 96 farmers who worked in the study fields demonstrated that farmers who worked in B. pseudomallei-positive fields had higher IHA titers than those who worked in B. pseudomallei-negative fields (median 1:40 [range: <1:10–1:640] vs. <1:10 [range: <1:10–1:320], p = 0.002). In a multivariable ordered logistic regression model, IHA titers were significantly associated with the presence of B. pseudomallei (aOR = 3.7; 95% CI 1.8–7.8, p = 0.001) but were not associated with presence of B. thailandensis (p = 0.32) or BTCV (p = 0.32). One sequence type (696) was identified for the 27 BTCV isolates tested. Conclusions/significance: This is the first report of BTCV in Thailand. The presence of B. pseudomallei and B. thailandensis in the same field was not uncommon. Our findings suggest that IHA positivity of healthy rice farmers in Thailand is associated with the presence of B. pseudomallei in rice fields rather than B. thailandensis or BTCV. 2019-08-28T06:38:29Z 2019-08-28T06:38:29Z 2018-01-01 Article PLoS Neglected Tropical Diseases. Vol.12, No.1 (2018) 10.1371/journal.pntd.0006193 19352735 19352727 2-s2.0-85041734272 https://repository.li.mahidol.ac.th/handle/123456789/47220 Mahidol University SCOPUS https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85041734272&origin=inward
institution Mahidol University
building Mahidol University Library
continent Asia
country Thailand
Thailand
content_provider Mahidol University Library
collection Mahidol University Institutional Repository
topic Medicine
spellingShingle Medicine
Viriya Hantrakun
Janjira Thaipadungpanit
Patpong Rongkard
Prapaporn Srilohasin
Premjit Amornchai
Sayan Langla
Mavuto Mukaka
Narisara Chantratita
Vanaporn Wuthiekanun
David A.B. Dance
Nicholas P.J. Day
Sharon J. Peacock
Direk Limmathurotsakul
Presence of B. thailandensis and B. thailandensis expressing B. pseudomallei-like capsular polysaccharide in Thailand, and their associations with serological response to B. pseudomallei
description © 2018 Hantrakun et al. Background: Burkholderia pseudomallei is an environmental Gram-negative bacillus and the cause of melioidosis. B. thailandensis, some strains of which express a B. pseudomallei-like capsular polysaccharide (BTCV), is also commonly found in the environment in Southeast Asia but is considered non-pathogenic. The aim of the study was to determine the distribution of B. thailandensis and its capsular variant in Thailand and investigate whether its presence is associated with a serological response to B. pseudomallei. Methodology/principal findings: We evaluated the presence of B. pseudomallei and B. thailandensis in 61 rice fields in Northeast (n = 21), East (n = 19) and Central (n = 21) Thailand. We found BTCV in rice fields in East and Central but not Northeast Thailand. Fourteen fields were culture positive for B. pseudomallei alone, 8 for B. thailandensis alone, 11 for both B. pseudomallei and B. thailandensis, 6 for both B. thailandensis and BTCV, and 5 for B. pseudomallei, B. thailandensis and BTCV. Serological testing using the indirect hemagglutination assay (IHA) of 96 farmers who worked in the study fields demonstrated that farmers who worked in B. pseudomallei-positive fields had higher IHA titers than those who worked in B. pseudomallei-negative fields (median 1:40 [range: <1:10–1:640] vs. <1:10 [range: <1:10–1:320], p = 0.002). In a multivariable ordered logistic regression model, IHA titers were significantly associated with the presence of B. pseudomallei (aOR = 3.7; 95% CI 1.8–7.8, p = 0.001) but were not associated with presence of B. thailandensis (p = 0.32) or BTCV (p = 0.32). One sequence type (696) was identified for the 27 BTCV isolates tested. Conclusions/significance: This is the first report of BTCV in Thailand. The presence of B. pseudomallei and B. thailandensis in the same field was not uncommon. Our findings suggest that IHA positivity of healthy rice farmers in Thailand is associated with the presence of B. pseudomallei in rice fields rather than B. thailandensis or BTCV.
author2 London School of Hygiene &amp; Tropical Medicine
author_facet London School of Hygiene &amp; Tropical Medicine
Viriya Hantrakun
Janjira Thaipadungpanit
Patpong Rongkard
Prapaporn Srilohasin
Premjit Amornchai
Sayan Langla
Mavuto Mukaka
Narisara Chantratita
Vanaporn Wuthiekanun
David A.B. Dance
Nicholas P.J. Day
Sharon J. Peacock
Direk Limmathurotsakul
format Article
author Viriya Hantrakun
Janjira Thaipadungpanit
Patpong Rongkard
Prapaporn Srilohasin
Premjit Amornchai
Sayan Langla
Mavuto Mukaka
Narisara Chantratita
Vanaporn Wuthiekanun
David A.B. Dance
Nicholas P.J. Day
Sharon J. Peacock
Direk Limmathurotsakul
author_sort Viriya Hantrakun
title Presence of B. thailandensis and B. thailandensis expressing B. pseudomallei-like capsular polysaccharide in Thailand, and their associations with serological response to B. pseudomallei
title_short Presence of B. thailandensis and B. thailandensis expressing B. pseudomallei-like capsular polysaccharide in Thailand, and their associations with serological response to B. pseudomallei
title_full Presence of B. thailandensis and B. thailandensis expressing B. pseudomallei-like capsular polysaccharide in Thailand, and their associations with serological response to B. pseudomallei
title_fullStr Presence of B. thailandensis and B. thailandensis expressing B. pseudomallei-like capsular polysaccharide in Thailand, and their associations with serological response to B. pseudomallei
title_full_unstemmed Presence of B. thailandensis and B. thailandensis expressing B. pseudomallei-like capsular polysaccharide in Thailand, and their associations with serological response to B. pseudomallei
title_sort presence of b. thailandensis and b. thailandensis expressing b. pseudomallei-like capsular polysaccharide in thailand, and their associations with serological response to b. pseudomallei
publishDate 2019
url https://repository.li.mahidol.ac.th/handle/123456789/47220
_version_ 1763496663347888128