Time-dependent distribution of SN-38 from injectable polymeric depots in brain tumor model

© 2018 IOP Publishing Ltd. Distribution study of drugs in tissues is essential to provide the information on the accumulation and penetration of drugs especially anticancer drugs from the local drug delivery system. Fluorescence and histophotological images of drug-treated tissues were used to exam...

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Main Authors: Chawan Manaspon, Khuanjit Chaimongkolnukul, Kanchana Kengkoom, Atthaporn Boongird, Suradej Hongeng, Arthit Chairoungdua, Norased Nasongkla
Other Authors: Faculty of Medicine, Ramathibodi Hospital, Mahidol University
Format: Article
Published: 2019
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Online Access:https://repository.li.mahidol.ac.th/handle/123456789/47278
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spelling th-mahidol.472782022-09-02T16:17:12Z Time-dependent distribution of SN-38 from injectable polymeric depots in brain tumor model Chawan Manaspon Khuanjit Chaimongkolnukul Kanchana Kengkoom Atthaporn Boongird Suradej Hongeng Arthit Chairoungdua Norased Nasongkla Faculty of Medicine, Ramathibodi Hospital, Mahidol University Mahidol University Nursing © 2018 IOP Publishing Ltd. Distribution study of drugs in tissues is essential to provide the information on the accumulation and penetration of drugs especially anticancer drugs from the local drug delivery system. Fluorescence and histophotological images of drug-treated tissues were used to examine drug distribution and accumulation in tumors. This work investigated the distribution profiles of SN-38 in U-87MG-bearing mice received SN-38-loaded depots via intratumoral injection. Distribution profile of SN-38 was followed-up by its self-fluorescent property. Depots comprised of three main components including PLEC (poly(D,L-lactide-random-ϵ-caprolactone)-block-poly(ethylene glycol)-block-poly(D,L-lactide-random-ϵ-caprolactone)) copolymer, glycofurol, and SN-38. Depots containing 1.6 ± 0.4 mg of SN-38 were injected into the tumors (200-250 mm 3 ) and monitored up to 18 days post-injection. Tumors at each time point were sectioned and images were acquired with fluorescence microscope. Results showed that SN-38 penetrated into the tumors at the distance of 0.75 mm from the boundary of depots. Distributed SN-38 provided the necrotic area surrounding the depots. Fluorescence images were reconstructed and showed as 3D images. High level of SN-38 accumulation in tumors was found up to day 18 post-injection at 35.4 μg g -1 of tumors. This result can confirm and explain the distribution and penetration of SN-38. The information will be valuable to the design of depots administration in clinical trial. 2019-08-28T06:45:18Z 2019-08-28T06:45:18Z 2018-07-27 Article Biomedical Physics and Engineering Express. Vol.4, No.5 (2018) 10.1088/2057-1976/aad396 20571976 2-s2.0-85053154844 https://repository.li.mahidol.ac.th/handle/123456789/47278 Mahidol University SCOPUS https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85053154844&origin=inward
institution Mahidol University
building Mahidol University Library
continent Asia
country Thailand
Thailand
content_provider Mahidol University Library
collection Mahidol University Institutional Repository
topic Nursing
spellingShingle Nursing
Chawan Manaspon
Khuanjit Chaimongkolnukul
Kanchana Kengkoom
Atthaporn Boongird
Suradej Hongeng
Arthit Chairoungdua
Norased Nasongkla
Time-dependent distribution of SN-38 from injectable polymeric depots in brain tumor model
description © 2018 IOP Publishing Ltd. Distribution study of drugs in tissues is essential to provide the information on the accumulation and penetration of drugs especially anticancer drugs from the local drug delivery system. Fluorescence and histophotological images of drug-treated tissues were used to examine drug distribution and accumulation in tumors. This work investigated the distribution profiles of SN-38 in U-87MG-bearing mice received SN-38-loaded depots via intratumoral injection. Distribution profile of SN-38 was followed-up by its self-fluorescent property. Depots comprised of three main components including PLEC (poly(D,L-lactide-random-ϵ-caprolactone)-block-poly(ethylene glycol)-block-poly(D,L-lactide-random-ϵ-caprolactone)) copolymer, glycofurol, and SN-38. Depots containing 1.6 ± 0.4 mg of SN-38 were injected into the tumors (200-250 mm 3 ) and monitored up to 18 days post-injection. Tumors at each time point were sectioned and images were acquired with fluorescence microscope. Results showed that SN-38 penetrated into the tumors at the distance of 0.75 mm from the boundary of depots. Distributed SN-38 provided the necrotic area surrounding the depots. Fluorescence images were reconstructed and showed as 3D images. High level of SN-38 accumulation in tumors was found up to day 18 post-injection at 35.4 μg g -1 of tumors. This result can confirm and explain the distribution and penetration of SN-38. The information will be valuable to the design of depots administration in clinical trial.
author2 Faculty of Medicine, Ramathibodi Hospital, Mahidol University
author_facet Faculty of Medicine, Ramathibodi Hospital, Mahidol University
Chawan Manaspon
Khuanjit Chaimongkolnukul
Kanchana Kengkoom
Atthaporn Boongird
Suradej Hongeng
Arthit Chairoungdua
Norased Nasongkla
format Article
author Chawan Manaspon
Khuanjit Chaimongkolnukul
Kanchana Kengkoom
Atthaporn Boongird
Suradej Hongeng
Arthit Chairoungdua
Norased Nasongkla
author_sort Chawan Manaspon
title Time-dependent distribution of SN-38 from injectable polymeric depots in brain tumor model
title_short Time-dependent distribution of SN-38 from injectable polymeric depots in brain tumor model
title_full Time-dependent distribution of SN-38 from injectable polymeric depots in brain tumor model
title_fullStr Time-dependent distribution of SN-38 from injectable polymeric depots in brain tumor model
title_full_unstemmed Time-dependent distribution of SN-38 from injectable polymeric depots in brain tumor model
title_sort time-dependent distribution of sn-38 from injectable polymeric depots in brain tumor model
publishDate 2019
url https://repository.li.mahidol.ac.th/handle/123456789/47278
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